{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,11]],"date-time":"2026-04-11T12:23:34Z","timestamp":1775910214077,"version":"3.50.1"},"reference-count":35,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2020,12,1]],"date-time":"2020-12-01T00:00:00Z","timestamp":1606780800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Lasers Surg Med"],"published-print":{"date-parts":[[2021,8]]},"abstract":"<jats:sec><jats:title>Background and Objectives<\/jats:title><jats:p>Light\u2010sheet microscopy (LSM) is a novel imaging technology that has been used for imaging fluorescence contrast in basic life science research. In this paper, we have developed a scattering\u2010based LSM (sLSM) for rapidly imaging the cellular morphology of fresh tissues without any exogenous fluorescent dyes.<\/jats:p><\/jats:sec><jats:sec><jats:title>Study Design\/Materials and Methods<\/jats:title><jats:p>In the sLSM device, a thin light sheet with the central wavelength of 834\u2009nm was incident on the tissue obliquely, 45\u00b0 relative to the tissue surface. The detection optics was configured to map the light sheet\u2010illuminated area onto a two\u2010dimensional imaging sensor. The illumination numerical aperture (NA) was set as 0.0625, and the detection NA 0.3.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The sLSM device achieved a light sheet thickness of less than 6.7\u2009\u00b5m over 284\u2009\u00b5m along the illumination optical axis. The detection optics of the sLSM device had a resolution of 1.8\u2009\u00b5m. The sLSM images of the swine kidney <jats:italic>ex vivo<\/jats:italic> visualized tubules with similar sizes and shapes to those observed in histopathologic images. The swine duodenum sLSM images revealed cell nuclei and villi architecture in superficial lesions and glands in deeper regions.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The preliminary results suggest that sLSM may have the potential for rapidly examining the freshly\u2010excised tissue <jats:italic>ex vivo<\/jats:italic> or intact tissue <jats:italic>in vivo<\/jats:italic> at microscopic resolution. Further optimization and performance evaluation of the sLSM technology will be needed in the future. Lasers Surg. Med. \u00a9 2020 Wiley Periodicals LLC<\/jats:p><\/jats:sec>","DOI":"10.1002\/lsm.23361","type":"journal-article","created":{"date-parts":[[2020,12,1]],"date-time":"2020-12-01T23:48:35Z","timestamp":1606866515000},"page":"872-879","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Scattering\u2010Based Light\u2010Sheet Microscopy for Rapid Cellular Imaging of Fresh Tissue"],"prefix":"10.1002","volume":"53","author":[{"given":"Christopher D.","family":"Nguyen","sequence":"first","affiliation":[{"name":"College of Optical Sciences University of Arizona  Tucson Arizona 85721"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patrick K.","family":"O'Neal","sequence":"additional","affiliation":[{"name":"College of Optical Sciences University of Arizona  Tucson Arizona 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