{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T11:41:05Z","timestamp":1786966865765,"version":"build-2736575974"},"reference-count":52,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2010,10,25]],"date-time":"2010-10-25T00:00:00Z","timestamp":1287964800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Lasers Surg Med"],"published-print":{"date-parts":[[2010,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background and Objective<\/jats:title><jats:p>Post\u2010traumatic nerve repair is still a challenge for rehabilitation. It is particularly important to develop clinical protocols to enhance nerve regeneration. The present study investigated the effects of 660 and 780\u2009nm low\u2010level laser therapy (LLLT) using different energy densities (10, 60, and 120\u2009J\/cm<jats:sup>2<\/jats:sup>) on neuromuscular and functional recovery as well as on matrix metalloproteinase (MMP) activity after crush injury in rat sciatic nerve.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>Rats received transcutaneous LLLT irradiation at the lesion site for 10 consecutive days post\u2010injury and were sacrificed 28 days after injury. Both the sciatic nerve and tibialis anterior muscles were analyzed. Nerve analyses consisted of histology (light microscopy) and measurements of myelin, axon, and nerve fiber cross\u2010sectional area (CSA). S\u2010100 labeling was used to identify myelin sheath and Schwann cells. Muscle fiber CSA and zymography were carried out to assess the degree of muscle atrophy and MMP activity, respectively. Statistical significance was set at 5% (<jats:italic>P<\/jats:italic>\u22640.05).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Six hundred sixty nanometer LLLT either using 10 or 60\u2009J\/cm<jats:sup>2<\/jats:sup> restored muscle fiber, myelin and nerve fiber CSA compared to the normal group (N). Furthermore, it increased MMP\u20102 activity in nerve and decreased MMP\u20102 activity in muscle and MMP\u20109 activity in nerve. In contrast, 780\u2009nm LLLT using 10\u2009J\/cm<jats:sup>2<\/jats:sup> decreased MMP\u20109 activity in nerve compared to the crush group (CR) and N; it also restored normal levels of myelin and nerve fiber CSA. Both 60 and 120\u2009J\/cm<jats:sup>2<\/jats:sup> decreased MMP\u20102 activity in muscle compared to CR and N. 780\u2009nm did not prevent muscle fiber atrophy. Functional recovery in the irradiated groups did not differ from the non\u2010irradiated CR.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Data suggest that 660\u2009nm LLLT with low (10\u2009J\/cm<jats:sup>2<\/jats:sup>) or moderate (60\u2009J\/cm<jats:sup>2<\/jats:sup>) energy densities is able to accelerate neuromuscular recovery after nerve crush injury in rats. Lasers Surg. Med. 42:833\u2013842, 2010 \u00a9 2010 Wiley\u2010Liss, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/lsm.20978","type":"journal-article","created":{"date-parts":[[2010,10,25]],"date-time":"2010-10-25T20:12:10Z","timestamp":1288037530000},"page":"833-842","source":"Crossref","is-referenced-by-count":65,"title":["Effects of 660 and 780\u2009nm low\u2010level laser therapy on neuromuscular recovery after crush injury in rat sciatic nerve"],"prefix":"10.1002","volume":"42","author":[{"given":"Davilene","family":"Gigo\u2010Benato","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thiago Luiz","family":"Russo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erika Harumi","family":"Tanaka","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L\u00edvia","family":"Assis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tania F\u00e1tima","family":"Salvini","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nivaldo Antonio","family":"Parizotto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2010,10,25]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1002\/mus.20305"},{"key":"e_1_2_6_3_2","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1016\/S0749-0712(21)00503-5","article-title":"Techniques for nerve grafting","volume":"16","author":"Millesi H","year":"2000","journal-title":"Hand Clinics"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/ar.1120"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1002\/mus.20624"},{"key":"e_1_2_6_6_2","volume-title":"Nerve Injury and Repair","author":"Lundborg G","year":"2005"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00080.2004"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1152\/physiolgenomics.00210.2004"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-510X(96)00219-5"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1002\/mus.20054"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainres.2006.09.016"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2990.2005.00676.x"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1002\/jnr.10783"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1093\/jnen\/60.1.85"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1002\/mus.20145"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1097\/00001756-199909090-00018"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1002\/mus.10529"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2990.1998.00118.x"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-4598(200004)23:4<580::AID-MUS18>3.0.CO;2-4"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0404.2002.1o104.x"},{"key":"e_1_2_6_21_2","doi-asserted-by":"publisher","DOI":"10.1002\/lsm.20757"},{"key":"e_1_2_6_22_2","doi-asserted-by":"publisher","DOI":"10.1055\/s-2001-17755"},{"key":"e_1_2_6_23_2","doi-asserted-by":"publisher","DOI":"10.1089\/neu.2004.21.1652"},{"key":"e_1_2_6_24_2","doi-asserted-by":"publisher","DOI":"10.1097\/00006534-198901000-00024"},{"key":"e_1_2_6_25_2","doi-asserted-by":"publisher","DOI":"10.1002\/micr.10199"},{"key":"e_1_2_6_26_2","doi-asserted-by":"publisher","DOI":"10.1002\/mus.20985"},{"key":"e_1_2_6_27_2","article-title":"Stretching and electrical stimulation regulate the metalloproteinase\u20102 in rat denervated skeletal muscle","author":"Peviani SM","year":"2009","journal-title":"Neurol Res"},{"key":"e_1_2_6_28_2","doi-asserted-by":"publisher","DOI":"10.1179\/016164104225013914"},{"key":"e_1_2_6_29_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01402391"},{"key":"e_1_2_6_30_2","doi-asserted-by":"crossref","first-page":"843","DOI":"10.1227\/00006123-198706000-00004","article-title":"Stimulatory effect of He\u2013Ne low dose laser on injured sciatic nerves of rats","volume":"20","author":"Rochkind S","year":"1987","journal-title":"Neurosurgery"},{"key":"e_1_2_6_31_2","doi-asserted-by":"publisher","DOI":"10.1002\/lsm.1900070512"},{"key":"e_1_2_6_32_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01406620"},{"key":"e_1_2_6_33_2","doi-asserted-by":"publisher","DOI":"10.1002\/lsm.1900090214"},{"key":"e_1_2_6_34_2","doi-asserted-by":"publisher","DOI":"10.1097\/00007632-199001000-00003"},{"key":"e_1_2_6_35_2","doi-asserted-by":"publisher","DOI":"10.1080\/01616412.1992.11740003"},{"key":"e_1_2_6_36_2","doi-asserted-by":"publisher","DOI":"10.1002\/lsm.1041"},{"key":"e_1_2_6_37_2","doi-asserted-by":"publisher","DOI":"10.1089\/pho.2007.2093"},{"key":"e_1_2_6_38_2","doi-asserted-by":"publisher","DOI":"10.1089\/pho.2007.2076"},{"key":"e_1_2_6_39_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0074-7742(09)87025-6"},{"key":"e_1_2_6_40_2","doi-asserted-by":"publisher","DOI":"10.1089\/pho.2009.9955"},{"key":"e_1_2_6_41_2","first-page":"380","volume-title":"Laser Therapy: Clinical Practice and Scientific Background","author":"Tun\u00e9r J","year":"2003"},{"key":"e_1_2_6_42_2","doi-asserted-by":"publisher","DOI":"10.2519\/jospt.1992.15.5.209"},{"key":"e_1_2_6_43_2","doi-asserted-by":"publisher","DOI":"10.1002\/lsm.1900130113"},{"key":"e_1_2_6_44_2","volume-title":"Color Atlas of Nerve Biopsy Pathology","author":"Shin OHJ","year":"2005"},{"key":"e_1_2_6_45_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.184.6.2311"},{"key":"e_1_2_6_46_2","doi-asserted-by":"publisher","DOI":"10.1097\/00001756-200011090-00003"},{"key":"e_1_2_6_47_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.22-17-07526.2002"},{"key":"e_1_2_6_48_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0722.1995.tb00030.x"},{"key":"e_1_2_6_49_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10103-003-0258-6"},{"key":"e_1_2_6_50_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-3940(03)00354-9"},{"key":"e_1_2_6_51_2","first-page":"86","article-title":"An alternative video footprint analysis to assess functional loss following injury to the rat sciatic nerve","volume":"44","author":"Bervar M","year":"2002","journal-title":"Acta Chir Plast"},{"key":"e_1_2_6_52_2","first-page":"1","article-title":"Walking track analysis: An assessment method for functional recovery after sciatic nerve injury in the rat","volume":"68","author":"Sarikciouglu L","year":"2009","journal-title":"Folia Morphol (Warsz)"},{"key":"e_1_2_6_53_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0074-7742(09)87007-4"}],"container-title":["Lasers in Surgery and Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Flsm.20978","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/lsm.20978","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,9]],"date-time":"2023-10-09T16:44:45Z","timestamp":1696869885000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/lsm.20978"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,10,25]]},"references-count":52,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2010,11]]}},"alternative-id":["10.1002\/lsm.20978"],"URL":"https:\/\/doi.org\/10.1002\/lsm.20978","archive":["Portico"],"relation":{},"ISSN":["0196-8092","1096-9101"],"issn-type":[{"value":"0196-8092","type":"print"},{"value":"1096-9101","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,10,25]]}}}