{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T01:22:57Z","timestamp":1791076977556,"version":"4.1.0"},"reference-count":46,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2014,6,7]],"date-time":"2014-06-07T00:00:00Z","timestamp":1402099200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Medical Research Council (ETT) of Hungary","award":["097\/2009"],"award-info":[{"award-number":["097\/2009"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Lasers Surg Med"],"published-print":{"date-parts":[[2014,10]]},"abstract":"<jats:sec><jats:title>Background and Objective<\/jats:title><jats:p>Low level laser therapy (LLLT) has been developed for non\u2010invasive treatment of joint diseases. We have previously shown that LLLT influenced synovial protein expression in rheumatoid arthritis (RA). The aim of this study was to assess the effects of laser irradiation on osteoarthritic (OA) synovial protein expression.<\/jats:p><\/jats:sec><jats:sec><jats:title>Study Design\/Materials and methods<\/jats:title><jats:p>The synovial membrane samples removed from the knees of 6 OA patients were irradiated <jats:italic>ex vivo<\/jats:italic> using near infrared diode laser (807\u2013811\u2009nm; 25\u2009J\/cm<jats:sup>2<\/jats:sup>). An untreated sample taken from the same patient served as control. Synovial protein separation and identification were performed by two\u2010dimensional differential gel electrophoresis and mass spectrometry, respectively.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Eleven proteins showing altered expression due to laser irradiation were identified. There were three patients whose tissue samples demonstrated a significant increase (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05) in mitochondrial heat shock 60\u2009kD protein 1 variant 1. The expression of the other proteins (calpain small subunit 1, tubulin alpha\u20101C and beta 2, vimentin variant 3, annexin A1, annexin A5, cofilin 1, transgelin, and collagen type VI alpha 2 chain precursor) significantly decreased (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05) compared to the control samples.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>A single diode laser irradiation of the synovial samples of patients with osteoarthritis can statistically significantly alter the expression of some proteins <jats:italic>in vitro<\/jats:italic>. These findings provide some more evidence for biological efficacy of LLLT treatment, used for osteoarthritis. Lasers Surg. Med. 46:644\u2013649, 2014. \u00a9 2014 Wiley Periodicals, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/lsm.22268","type":"journal-article","created":{"date-parts":[[2014,6,7]],"date-time":"2014-06-07T10:02:58Z","timestamp":1402135378000},"page":"644-649","source":"Crossref","is-referenced-by-count":8,"title":["Effects of laser treatment on the expression of cytosolic proteins in the synovium of patients with osteoarthritis"],"prefix":"10.1002","volume":"46","author":[{"given":"Kl\u00e1ra","family":"Barab\u00e1s","sequence":"first","affiliation":[{"name":"Outpatient Clinic of Budav\u00e1ri Local Government Unit of Rheumatology Budapest 1122 Hungary"},{"name":"National Institute of Rheumatology and Physiotherapy Budapest 1023 Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00f3zsef","family":"Bakos","sequence":"additional","affiliation":[{"name":"\u201cFr\u00e9d\u00e9ric Joliot\u2010Curie\u201d National Research Institute for Radiobiology and Radiohygiene 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