{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,27]],"date-time":"2025-11-27T10:44:06Z","timestamp":1764240246571,"version":"3.37.3"},"reference-count":42,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2019,7,6]],"date-time":"2019-07-06T00:00:00Z","timestamp":1562371200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Lasers Surg Med"],"published-print":{"date-parts":[[2019,12]]},"abstract":"<jats:sec><jats:title>Background and Objectives<\/jats:title><jats:p>Wound contracture formation from excessive myofibroblast activity can result in debilitating morbidities. There are currently no treatments to prevent contracture. Photochemical tissue passivation (PTP), an established, safe, and user\u2010friendly treatment modality, crosslinks collagen by a light\u2010activated process, thus modulating the wound healing response and scarring. We hypothesised that PTP treatment would reinforce wounds by blunting the fibrotic response thus limiting contracture.<\/jats:p><\/jats:sec><jats:sec><jats:title>Study Design\/Materials and Methods<\/jats:title><jats:p>Full\u2010thickness, 1\u2009cm\u2009\u00d7\u20091\u2009cm excisional wounds were created on the dorsum of 32 C57BL\/6 mice. Treated wounds were painted with photosensitizing dye and exposed to visible light. Wounds were serially photographed over 6 weeks to measure wound contracture. At 7, 14, 21, and 42 days after wound creation, mice were euthanized and wounds were harvested for histologic review by a dermatopathologist.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>By Day 7, control wounds had significantly more contracture than those treated with PTP (33.0\u2009\u00b1\u200917.1% and 19.3\u2009\u00b1\u20099.0%, respectively; <jats:italic>P<\/jats:italic>\u2009=\u20090.011). PTP\u2010treated wounds maintained approximately 20% less contracture than controls from Day 14 and on (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05). By Day 42, wounds had contracted by 86.9\u2009\u00b1\u20095.5% in controls and 64.2\u2009\u00b1\u20093.2% in PTP\u2010treated wounds (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.03). Histologically, PTP wounds had earlier growth and development of dermal collagen, neovascularization, and development of skin appendages, compared with control wounds.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>PTP significantly limits contracture of full\u2010thickness wounds and improves wound healing. PTP\u2010treated wounds histologically demonstrate more mature structural organization than untreated wounds and closely resemble native skin. PTP treatment may be applicable not only for excisional wounds, but also for wounds with a high incidence of contracture and associated morbidity. Lasers Surg. Med. \u00a9 2019 Wiley Periodicals, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/lsm.23128","type":"journal-article","created":{"date-parts":[[2019,7,6]],"date-time":"2019-07-06T11:53:34Z","timestamp":1562414014000},"page":"910-919","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Photochemical Tissue Passivation Prevents Contracture of Full Thickness Wounds in Mice"],"prefix":"10.1002","volume":"51","author":[{"given":"Rachel L.","family":"Goldstein","sequence":"first","affiliation":[{"name":"Division of Plastic and Recontructive Surgery, Department of Surgery, Harvard Medical School Massachusetts General Hospital  55 Fruit Street Boston Massachusetts 02114"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6170-0201","authenticated-orcid":false,"given":"Jane M.","family":"Tsui","sequence":"additional","affiliation":[{"name":"Division of Plastic and Recontructive Surgery, Department of Surgery, Harvard Medical School Massachusetts General Hospital  55 Fruit Street Boston Massachusetts 02114"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gem","family":"Runyan","sequence":"additional","affiliation":[{"name":"Division of Plastic and Recontructive Surgery, Department of Surgery, Harvard Medical School Massachusetts General Hospital  55 Fruit Street Boston Massachusetts 02114"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mark A.","family":"Randolph","sequence":"additional","affiliation":[{"name":"Division of Plastic and Recontructive Surgery, Department of Surgery, Harvard Medical School Massachusetts General Hospital  55 Fruit Street Boston Massachusetts 02114"},{"name":"Wellman Center for Photomedicine, Harvard Medical School Massachusetts General Hospital  55 Fruit Street Boston Massachusetts 02114"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael C.","family":"McCormack","sequence":"additional","affiliation":[{"name":"Division of Plastic and Recontructive Surgery, Department of Surgery, Harvard Medical School Massachusetts General Hospital  55 Fruit Street Boston Massachusetts 02114"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"suffix":"Jr.","given":"Martin C.","family":"Mihm","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Harvard Medical School Brigham and Women's Hospital  75 Francis St Boston Massachusetts 02115"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert W.","family":"Redmond","sequence":"additional","affiliation":[{"name":"Wellman Center for Photomedicine, Harvard Medical School Massachusetts General Hospital  55 Fruit Street Boston Massachusetts 02114"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"suffix":"Jr.","given":"William G.","family":"Austen","sequence":"additional","affiliation":[{"name":"Division of Plastic and Recontructive Surgery, Department of Surgery, Harvard Medical School Massachusetts General Hospital  55 Fruit Street Boston Massachusetts 02114"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,7,6]]},"reference":[{"key":"e_1_2_7_1_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm809"},{"key":"e_1_2_7_1_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.burns.2014.02.011"},{"key":"e_1_2_7_1_4_1","first-page":"9","article-title":"Prevention and treatment of excessive dermal scarring","volume":"96","author":"Roseborough I","year":"2004","journal-title":"J Natl Med Assoc"},{"key":"e_1_2_7_1_5_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.PRS.0000101530.33096.5B"},{"key":"e_1_2_7_1_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1524-4725.2008.34406.x"},{"key":"e_1_2_7_1_7_1","doi-asserted-by":"publisher","DOI":"10.12688\/f1000research.8190.1"},{"key":"e_1_2_7_1_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-0-387-68113-9_10"},{"key":"e_1_2_7_1_9_1","unstructured":"NEISS. All injury program operated by the consumer product safety commission for numbers of injuries.2007\u20102015."},{"key":"e_1_2_7_1_10_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.326.7380.88"},{"key":"e_1_2_7_1_11_1","doi-asserted-by":"publisher","DOI":"10.1097\/BCR.0000000000000380"},{"key":"e_1_2_7_1_12_1","doi-asserted-by":"publisher","DOI":"10.1097\/BCR.0000000000000341"},{"key":"e_1_2_7_1_13_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.BCR.0000245652.26648.36"},{"key":"e_1_2_7_1_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00403-006-0651-7"},{"key":"e_1_2_7_1_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bjps.2014.04.011"},{"key":"e_1_2_7_1_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.burns.2006.01.016"},{"key":"e_1_2_7_1_17_1","doi-asserted-by":"publisher","DOI":"10.4103\/0970-0358.70724"},{"key":"e_1_2_7_1_18_1","doi-asserted-by":"publisher","DOI":"10.1097\/PRS.0b013e3181c82dd5"},{"key":"e_1_2_7_1_19_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.prs.0000438063.31043.79"},{"key":"e_1_2_7_1_20_1","doi-asserted-by":"publisher","DOI":"10.1161\/JAHA.116.003856"},{"key":"e_1_2_7_1_21_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.2006.0153"},{"key":"e_1_2_7_1_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1524-475X.2006.00188.x"},{"key":"e_1_2_7_1_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jss.2011.07.007"},{"key":"e_1_2_7_1_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1524-475X.2012.00856.x"},{"key":"e_1_2_7_1_25_1","doi-asserted-by":"publisher","DOI":"10.1155\/2011\/969618"},{"key":"e_1_2_7_1_26_1","doi-asserted-by":"publisher","DOI":"10.1097\/00000658-199908000-00017"},{"key":"e_1_2_7_1_27_1","doi-asserted-by":"publisher","DOI":"10.1089\/pho.2004.22.281"},{"key":"e_1_2_7_1_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2014.12.003"},{"key":"e_1_2_7_1_29_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1524-475X.2011.00712.x"},{"key":"e_1_2_7_1_30_1","doi-asserted-by":"publisher","DOI":"10.1097\/PRS.0b013e3182a3c091"},{"key":"e_1_2_7_1_31_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.tea.2010.0125"},{"key":"e_1_2_7_1_32_1","doi-asserted-by":"publisher","DOI":"10.1097\/00006534-199803000-00028"},{"key":"e_1_2_7_1_33_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1115973108"},{"key":"e_1_2_7_1_34_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.2004.10.1054"},{"key":"e_1_2_7_1_35_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.tec.2009.0511"},{"key":"e_1_2_7_1_36_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.teb.2014.0290"},{"key":"e_1_2_7_1_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.archoralbio.2007.11.003"},{"key":"e_1_2_7_1_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2010.08.049"},{"key":"e_1_2_7_1_39_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aai8792"},{"key":"e_1_2_7_1_40_1","doi-asserted-by":"publisher","DOI":"10.1021\/bm4011062"},{"key":"e_1_2_7_1_41_1","doi-asserted-by":"publisher","DOI":"10.1111\/wrr.12267"},{"key":"e_1_2_7_1_42_1","doi-asserted-by":"publisher","DOI":"10.1097\/00004630-198801000-00018"},{"issue":"6","key":"e_1_2_7_1_43_1","first-page":"12","article-title":"PDGF and FGF stimulate wound healing in the genetically diabetic mouse","volume":"136","author":"Greenhalgh DG","year":"1990","journal-title":"Am J Pathol"}],"container-title":["Lasers in Surgery and Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/lsm.23128","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/lsm.23128","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/lsm.23128","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,9]],"date-time":"2023-09-09T05:38:10Z","timestamp":1694237890000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/lsm.23128"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,7,6]]},"references-count":42,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2019,12]]}},"alternative-id":["10.1002\/lsm.23128"],"URL":"https:\/\/doi.org\/10.1002\/lsm.23128","archive":["Portico"],"relation":{},"ISSN":["0196-8092","1096-9101"],"issn-type":[{"type":"print","value":"0196-8092"},{"type":"electronic","value":"1096-9101"}],"subject":[],"published":{"date-parts":[[2019,7,6]]},"assertion":[{"value":"2019-06-01","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-07-06","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}