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The cells were analyzed using mass spectrometry for proteomics\/phosphoproteomics, AlamarBlue assay for mitochondrial activity, time\u2010lapse video for cell migration, quantitative polymerase chain reaction for gene expression, and immunofluorescence for protein expression.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Proteomic\/phosphoproteomic analysis showed inhibition of extracellular signal\u2010regulated kinases\/mammalian target of rapamycin and casein kinase 2 pathways, cell motility\u2010related networks, and multiple metabolic processes, including carbon metabolism, biosynthesis of amino acid, glycolysis\/gluconeogenesis, and the pentose phosphate pathway. Functional analysis demonstrated inhibition of mitochondrial activities, cell migration, and mitosis. Expression of growth promoting insulin\u2010like growth factor 1 and fibrosis\u2010related genes, including transforming growth factor \u03b21 (TGF\u03b21) and collagen type 1 \u02512 chain diminished. Protein expression of \u03b1\u2010smooth muscle actin, an important regulator of myofibroblast functions, was also suppressed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Low\u2010level blue light exerted suppressive effects on AMDFs, including suppression of mitochondrial activity, metabolism, cell motility, proliferation, TGF\u03b21 levels, and collagen I production. Low\u2010level blue light can be a potential treatment for the prevention and reduction of tissue fibrosis, such as hypertrophic scar and keloids. Lasers Surg. 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