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To verify whether 5\u2010aminolevulinic acid (ALA)\u2010mediated PDT is effective in MDR cells, we studied the protoporphyrin IX (PpIX) content, intracellular localization, and phototoxicity in human breast cancer cells MCF\u20107 and derived MDR subline, MCF\u20107\/ADR.<\/jats:p><\/jats:sec><jats:sec><jats:title>Study Design\/Materials and Methods<\/jats:title><jats:p>The fluorescence kinetics of ALA\u2010induced PpIX was evaluated by spectrofluorometer. The phototoxicity of MCF\u20107 and MCF\u20107\/ADR cells was determined by tetrazolium (MTT) assays and clonogenic assay. Furthermore, Annexin V and propidium iodide (PI) binding assays were performed to analyze the characteristics of cell death after ALA\u2013PDT.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>MCF\u20107\/ADR accumulated a lower level of PpIX as compared to parental MCF\u20107 cells. Significant phototoxicity was observed in MCF\u20107 and increased in a fluence\u2010dependent manner with LD<jats:sub>50<\/jats:sub> around 8 J\/cm<jats:sup>2<\/jats:sup>. Compared to its parental counterpart, MCF\u20107\/ADR cells were less sensitive to ALA photodynamic treatment and PDT\u2010induced cytotoxicity did not increase in a dose responsive manner as the concentration of ALA increased or the fluence of light increased. ALA\u2013PDT was less effective for MCF\u20107\/ADR cells than MCF\u20107 cells even under the condition when these two cell lines contained the similar amounts of PpIX.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>These results indicate that, except for the MDR related characteristics, MCF\u20107\/ADR cells might possess intrinsic mechanisms that render them less sensitive to ALA\u2013PDT induced phototoxicity. Lasers Surg. Med. 34:62\u201372, 2004. \u00a9 2004 Wiley\u2010Liss, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/lsm.10246","type":"journal-article","created":{"date-parts":[[2004,1,28]],"date-time":"2004-01-28T11:00:34Z","timestamp":1075287634000},"page":"62-72","source":"Crossref","is-referenced-by-count":56,"title":["Effect of 5\u2010aminolevulinic acid\u2010mediated photodynamic therapy on MCF\u20107 and MCF\u20107\/ADR 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