{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T15:19:56Z","timestamp":1787066396112,"version":"build-2736575974"},"reference-count":21,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2013,4,8]],"date-time":"2013-04-08T00:00:00Z","timestamp":1365379200000},"content-version":"vor","delay-in-days":7,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"crossref","award":["2010\/11795-6"],"award-info":[{"award-number":["2010\/11795-6"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Lasers Surg Med"],"published-print":{"date-parts":[[2013,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background and Objectives<\/jats:title>\n                    <jats:p>\n                      Peripheral nerve function can be debilitated by different kinds of injury. Low\u2010level laser therapy (LLLT) has been used successfully during rehabilitation to stimulate recovery. The aim of this study was to evaluate the effects of LLLT (660\u2009nm, 60\u2009J\/cm\n                      <jats:sup>2<\/jats:sup>\n                      , 40\u2009mW\/cm\n                      <jats:sup>2<\/jats:sup>\n                      ) on acute sciatic nerve injury.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods<\/jats:title>\n                    <jats:p>Thirty Wistar male rats were divided into three groups: (1) Normal, intact nerves; (2) I3d, crushed nerves evaluated on Day\u20103 post\u2010injury; (3) I\u2009+\u2009L3d, crushed nerves submitted to two sessions of LLLT and investigated at 3 days post\u2010injury. Sciatic nerves were removed and processed for gene expression analysis (real\u2010time PCR) of the pro\u2010inflammatory factors TWEAK, Fn14 and TNF\u2010\u03b1 and extracellular matrix remodeling and axonal growth markers, such as TIMP\u20101, MMP\u20102, and MMP\u20109. Zymography was used to determine levels of MMP\u20102 and MMP\u20109 activity and Western blotting was used to evaluate TNF\u2010\u03b1 protein content. Shapiro\u2013Wilk and Levene's tests were applied to evaluate data normality and homogeneity, respectively. One\u2010way ANOVA followed by Tukey test was used for statistical analysis with a significance level set at 5%.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      An increase in TNF\u2010\u03b1 protein level was found in I\u2009+\u2009L3 compared to Normal and I3d (\n                      <jats:italic>P<\/jats:italic>\n                      \u2009&lt;\u20090.05). Zymography showed an increase in proMMP\u20109 activity, in both I3d and I\u2009+\u2009L3d groups (\n                      <jats:italic>P<\/jats:italic>\n                      \u2009&lt;\u20090.05). The increase was more evident in I\u2009+\u2009L3d (\n                      <jats:italic>P<\/jats:italic>\n                      \u2009=\u20090.02 compared to I3d). Active\u2010MMP\u20109 isoform activity was increased in I\u2009+\u2009L3d compared to Normal and I3d groups (\n                      <jats:italic>P<\/jats:italic>\n                      \u2009&lt;\u20090.05). Furthermore, the activity of active\u2010MMP\u20102 isoform was increased in I3d and I\u2009+\u2009L3 (\n                      <jats:italic>P<\/jats:italic>\n                      \u2009&lt;\u20090.05). An increase in TIMP\u20101 expression was observed in both I3d and I\u2009+\u2009L3d groups (\n                      <jats:italic>P<\/jats:italic>\n                      \u2009&lt;\u20090.05).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>The current study showed that LLLT increased MMPs activity, mainly MMP\u20109, and TNF\u2010\u03b1 protein level during the acute phase of nerve injury, modulating inflammation. Based on these results, it is recommended that LLLT should be started as soon as possible after peripheral nerve injury. Lasers Surg. Med. 9999:XX\u2013XX, 2013. \u00a9 2013 Wiley Periodicals, Inc.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/lsm.22129","type":"journal-article","created":{"date-parts":[[2013,4,8]],"date-time":"2013-04-08T08:31:57Z","timestamp":1365409917000},"page":"246-252","source":"Crossref","is-referenced-by-count":40,"title":["Effect of Low\u2010\n                    <scp>L<\/scp>\n                    evel Laser Therapy (\n                    <scp>LLLT<\/scp>\n                    ) on Acute Neural Recovery and Inflammation\u2010\n                    <scp>R<\/scp>\n                    elated Gene Expression After Crush Injury in Rat Sciatic Nerve"],"prefix":"10.1002","volume":"45","author":[{"given":"Carolina C.","family":"Alc\u00e2ntara","sequence":"first","affiliation":[{"name":"Laboratory of Neurological Physiotherapy Research, Physical Therapy Department, Federal University of S\u00e3o Carlos (UFSCar) S\u00e3o Carlos 13565\u2010905, SP Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Davilene","family":"Gigo\u2010Benato","sequence":"additional","affiliation":[{"name":"Laboratory of Neurological Physiotherapy Research, Physical Therapy Department, Federal University of S\u00e3o Carlos (UFSCar) S\u00e3o Carlos 13565\u2010905, SP Brazil"},{"name":"Skeletal Muscle Plasticity Unit, Physical Therapy Department, Federal University of S\u00e3o Carlos (UFSCar) S\u00e3o Carlos 13565\u2010905, SP Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tania F.","family":"Salvini","sequence":"additional","affiliation":[{"name":"Skeletal Muscle Plasticity Unit, Physical Therapy Department, Federal University of S\u00e3o Carlos (UFSCar) S\u00e3o Carlos 13565\u2010905, SP Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandre L. R.","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Department of Anatomy, Cell Biology, Physiology and Biophysics\u2010Institute of Biology University of Campinas (UNICAMP) Campinas 13083\u2010865, SP Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juanita J.","family":"Anders","sequence":"additional","affiliation":[{"name":"Department of Anatomy, Physiology and Genetics, Uniformed Services University of the Health Sciences, Bethesda Maryland 20814"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thiago L.","family":"Russo","sequence":"additional","affiliation":[{"name":"Laboratory of Neurological Physiotherapy Research, Physical Therapy Department, Federal University of S\u00e3o Carlos (UFSCar) S\u00e3o Carlos 13565\u2010905, SP Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2013,4,8]]},"reference":[{"key":"e_1_2_7_2_1","first-page":"49","volume-title":"Nerve injury and repair: Regeneration, reconstruction and cortical remodeling","author":"Lundborg G","year":"2004"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1065874"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/mus.20985"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.184.6.2311"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/mus.10529"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/jnen\/60.1.85"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2990.1998.00118.x"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1529-8027.2002.02003.x"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12035-007-0024-z"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/mus.20305"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/lsm.20978"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1080\/01616412.1996.11740454"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0074-7742(09)87025-6"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1179\/174313209X459093"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1097\/WCO.0b013e32834c208d"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1186\/1742-2094-8-110"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1186\/1742-2094-8-109"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1159\/000292020"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mcn.2005.10.011"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/jnr.10783"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200909117"}],"container-title":["Lasers in Surgery and Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Flsm.22129","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Flsm.22129","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/lsm.22129","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,30]],"date-time":"2023-10-30T03:28:30Z","timestamp":1698636510000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/lsm.22129"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,4]]},"references-count":21,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2013,4]]}},"alternative-id":["10.1002\/lsm.22129"],"URL":"https:\/\/doi.org\/10.1002\/lsm.22129","archive":["Portico"],"relation":{},"ISSN":["0196-8092","1096-9101"],"issn-type":[{"value":"0196-8092","type":"print"},{"value":"1096-9101","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,4]]}}}