{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,20]],"date-time":"2026-04-20T22:59:05Z","timestamp":1776725945321,"version":"3.51.2"},"reference-count":26,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2011,7,14]],"date-time":"2011-07-14T00:00:00Z","timestamp":1310601600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100002602","name":"American Society for Laser Medicine and Surgery","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100002602","id-type":"DOI","asserted-by":"crossref"}]},{"name":"WangKuanCheng Foundation of the School of Medicine"},{"DOI":"10.13039\/501100004921","name":"Shanghai Jiao Tong University","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100004921","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Lasers Surg Med"],"published-print":{"date-parts":[[2011,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background and Objective<\/jats:title><jats:p>Amniotic membrane transplantation (AMT) sealed with sutures has been routinely used to treat ocular surface defects (OSD). However, the sutures used to secure the graft on the cornea cause additional injury, infection, and scarring. A new light\u2010activated technique, called photochemical tissue bonding (PTB), has been developed for securing amniotic membrane (AM) over the corneal surface. The purpose of this study was to compare PTB versus traditional sutures in AMT for repair of OSD.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>An OSD was created in the left eye of 40 rabbits. The eyes were randomized into two repair groups: AMT using sutures and AMT using PTB with 0.1% Rose Bengal (RB) and 532\u2009nm laser at 0.4\u2009W\/cm<jats:sup>2<\/jats:sup> for 200\u2009seconds. Eyes were examined for re\u2010epithelialization, inflammation, neovascularization, and scarring histologically and biochemically on postoperative days 1, 3, 14, and 28.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>PTB strongly bonded AM over corneal defects. Corneal re\u2010epithelialization did not differ significantly between the suture and PTB groups. Histology, immunohistology and Western blotting revealed that the numbers of inflammatory cells and the level of tumor necrosis factor\u2010alpha in the PTB group were dramatically lower than those in the suture group on postoperative day 3. Many fewer neo\u2010vessels were present in the PTB group (2.91\u2009\u00b1\u20091.00) compared to the suture group (4.33\u2009\u00b1\u20091.15) at day 28 (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05). The collagen fibers in the PTB group were well organized and orientated as assessed by second harmonic generation microscopy, suggesting that PTB treatment led to less corneal scarring.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>PTB is a superior method for securing AM over OSD with improved wound healing compared to sutures. Lasers Surg. Med. 43:481\u2013489, 2011. \u00a9 2011 Wiley\u2010Liss, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/lsm.21083","type":"journal-article","created":{"date-parts":[[2011,7,14]],"date-time":"2011-07-14T19:48:54Z","timestamp":1310672934000},"page":"481-489","source":"Crossref","is-referenced-by-count":23,"title":["A light\u2010activated method for repair of corneal surface defects"],"prefix":"10.1002","volume":"43","author":[{"given":"Ying","family":"Wang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Irene E.","family":"Kochevar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert W.","family":"Redmond","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Min","family":"Yao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2011,7,14]]},"reference":[{"issue":"3","key":"e_1_2_8_2_2","first-page":"214","article-title":"Corneal blindness: A global perspective","volume":"79","author":"Whitcher JP","year":"2001","journal-title":"Bull World Health Organ"},{"issue":"5","key":"e_1_2_8_3_2","doi-asserted-by":"crossref","first-page":"576","DOI":"10.47102\/annals-acadmedsg.V33N5p576","article-title":"Ocular surface stem cells and disease: Current concepts and clinical applications","volume":"33","author":"Ang LP","year":"2004","journal-title":"Ann Acad Med Singapore"},{"key":"e_1_2_8_4_2","doi-asserted-by":"publisher","DOI":"10.4103\/0301-4738.33036"},{"key":"e_1_2_8_5_2","doi-asserted-by":"publisher","DOI":"10.1097\/00003226-199509000-00006"},{"key":"e_1_2_8_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.survophthal.2003.10.004"},{"key":"e_1_2_8_7_2","doi-asserted-by":"publisher","DOI":"10.1097\/00003226-200111000-00008"},{"key":"e_1_2_8_8_2","doi-asserted-by":"publisher","DOI":"10.1006\/jsre.2002.6516"},{"key":"e_1_2_8_9_2","doi-asserted-by":"publisher","DOI":"10.1002\/lsm.20221"},{"key":"e_1_2_8_10_2","doi-asserted-by":"publisher","DOI":"10.1002\/lsm.20548"},{"key":"e_1_2_8_11_2","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.03-1066"},{"key":"e_1_2_8_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jss.2008.01.025"},{"issue":"10","key":"e_1_2_8_13_2","first-page":"1668","article-title":"Biosynthetic responses of the rabbit cornea to a keratectomy wound","volume":"28","author":"Zieske JD","year":"1987","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"e_1_2_8_14_2","doi-asserted-by":"publisher","DOI":"10.1001\/archopht.1992.01080130014004"},{"key":"e_1_2_8_15_2","doi-asserted-by":"publisher","DOI":"10.1002\/jbio.200810028"},{"key":"e_1_2_8_16_2","doi-asserted-by":"publisher","DOI":"10.4103\/0301-4738.55079"},{"key":"e_1_2_8_17_2","doi-asserted-by":"publisher","DOI":"10.1097\/01.ico.0000183493.00884.8f"},{"key":"e_1_2_8_18_2","doi-asserted-by":"publisher","DOI":"10.1097\/ICO.0b013e318158b47f"},{"key":"e_1_2_8_19_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1463-5216.2001.00195.x"},{"key":"e_1_2_8_20_2","doi-asserted-by":"publisher","DOI":"10.1097\/00003226-200307000-00003"},{"key":"e_1_2_8_21_2","doi-asserted-by":"publisher","DOI":"10.1097\/ICO.0b013e3181506129"},{"key":"e_1_2_8_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.thromres.2007.12.027"},{"key":"e_1_2_8_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0886-3350(87)80073-1"},{"key":"e_1_2_8_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcrs.2007.04.013"},{"key":"e_1_2_8_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2008.06.007"},{"key":"e_1_2_8_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1011-1344(96)07300-9"},{"key":"e_1_2_8_27_2","doi-asserted-by":"publisher","DOI":"10.1039\/b712496a"}],"container-title":["Lasers in Surgery and Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Flsm.21083","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Flsm.21083","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/lsm.21083","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,14]],"date-time":"2023-10-14T14:39:29Z","timestamp":1697294369000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/lsm.21083"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,7,14]]},"references-count":26,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2011,8]]}},"alternative-id":["10.1002\/lsm.21083"],"URL":"https:\/\/doi.org\/10.1002\/lsm.21083","archive":["Portico"],"relation":{},"ISSN":["0196-8092","1096-9101"],"issn-type":[{"value":"0196-8092","type":"print"},{"value":"1096-9101","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011,7,14]]}}}