{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,15]],"date-time":"2026-08-15T08:43:42Z","timestamp":1786783422697,"version":"build-2736575974"},"reference-count":43,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2014,4,2]],"date-time":"2014-04-02T00:00:00Z","timestamp":1396396800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Lasers Surg Med"],"published-print":{"date-parts":[[2014,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>The aim of this study was to investigate the effect of bumetanide on peri\u2010tumor edema caused by photodynamic therapy (PDT) of intraparenchymal C6 glioma xenografts.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Seven days after inoculation with C6 cells, rats with MRI\u2010confirmed glioma received hematoporphyrin monomethyl ether (HMME)\u2010mediated PDT, injection of bumetanide or a combination of the two treatments. After treatment, tumor volume, tumor weight, brain water content, microvessel density, expression of NKCC\u20101, Zonula occludens\u20101 (ZO\u20101), and animal survival time were examined.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>In the PDT group, tumor growth was significantly inhibited and survival prolonged. Bumetanide enhanced the efficacy of PDT and reduced PDT\u2010induced peri\u2010tumor edema in the combined PDT\u2009+\u2009bumetanide treatment group where NKCC\u20101 expression in response to PDT was significantly suppressed. ZO\u20101 expression was significantly suppressed in the PDT\u2010only group. This suppression was not observed in the combined PDT\u2009+\u2009bumetanide treatment group.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>PDT, in combination with bumetanide was seen to significantly inhibit the growth of C6 glioma, relieve peri\u2010tumor edema caused by PDT alone and prolong survival. These results suggest that PDT, in combination with bumetanide, may be a useful and promising strategy in the treatment of human glioma. Lasers Surg. Med. 46:422\u2013430, 2014. \u00a9 2014 Wiley Periodicals, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/lsm.22248","type":"journal-article","created":{"date-parts":[[2014,4,2]],"date-time":"2014-04-02T10:15:24Z","timestamp":1396433724000},"page":"422-430","source":"Crossref","is-referenced-by-count":11,"title":["The effect of bumetanide on photodynamic therapy\u2010induced peri\u2010tumor edema of C6 glioma xenografts"],"prefix":"10.1002","volume":"46","author":[{"given":"Xufeng","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Neurosurgery The Second Affiliated Hospital of Harbin Medical University Harbin 150086 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Damin","family":"Cong","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery The Second Affiliated Hospital of Harbin Medical University Harbin 150086 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dawei","family":"Shen","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery Shouguang City People's Hospital Weifang 262700 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"Gao","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery The Second Affiliated Hospital of Harbin Medical University Harbin 150086 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lei","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery The Second Affiliated Hospital of Harbin Medical University Harbin 150086 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shaoshan","family":"Hu","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery The Second Affiliated Hospital of Harbin Medical University Harbin 150086 China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2014,4,2]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2007.13.9337"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/brain\/awh563"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/msj.20223"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/lsm.20445"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1615\/JEnvironPatholToxicolOncol.v26.i2.100"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/lsm.20507"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pdpdt.2011.04.002"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1590\/S0100-879X2011007500052"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pdpdt.2009.01.005"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pdpdt.2006.09.003"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.canlet.2004.07.005"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2005.09.021"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.4149\/neo_2010_01_079"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/lsm.22172"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1020521308985"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1998.274.4.C883"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00251.2006"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.ph.58.030196.003245"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11060-006-9252-8"},{"key":"e_1_2_7_21_1","first-page":"55","article-title":"Growth of human glioblastomas as xenografts in the brains of athymic rats","volume":"16","author":"Ozawa T","year":"2002","journal-title":"In Vivo"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11060-007-9400-9"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2006.07.048"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jneumeth.2008.04.022"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncr.23678"},{"key":"e_1_2_7_26_1","doi-asserted-by":"crossref","first-page":"122","DOI":"10.1152\/ajplegacy.1949.157.1.122","article-title":"Measurement of experimentally induced brain swelling and shrinkage","volume":"157","author":"Elliott KA","year":"1949","journal-title":"Am J Physiol"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00399.2010"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1152\/physiol.00015.2009"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1179\/016164105X25243"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/jcbfm.1994.135"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroscience.2004.06.046"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1097\/CCM.0B013E31816590C4"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2010.06.041"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.00021.2004"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.2170\/physiolsci.RP010806"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.114.2.337"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.1041580115"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1159\/000016367"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-4652(200001)182:1<109::AID-JCP12>3.0.CO;2-A"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1994.267.4.C869"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.HYP.23.6.1022"},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.1041450106"},{"key":"e_1_2_7_43_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00701-004-0422-1"},{"key":"e_1_2_7_44_1","doi-asserted-by":"publisher","DOI":"10.3892\/ol.2012.1060"}],"container-title":["Lasers in Surgery and Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Flsm.22248","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/lsm.22248","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,5]],"date-time":"2023-10-05T10:05:52Z","timestamp":1696500352000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/lsm.22248"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,4,2]]},"references-count":43,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2014,7]]}},"alternative-id":["10.1002\/lsm.22248"],"URL":"https:\/\/doi.org\/10.1002\/lsm.22248","archive":["Portico"],"relation":{},"ISSN":["0196-8092","1096-9101"],"issn-type":[{"value":"0196-8092","type":"print"},{"value":"1096-9101","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,4,2]]}}}