{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T15:19:25Z","timestamp":1777389565603,"version":"3.51.4"},"reference-count":33,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2019,7,25]],"date-time":"2019-07-25T00:00:00Z","timestamp":1564012800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Lasers Surg Med"],"published-print":{"date-parts":[[2020,3]]},"abstract":"<jats:sec><jats:title>Background and Objectives<\/jats:title><jats:p>Successful outcomes of clinical studies for acne vulgaris depend greatly on achieving statistically significant reduction in acne lesion count and improvement in Investigator's Global Assessment score of the investigational drug product against its vehicle control. To date, there has not been a validated preclinical acne model to evaluate investigational drug products in order to improve the probability of clinical success. An inflammatory acne\u2010like lesion mouse model developed in\u2010house has previously been used for clinical guidance in our drug development program. In this study, we aim to implement and assess the adequacy of swept\u2010source optical coherence tomography (SS\u2010OCT) in quantifying the dynamic changes in inflammatory acne\u2010like lesions.<\/jats:p><\/jats:sec><jats:sec><jats:title>Study Design\/Materials and Methods<\/jats:title><jats:p>Live <jats:italic>Propionibacterium acnes<\/jats:italic> bacteria were injected intradermally resulting in inflammatory acne\u2010like lesions. Topical 1% and 2% minocycline gels were applied to the lesions in separate groups once daily for 2 weeks and compared with vehicle and untreated control groups. The growth of these lesions was monitored and measured with a ruler (height)\/microcaliper (width)\u2014an approach previously developed, and with SS\u2010OCT. The reliability of the two methods were assessed. Acquired OCT images across the apex of these inflammatory lesions were statistically analyzed for lesion volume reduction from baseline as well as between the treatment groups and the control groups.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The OCT technique allowed for reliable lesion volume analysis with varying conic profiles. After 14 days of topical minocycline treatments (1%, 2% minocycline), statistically significant reduction in lesion volume (<jats:italic>P<\/jats:italic>\u2009\u2264\u20090.05) based on OCT image analysis was observed compared with untreated and vehicle control groups as well as compared with baseline measurements. Under the right conditions, some morphological aspects of the \n<jats:italic>P. acnes<\/jats:italic> injection site were discernible within the skin in images captured with OCT.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>We demonstrated the first use of SS\u2010OCT in evaluating <jats:italic>in vivo<\/jats:italic> inflammatory acne\u2010like lesions in a murine model. Our findings support the use of OCT in assessing lesion size and evolution of <jats:italic>P. acnes<\/jats:italic> injection sites non\u2010invasively in preclinical <jats:italic>in vivo<\/jats:italic> studies, which could potentially lead to more consistent and predictable outcomes in clinical development. Lasers Surg. Med. \u00a9 2019 Wiley Periodicals, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/lsm.23140","type":"journal-article","created":{"date-parts":[[2019,7,26]],"date-time":"2019-07-26T06:23:15Z","timestamp":1564122195000},"page":"207-217","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["First Use of Optical Coherence Tomography on <i>In Vivo<\/i> Inflammatory Acne\u2010Like Lesions: A Murine Model"],"prefix":"10.1002","volume":"52","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3355-5614","authenticated-orcid":false,"given":"Maiko","family":"Hermsmeier","sequence":"first","affiliation":[{"name":"Research and Development BioPharmX, Inc. 115 Nicholson Lane San Jose California 95134"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tanvee","family":"Sawant","sequence":"additional","affiliation":[{"name":"Research and Development BioPharmX, Inc. 115 Nicholson Lane San Jose California 95134"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Khadiza","family":"Chowdhury","sequence":"additional","affiliation":[{"name":"Research and Development BioPharmX, Inc. 115 Nicholson Lane San Jose California 95134"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Usha","family":"Nagavarapu","sequence":"additional","affiliation":[{"name":"Research and Development BioPharmX, Inc. 115 Nicholson Lane San Jose California 95134"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0085-8210","authenticated-orcid":false,"given":"Kin F.","family":"Chan","sequence":"additional","affiliation":[{"name":"Research and Development BioPharmX, Inc. 115 Nicholson Lane San Jose California 95134"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,7,25]]},"reference":[{"key":"e_1_2_7_1_2_1","unstructured":"Acne. American Academy of Dermatology website.https:\/\/www.aad.org\/public\/diseases\/acne\u2010and\u2010rosacea\/acne#overview. Accessed September 17 2018."},{"key":"e_1_2_7_1_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0190-9622(98)70442-6"},{"key":"e_1_2_7_1_4_1","unstructured":"U.S. Department of Health and Human Services Food and Drug Administration (FDA) Center for Drug Evaluation and Research (CDER). Guidance for Industry: Acne Vulgaris: Developing Drugs for Treatment\u2014Draft guidance. J:\/!GUIDANC\/6499dftcln3.doc. Accessed September 18 2018."},{"key":"e_1_2_7_1_5_1","doi-asserted-by":"publisher","DOI":"10.4103\/0378-6323.51258"},{"key":"e_1_2_7_1_6_1","unstructured":"U.S. Department of Health and Human Services Food and Drug Administration (FDA) Center for Drug Evaluation and Research (CDER). Guidance for Industry: Acne Vulgaris: Establishing Effectiveness of Drugs Intended for Treatment.https:\/\/www.fda.gov\/downloads\/Drugs\/Guidances\/UCM071292.pdf. Accessed September 18 2018."},{"key":"e_1_2_7_1_7_1","unstructured":"HermsmeierM SawantT LacD et al. Evaluation of a topical minocycline gel in an inflammatory acne\u2010like lesion murine model. 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