{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T07:34:29Z","timestamp":1777620869016,"version":"3.51.4"},"reference-count":35,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2009,9,10]],"date-time":"2009-09-10T00:00:00Z","timestamp":1252540800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Lasers Surg Med"],"published-print":{"date-parts":[[2009,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background and Objectives<\/jats:title><jats:p>Disruption of the vocal fold extracellular matrix (ECM) can induce a profound and refractory dysphonia. Pulsed dye laser (PDL) irradiation has shown early promise as a treatment modality for disordered ECM in patients with chronic vocal fold scar; however, there are limited data addressing the mechanism by which this laser energy might induce cellular and extracellular changes in vocal fold tissues. In this study, we examined the inflammatory and ECM modulating effects of PDL irradiation on normal vocal fold tissues and cultured vocal fold fibroblasts (VFFs).<\/jats:p><\/jats:sec><jats:sec><jats:title>Study Design\/Materials and Methods<\/jats:title><jats:p>We evaluated the effects of 585\u2009nm PDL irradiation on inflammatory cytokine and collagen\/collagenase gene transcription in normal rat vocal folds in vivo (3\u2013168\u2009hours following delivery of \u223c39.46\u2009J\/cm<jats:sup>2<\/jats:sup> fluence) and VFFs in vitro (3\u201372\u2009hours following delivery of 4.82 or 9.64\u2009J\/cm<jats:sup>2<\/jats:sup> fluence). We also examined morphological vocal fold tissue changes 3\u2009hours, 1 week, and 1 month post\u2010irradiation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>PDL irradiation altered inflammatory cytokine and procollagen\/collagenase expression at the transcript level, both in vitro and in vivo. Additionally, PDL irradiation induced an inflammatory repair process in vivo that was completed by 1 month with preservation of normal tissue morphology.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>PDL irradiation can modulate ECM turnover in phenotypically normal vocal folds. Additional work is required to determine if these findings extend to disordered ECM, such as is seen in vocal fold scar. Lasers Surg. Med. 41:585\u2013594, 2009. \u00a9 2009 Wiley\u2010Liss, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/lsm.20839","type":"journal-article","created":{"date-parts":[[2009,9,10]],"date-time":"2009-09-10T19:18:26Z","timestamp":1252610306000},"page":"585-594","source":"Crossref","is-referenced-by-count":26,"title":["Pulsed dye laser\u2010induced inflammatory response and extracellular matrix turnover in rat vocal folds and vocal fold fibroblasts"],"prefix":"10.1002","volume":"41","author":[{"given":"Ya","family":"Lin","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masaru","family":"Yamashita","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jingxian","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Changying","family":"Ling","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nathan 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