{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:50:03Z","timestamp":1786978203845,"version":"build-2736575974"},"reference-count":43,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2011,10,17]],"date-time":"2011-10-17T00:00:00Z","timestamp":1318809600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Funda\u00e7\u00e3o de Amparo a Pesquisa do Estado de S\u00e3o Paulo","award":["FAPESP 2008\/03994-9; 2008\/00601-6"],"award-info":[{"award-number":["FAPESP 2008\/03994-9; 2008\/00601-6"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Lasers Surg Med"],"published-print":{"date-parts":[[2011,11]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background and Objective<\/jats:title>\n                    <jats:p>\n                      The resistance of\n                      <jats:italic>Candida<\/jats:italic>\n                      species to antifungals represents a major challenge for therapeutic and prophylactic strategies. This study evaluated photodynamic therapy (PDT) mediated by Curcumin (CUR) against clinical isolates of\n                      <jats:italic>C. albicans, C. tropicalis,<\/jats:italic>\n                      and\n                      <jats:italic>C. glabrata<\/jats:italic>\n                      , both in planktonic and biofilm forms.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Study Design\/Materials and Methods<\/jats:title>\n                    <jats:p>\n                      Suspensions of\n                      <jats:italic>Candida<\/jats:italic>\n                      were treated with three CUR concentrations and exposed to four LED fluences. The protocol that showed the best outcomes for inactivation of the planktonic phase was selected to be evaluated against\n                      <jats:italic>Candida<\/jats:italic>\n                      biofilms. In addition, two higher CUR concentrations were tested. The metabolic activity of biofilms was evaluated by means of XTT reduction assay and the biofilm biomass was evaluated using crystal violet (CV) staining assay. Data were analyzed in a mixed model nested ANOVA, Wilcoxon's nonparametric tests, and the Kruskal\u2013Wallis test (\n                      <jats:italic>\u03b1<\/jats:italic>\n                      \u2009=\u20095%).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The use of CUR in association with light was able to promote a significant antifungal effect against the planktonic form of the yeasts. When using 40\u2009\u00b5M of CUR, the metabolic activity of\n                      <jats:italic>C. albicans<\/jats:italic>\n                      ,\n                      <jats:italic>C. glabrata<\/jats:italic>\n                      , and\n                      <jats:italic>C. tropicalis<\/jats:italic>\n                      biofilms was reduced by 85%, 85%, and 73%, respectively, at 18\u2009J\/cm\n                      <jats:sup>2<\/jats:sup>\n                      . CUR\u2010mediated PDT also decreased the biofilm biomass of all species evaluated. In addition, CV staining showed that\n                      <jats:italic>C. albicans<\/jats:italic>\n                      isolates were strong biofilm\u2010forming strains, when compared with\n                      <jats:italic>C. glabrata<\/jats:italic>\n                      and\n                      <jats:italic>C. tropicalis<\/jats:italic>\n                      isolates.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      The results from the present investigation showed that low CUR concentrations can be highly effective for inactivating\n                      <jats:italic>Candida<\/jats:italic>\n                      isolates when associated with light excitation. Lasers Surg. Med. 43:927\u2010934, 2011. \u00a9 2011 Wiley Periodicals, Inc.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/lsm.21110","type":"journal-article","created":{"date-parts":[[2011,10,17]],"date-time":"2011-10-17T16:30:12Z","timestamp":1318869012000},"page":"927-934","source":"Crossref","is-referenced-by-count":125,"title":["Susceptibility of clinical isolates of\n                    <i>Candida<\/i>\n                    to photodynamic effects of curcumin"],"prefix":"10.1002","volume":"43","author":[{"given":"L\u00edvia N.","family":"Dovigo","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana C.","family":"Pavarina","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juliana C.","family":"Carmello","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana L.","family":"Machado","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Iguatemy L.","family":"Brunetti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vanderlei S.","family":"Bagnato","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2011,10,17]]},"reference":[{"key":"e_1_2_6_2_2","volume-title":"Candida and candidosis: A review and bibliography","author":"Odds FC","year":"1988"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10103-011-0912-3"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1177\/00220345950740050301"},{"key":"e_1_2_6_5_2","first-page":"66","volume-title":"Oral candidosis","author":"Samaranayake LP","year":"1990"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1177\/10454411940050020101"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1053\/jhin.2001.1151"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1093\/clinids\/20.1.115"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0360-3016(03)00415-2"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.prosdent.2004.11.015"},{"key":"e_1_2_6_11_2","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1128\/jcm.32.1.59-64.1994","article-title":"Variations in fluconazole susceptibility and electrophoretic karyotype among oral isolates of Candida albicans from patients with AIDS and oral candidiasis","volume":"32","author":"Pfaller MA","year":"1994","journal-title":"J Clin Microbiol"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.archoralbio.2009.08.002"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1039\/b311900a"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.micres.2007.08.001"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.48.6.2000-2006.2004"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0507.2009.01769.x"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2736(92)90117-5"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2133.2001.04013.x"},{"key":"e_1_2_6_19_2","first-page":"345","article-title":"The photodynamic inactivation of the yeast Candida guilliermondii in the presence of photodithazine","volume":"71","author":"Strackhovskaya MG","year":"2002","journal-title":"Microbiology"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biocel.2007.07.014"},{"key":"e_1_2_6_21_2","doi-asserted-by":"publisher","DOI":"10.1039\/B9PP00154A"},{"key":"e_1_2_6_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tripleo.2009.10.006"},{"key":"e_1_2_6_23_2","doi-asserted-by":"publisher","DOI":"10.1017\/S0007114509993667"},{"key":"e_1_2_6_24_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1751-1097.2011.00937.x"},{"key":"e_1_2_6_25_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1751-1097.1994.tb05036.x"},{"key":"e_1_2_6_26_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1751-1097.1994.tb05037.x"},{"key":"e_1_2_6_27_2","first-page":"666","article-title":"Photokilling of bacteria by curcumin in different aqueous preparations. 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