{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T04:51:06Z","timestamp":1773291066079,"version":"3.50.1"},"reference-count":33,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2002,3,25]],"date-time":"2002-03-25T00:00:00Z","timestamp":1017014400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Lasers Surg Med"],"published-print":{"date-parts":[[2002,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background and Objective<\/jats:title><jats:p>Thrombus formation is initiated by adhesion of the platelet receptor, glycoprotein (GP) Ib\u2010IX\u2010V complex, to its adhesive ligand, von Willebrand factor (vWf), in the subendothelium or plasma. The vWf\u2010binding domain of GP Ib\u2010IX\u2010V is in the GP Ib\u03b1 subunit of the complex and contains a leucine\u2010rich repeat region. The adhesion of different leucine\u2010rich repeats was studied using optical tweezers in order to determine which ones were critical for the vWf\/GP Ib\u03b1 interaction.<\/jats:p><\/jats:sec><jats:sec><jats:title>Study Design\/Materials and Methods<\/jats:title><jats:p>Canine GP Ib\u03b1 does not normally bind to human vWf, and thus canine\u2010human GP Ib\u03b1 chimeras were constructed by sequentially replacing human GP Ib\u03b1 structural regions with their canine counterparts. Chinese hamster ovary (CHO) cells, which are frequently used to express platelet GP complexes, were transfected with the chimeric proteins. Optical tweezers (\u03bb\u2009=\u2009830 nm) were used to investigate bond strengths between vWf and different GP Ib\u03b1 canine\u2010human chimeras. Since vWf does not bind GP Ib\u03b1 without high shear stress, the compounds botrocetin and ristocetin were used to induce binding between human vWf and the chimeras.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>All human\u2010canine GP Ib\u03b1 chimeras bound to vWf in the presence of botrocetin. Replacement of the N\u2010terminal flanking sequence and the first leucine\u2010rich repeat resulted in lower GP Ib\u03b1\/vWf bond strengths than the wild\u2010type human GP Ib\u03b1\/vWf bond strength (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05). Chimeras lacking the second leucine\u2010rich repeat did not adhere to vWf with ristocetin acting as modulator.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The N\u2010terminal flanking sequence and the first leucine\u2010rich repeat of GP Ib\u03b1 were found to be important but not necessary for GP Ib\u03b1 to adhere to vWf. The second leucine\u2010rich repeat was found to be critical for GP Ib\u03b1 to bind vWf and could potentially be used in the development of a novel recombinant anti\u2010thrombotic drugs. Lasers Surg. Med. 30:306\u2013312, 2002. \u00a9 2002 Wiley\u2010Liss, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/lsm.10044","type":"journal-article","created":{"date-parts":[[2002,8,25]],"date-time":"2002-08-25T23:44:07Z","timestamp":1030319047000},"page":"306-312","source":"Crossref","is-referenced-by-count":12,"title":["Measurement of the binding forces between von Willebrand factor and variants of platelet glycoprotein Ib\u03b1 using optical tweezers"],"prefix":"10.1002","volume":"30","author":[{"given":"Maneesh","family":"Arya","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jos\u00e9 A.","family":"L\u00f3pez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gabriel M.","family":"Romo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jing\u2010Fei","family":"Dong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Larry V.","family":"McIntire","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joel L.","family":"Moake","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bahman","family":"Anvari","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2002,3,25]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1097\/00001721-199402000-00013"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1055\/s-0038-1642663"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI119195"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj3060453"},{"key":"e_1_2_1_6_2","doi-asserted-by":"crossref","first-page":"18172","DOI":"10.1016\/S0021-9258(18)55251-0","article-title":"Identification of discontinuous von Willebrand factor sequences involved in complex formation with botrocetin. 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