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This work proposed the discrimination between non\u2010melanoma (basal cell carcinoma, BCC; squamous cell carcinoma, SCC) and pre\u2010cancerous lesions (actinic keratosis, AK) from benign lesions and normal (non\u2010tumor group, NT) tissues, using near\u2010infrared Raman spectroscopy with a Raman probe.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>Prior to surgery, the spectra of suspicious lesions were obtained <jats:italic>in situ<\/jats:italic>. The spectra of adjacent, clinically normal skin were also obtained. Lesions were resectioned and submitted for histopathology. The Raman spectra were measured using a Raman spectrometer (830\u2009nm). Two types of discrimination models were developed to distinguish the different histopathological groups. The principal components analysis discriminant analysis (PCA\/DA) and the partial least squares discriminant analysis (PLS\/DA) were based on Euclidean, quadratic and Mahalanobis distances.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>PCA and PLS spectral vectors showed spectral features of skin constituents, such as lipids (between 1,250\u2009cm<jats:sup>\u22121<\/jats:sup> and 1,300\u2009cm<jats:sup>\u22121<\/jats:sup> and at 1,450\u2009cm<jats:sup>\u22121<\/jats:sup>) and proteins (between 870\u2009cm<jats:sup>\u22121<\/jats:sup> and 940\u2009cm<jats:sup>\u22121<\/jats:sup>, 1,240\u2009cm<jats:sup>\u22121<\/jats:sup> and 1,271\u2009cm<jats:sup>\u22121<\/jats:sup>, and at 1,000\u2009cm<jats:sup>\u22121<\/jats:sup> and 1,450\u2009cm<jats:sup>\u22121<\/jats:sup>). Despite the small spectral differences between malignant lesions and benign tissues, the algorithms discriminated the spectra of non\u2010melanoma skin and pre\u2010cancerous lesions from benign and normal tissues, with an overall accuracy of 82.8% and 91.9%, respectively.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>PCA and PLS could discriminate Raman spectra of skin tissues, opening the way for an <jats:italic>in vivo<\/jats:italic> optical diagnosis. Lasers Surg. Med. 47:6\u201316, 2015. \u00a9 2015 Wiley Periodicals, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/lsm.22318","type":"journal-article","created":{"date-parts":[[2015,1,13]],"date-time":"2015-01-13T01:20:23Z","timestamp":1421112023000},"page":"6-16","source":"Crossref","is-referenced-by-count":40,"title":["Discrimination of non\u2010melanoma skin lesions from non\u2010tumor human skin tissues <i>in vivo<\/i> using Raman spectroscopy and multivariate statistics"],"prefix":"10.1002","volume":"47","author":[{"given":"Fabricio L.","family":"Silveira","sequence":"first","affiliation":[{"name":"Biomedical Engineering Institute Universidade Camilo Castelo Branco\u2010UNICASTELO Parque Tecnol\u00f3gico de S\u00e3o Jos\u00e9 dos Campos Estrada Dr. Altino Bondesan, 500, Eug\u00eanio de Melo S\u00e3o Jos\u00e9 dos Campos S\u00e3o Paulo Brazil 122447\u2010016"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcos T.T.","family":"Pacheco","sequence":"additional","affiliation":[{"name":"Biomedical Engineering Institute Universidade Camilo Castelo Branco\u2010UNICASTELO Parque Tecnol\u00f3gico de S\u00e3o Jos\u00e9 dos Campos Estrada Dr. Altino Bondesan, 500, Eug\u00eanio de Melo S\u00e3o Jos\u00e9 dos Campos S\u00e3o Paulo Brazil 122447\u2010016"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benito","family":"Bodanese","sequence":"additional","affiliation":[{"name":"Health Sciences Center\u2010CCS Universidade Comunit\u00e1ria Regional de Chapec\u00f3 \u2010 UNOCHAPEC\u00d3 Av. Sen. Att\u00edlio Fontana, 591\u2010E Chapec\u00f3 Santa Catarina Brazil 89809\u2010000"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlos A.","family":"Pasqualucci","sequence":"additional","affiliation":[{"name":"Department of Cardiovascular Pathology, Faculty of Medicine Universidade de S\u00e3o Paulo \u2010 USP Av. 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