{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T01:16:27Z","timestamp":1783386987178,"version":"3.54.6"},"reference-count":31,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,7,6]],"date-time":"2026-07-06T00:00:00Z","timestamp":1783296000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,7,6]],"date-time":"2026-07-06T00:00:00Z","timestamp":1783296000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100005703","name":"Akdeniz \u00dcniversitesi","doi-asserted-by":"publisher","award":["TYL\u20102025\u20106830"],"award-info":[{"award-number":["TYL\u20102025\u20106830"]}],"id":[{"id":"10.13039\/501100005703","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004410","name":"T\u00fcrkiye Bilimsel ve Teknolojik Ara\u015ft\u0131rma Kurumu","doi-asserted-by":"publisher","award":["TBTK\u20100082\u20105184"],"award-info":[{"award-number":["TBTK\u20100082\u20105184"]}],"id":[{"id":"10.13039\/501100004410","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["aocs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Lipids"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Sphingolipid metabolism, particularly the balance between pro\u2010apoptotic ceramide and pro\u2010survival sphingosine\u20101\u2010phosphate (S1P), has been associated with breast cancer progression. The aim of the present study was to examine whether ceramide synthase 2 (CerS2) overexpression is associated with changes in increased palmitoyl\u2010CoA (PCA) based ceramide buildup and cell fate changes in MCF\u20107 breast cancer cells, a hormone receptor\u2013positive model. CERS2 plasmid transfected cells were treated with PCA and\/or the CerS inhibitor, fumonisin B1 (FB1). Cell viability, the proliferation marker proliferating cell nuclear antigen (PCNA), apoptosis (TUNEL and cleaved caspase\u20103), and sphingolipid profiles were evaluated. High\u2010dose PCA (100\u2009\u03bcM) significantly reduced MTT signal and PCNA expression and increased apoptotic markers, with stronger effects in CerS2\u2010overexpressing cells. Across short\u2010 and longer\u2010term exposures, the response pattern was concentration\u2010 and time\u2010responsive but not uniformly monotonic. Lipidomic analysis revealed that PCA and CerS2 overexpression increased C16 ceramide and very\u2010long\u2010chain ceramides (C22\u2013C24), respectively. FB1 decreased the level of ceramide, elevated S1P and partly counteracted PCA\u2010induced cytotoxicity. FB1 pre\u2010treatment which was applied sequentially restricted but did not eliminate apoptosis. These findings indicate that CerS2 overexpression reshapes the cellular response to substrate\u2010driven sphingolipid stress by altering acyl\u2010chain\u2013specific ceramide composition and the balance between ceramide\u2010 and S1P\u2010associated signaling. Rather than reflecting isolated pathway effects, the results support a context\u2010dependent role for CerS2 in modulating apoptotic sensitivity in MCF\u20107 breast cancer cells.<\/jats:p>","DOI":"10.1002\/lipd.70071","type":"journal-article","created":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T01:02:02Z","timestamp":1783386122000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["<scp>CerS2<\/scp>\n                    Overexpression Enhances Palmitoyl\u2010\n                    <scp>CoA<\/scp>\n                    \u2013Induced Cytotoxicity and Alters Ceramide Species Composition in Breast Cancer Cells"],"prefix":"10.1002","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6590-0681","authenticated-orcid":false,"given":"Senem Ece","family":"\u00dcner","sequence":"first","affiliation":[{"name":"Department of Gene and Cell Therapy, Faculty of Medicine Akdeniz University  Antalya 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