{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,11]],"date-time":"2026-07-11T04:25:37Z","timestamp":1783743937493,"version":"3.55.0"},"reference-count":28,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2018,5,1]],"date-time":"2018-05-01T00:00:00Z","timestamp":1525132800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Portuguese Science Foundation","award":["007630UID\/QUI\/00313\/2013"],"award-info":[{"award-number":["007630UID\/QUI\/00313\/2013"]}]},{"name":"Portuguese Science Foundation","award":["PTDC\/QEQ6MED\/3521\/2014"],"award-info":[{"award-number":["PTDC\/QEQ6MED\/3521\/2014"]}]},{"name":"Portuguese Science Foundation","award":["Roteiro\/0152\/2013\/022124"],"award-info":[{"award-number":["Roteiro\/0152\/2013\/022124"]}]},{"name":"National Science Centre - Poland","award":["2013\/11\/D\/ST5\/02995"],"award-info":[{"award-number":["2013\/11\/D\/ST5\/02995"]}]},{"name":"National Science Centre - Poland","award":["2016\/22\/E\/NZ7\/00420"],"award-info":[{"award-number":["2016\/22\/E\/NZ7\/00420"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Lasers Surg Med"],"published-print":{"date-parts":[[2018,7]]},"abstract":"<jats:sec><jats:title>Objective<\/jats:title><jats:p>To compare hydrophilic and lipophilic bacteriochlorin photosensitizers in the photodynamic therapy of cancer, and relate their properties and <jats:italic>in vitro<\/jats:italic> phototoxicities to the efficacy of <jats:italic>in vivo<\/jats:italic> PDT treatments.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>Photochemical characterization of a hydrophilic bacteriochlorin (F<jats:sub>2<\/jats:sub>BOH) photosensitizer, and its use in PDT was compared with the performance of a closely related but water\u2010insoluble bacteriochlorin (F<jats:sub>2<\/jats:sub>BMet or redaporfin). Biodistribution, pharmacokinetics, skin photosensitivity, PDT efficacy and immune responses of two bacteriochlorins were compared. PDT <jats:italic>in vitro<\/jats:italic> employed CT26 colon carcinoma cells. BALB\/c mice bearing CT26 cells were treated according to a protocol where the illumination of the subcutaneous tumor is performed 15\u2009minute after intravenous administration of the photosensitizer, while it is in the vascular compartment (vascular\u2010PDT).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>F<jats:sub>2<\/jats:sub>BOH has photochemical properties comparable to redaporfin and both are promising photosensitizers for PDT. Although, F<jats:sub>2<\/jats:sub>BOH is 10 times less phototoxic <jats:italic>in vitro<\/jats:italic> than redaporfin, the phototoxicity of F<jats:sub>2<\/jats:sub>BOH in vascular\u2010PDT is comparable to that of redaporfin. This is consistent with the fact that the vasculature is the main target of vascular\u2010PDT. F<jats:sub>2<\/jats:sub>BOH\u2010PDT led to long\u2010term cures and stimulation of the immune system.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>F<jats:sub>2<\/jats:sub>BOH is soluble in aqueous media, photostable, has a convenient elimination half\u2010life of 44\u2009hours and leads to very low skin photosensitivity one week after administration. F<jats:sub>2<\/jats:sub>BOH and redaporfin are both very phototoxic in vascular\u2010PDT, but this could not be anticipated from their widely different phototherapeutic indices <jats:italic>in vitro<\/jats:italic>. PDT with F<jats:sub>2<\/jats:sub>BOH enabled long\u2010term cures of BALB\/c mice with subcutaneously implanted CT26 tumors, and the cured mice rejected tumor re\u2010inoculation one year after the treatment. Lasers Surg. Med. 50:451\u2013459, 2018. \u00a9 2018 Wiley Periodicals, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/lsm.22931","type":"journal-article","created":{"date-parts":[[2018,5,1]],"date-time":"2018-05-01T09:06:39Z","timestamp":1525165599000},"page":"451-459","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":29,"title":["Translating phototherapeutic indices from in vitro to in vivo photodynamic therapy with bacteriochlorins"],"prefix":"10.1002","volume":"50","author":[{"given":"Andr\u00e9 F.S.","family":"Luz","sequence":"first","affiliation":[{"name":"Chemistry Department University of Coimbra 3004\u2013535 Coimbra Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barbara","family":"Pucelik","sequence":"additional","affiliation":[{"name":"Faculty of Chemistry Jagiellonian University Gronostajowa 2 30\u20133867 Krakow Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariette M.","family":"Pereira","sequence":"additional","affiliation":[{"name":"Chemistry Department University of Coimbra 3004\u2013535 Coimbra Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Janusz M.","family":"D\u0105browski","sequence":"additional","affiliation":[{"name":"Faculty of Chemistry Jagiellonian University Gronostajowa 2 30\u20133867 Krakow Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luis G.","family":"Arnaut","sequence":"additional","affiliation":[{"name":"Chemistry Department University of Coimbra 3004\u2013535 Coimbra Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,5]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1040-8428(84)80016-5"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/90.12.889"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.20114"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1039\/C5PP00132C"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1572-1000(04)00007-9"},{"key":"e_1_2_7_7_1","first-page":"110","volume-title":"CRC handbook of organic photochemistry and photobiology","author":"Jori G","year":"2004"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-385904-4.00006-8"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jphotobiol.2010.06.004"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/chem.201304202"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tet.2010.09.106"},{"key":"e_1_2_7_12_1","unstructured":"Gomes\u2010da\u2010SilvaLC ZhaoL ZhouH et al. 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