{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,2]],"date-time":"2026-07-02T10:27:29Z","timestamp":1782988049096,"version":"3.54.5"},"reference-count":21,"publisher":"Wiley","issue":"4-5","license":[{"start":{"date-parts":[[2022,6,3]],"date-time":"2022-06-03T00:00:00Z","timestamp":1654214400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["aocs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Lipids"],"published-print":{"date-parts":[[2022,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Validated reference values and procedures are needed to ensure optimal diagnosis of dyslipidemia in sub\u2010Saharan Africa. We aimed to validate an analysis method and establish reference intervals of lipid profile parameters in Cameroonians using this method. On a cross\u2010sectional study conducted from November 2019 to August 2020 in Yaound\u00e9, we have analyzed blood samples with Cobas\u00ae 6000. We subscribed to ASQUALAB's External Quality Assessments (EQA) and Outsourced Internal Quality Controls (IQC). Reproducibility, repeatability, correctness accuracy and uncertainty were evaluated using IQC. Consenting adult participants were conveniently sampled, excluding those with any condition that may affect lipid profile. Descriptive statistics were reported accordingly, agreement was assessed with Bland\u2013Altman analysis, and reference intervals were defined according to CLSI and IFCC recommendations. The coefficients of variation for repeatability, reproducibility, and correctness bias ranged between 0.6% and 6%, with all values within the normal range. Expanded uncertainty of total cholesterol, HDL and triglycerides measurements were, respectively, 0.45, 0.24 and 0.18. We included 422 participants with a mean age of 30.2 (10.9) years and 248 (58.8%) females. Reference intervals for total cholesterol, HDL, triglycerides and LDL were, respectively, 2.94\u20136.02\u2009mmol\/L, 0.90\u20132.06\u2009mmol\/L, 0.35\u20131.36\u2009mmol\/L, 1.37\u20134.13\u2009mmol\/L. These intervals were similar between sex and ethnic groups, but lower in younger participants. Lipid profile measurement with Cobas\u00ae 6000 is a reliable and accurate analysis in our context. Specific reference intervals must be used in African population, with further studies need for different age subgroups.<\/jats:p>","DOI":"10.1002\/lipd.12350","type":"journal-article","created":{"date-parts":[[2022,6,3]],"date-time":"2022-06-03T23:18:40Z","timestamp":1654298320000},"page":"233-240","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Validation of lipid profile measurement methods and establishment of reference values in a sub\u2010Saharan African population"],"prefix":"10.1002","volume":"57","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7880-7638","authenticated-orcid":false,"given":"C\u00e9cile Danielle","family":"Tang Tsana","sequence":"first","affiliation":[{"name":"Faculty of Medicine and Biomedical Sciences The University of Yaound\u00e9 I  Yaound\u00e9 Cameroon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6192-9580","authenticated-orcid":false,"given":"Guy","family":"Sadeu Wafeu","sequence":"additional","affiliation":[{"name":"Centre for Research on Filariasis and Other Tropical Diseases (CRFilMT)  Yaound\u00e9 Cameroon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1984-103X","authenticated-orcid":false,"given":"Vicky Jocelyne","family":"Ama Moor","sequence":"additional","affiliation":[{"name":"Faculty of Medicine and Biomedical Sciences The University of Yaound\u00e9 I  Yaound\u00e9 Cameroon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bertille Elodie","family":"Edinga\u2010Melengue","sequence":"additional","affiliation":[{"name":"Department of Biochemistry Centre Pasteur of Cameroon  Yaound\u00e9 Cameroon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4353-2352","authenticated-orcid":false,"given":"Jan Ren\u00e9","family":"Nkeck","sequence":"additional","affiliation":[{"name":"Faculty of Medicine and Biomedical Sciences The University of Yaound\u00e9 I  Yaound\u00e9 Cameroon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3847-3490","authenticated-orcid":false,"given":"Georges","family":"Nguefack\u2010Tsague","sequence":"additional","affiliation":[{"name":"Faculty of Medicine and Biomedical Sciences The University of Yaound\u00e9 I  Yaound\u00e9 Cameroon"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,6,3]]},"reference":[{"key":"e_1_2_13_2_1","unstructured":"Abbott.The accuracy and reproductibility of a rapid fingerstick method for measuring a complete lipid profile is comparable to clinical diagnosis laboratory methods. [Internet].2019. Available from:https:\/\/content.veeabb.com\/1d09429b\u20108373\u2010419f\u20108f1a\u2010d28f9586863a\/0d38154c\u2010d860\u20104778\u20108242\u20102ae3a5b57916\/0d38154c\u2010d860\u20104778\u20108242\u20102ae3a5b57916_source__v.pdf"},{"key":"e_1_2_13_3_1","unstructured":"Asqualab\u2010accueil\u2010infos.Asqualab\u2010accueil\u2010infos [Internet]. [cited 2021 Jul 8]. Available from:https:\/\/www.asqualab.com\/"},{"key":"e_1_2_13_4_1","first-page":"60","volume-title":"Defining, establishing and verifying reference intervals in the clinical laboratory: approved guideline","author":"Clinical and Laboratory Standards Institute","year":"2010"},{"key":"e_1_2_13_5_1","unstructured":"Cobas Lipid Panel.Cobas Lipid Panel\u2010CHOL\u2010TRIGL\u2010HDL\u2010LDL [Internet]. Roche Diagnostics;2015[cited 2019 Nov 1]. 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