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We evaluate the potential of these changes in fluorescence to predict a diagnosis of malignancy and to estimate the risk of developing ovarian cancer.<\/jats:p><\/jats:sec><jats:sec><jats:title>Study Design\/Materials and Methods<\/jats:title><jats:p>Ovarian biopsies were interrogated over 270\u2013550\u2009nm excitation and fluorescence was collected from 290 to 700\u2009nm. Two hundred forty\u2010nine measurements were performed on 49 IRB\u2010consented patients undergoing oophorectomy. Data are analyzed using parallel factor analysis to determine excitation and emission spectra of the underlying fluorophores that contribute to the total detected fluorescence intensity.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Using multivariate normal distribution fits and cross\u2010validation techniques, sensitivity and specificity of 88% and 93%, respectively, are achieved when classifying malignant samples versus others, while 88% and 80%, respectively, are achieved when classifying normal post\u2010menopausal patients as being either at high\u2010 or low\u2010risk of developing ovarian cancer based on their personal and family history of cancer. Performance of classifying cancer increases when the normal group does not include benign neoplasm and endometriosis samples. Performance of high\u2010 versus low\u2010risk classification decreases when normal samples include both pre\u2010 and post\u2010menopausal women. Excitation over 270\u2013400 and 380\u2013560\u2009nm, respectively, have the best diagnostic performance for cancer detection and risk\u2010status assessment.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Assessing the endogenous fluorescence could be useful in screening women at increased risk of developing ovarian cancer. Lasers Surg. Med. 44:282\u2013295, 2012. \u00a9 2012 Wiley Periodicals, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/lsm.22014","type":"journal-article","created":{"date-parts":[[2012,3,7]],"date-time":"2012-03-07T17:23:48Z","timestamp":1331141028000},"page":"282-295","source":"Crossref","is-referenced-by-count":16,"title":["Parallel factor analysis of ovarian autofluorescence as a cancer diagnostic"],"prefix":"10.1002","volume":"44","author":[{"given":"Ronie","family":"George","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michalis","family":"Michaelides","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Molly 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