{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,30]],"date-time":"2026-10-30T18:29:27Z","timestamp":1793384967899,"version":"4.5.0"},"reference-count":76,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2021,4,13]],"date-time":"2021-04-13T00:00:00Z","timestamp":1618272000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["308067\/2014\u20102"],"award-info":[{"award-number":["308067\/2014\u20102"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["304607\/2018\u20105"],"award-info":[{"award-number":["304607\/2018\u20105"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Lasers Surg Med"],"published-print":{"date-parts":[[2021,11]]},"abstract":"<jats:p>\n<jats:bold>Background and Objectives<\/jats:bold>: Induction of myocardial infarction (MI) in rats by occlusion of the left anterior descending coronary artery is an experimental model used in research to elucidate functional, structural, and molecular modifications associated with ischemic heart disease. Photobiomodulation therapy (PBMT) has become a therapeutic alternative by modulating various biological processes eliciting several effects, including anti\u2010inflammatory and pro\u2010proliferative actions. The main objective of this work was to evaluate the effect of PBMT in the modulation of transcriptional and post\u2010transcriptional changes that occurred in myocardium signal transduction pathways after MI. <jats:bold>Study Design\/Materials and Methods<\/jats:bold>: Continuous wave (CW) non\u2010thermal laser parameters were: 660 nm wavelength, power 15 mW, with a total energy of 0.9 J, fluence of 1.15 J\/cm<jats:sup>2<\/jats:sup>, spot size of 0.785 cm<jats:sup>2<\/jats:sup>, and time of 60 seconds. Using <jats:italic>in silico<\/jats:italic> analysis, we selected and then, quantified the expression of messenger RNA (mRNA) of 47 genes of 9 signaling pathways associated with MI (angiogenesis, cell survival, hypertrophy, oxidative stress, apoptosis, extracellular matrix, calcium kinetics, cell metabolism, and inflammation). Messenger RNA expression quantification was performed in myocardial samples by polymerase chain reaction real\u2010time array using TaqMan customized plates. <jats:bold>Results<\/jats:bold>: Our results evidenced that MI modified mRNA expression of several well\u2010known biomarkers related to detrimental cardiac activity in almost all signaling pathways analyzed. However, PBMT reverted most of these transcriptional changes. More expressively, PBMT provoked a robust decrease in mRNA expression of molecules that participate in post\u2010MI inflammation and ECM composition, such as <jats:italic>IL\u20106, TNF receptor, TGFb1<\/jats:italic>, and collagen I and III. Global microRNA (miRNA) expression analysis revealed that PBMT decreased miR\u2010221, miR\u201034c, and miR\u201093 expressions post\u2010MI, which are related to deleterious effects in cardiac remodeling.<\/jats:p><jats:p>\n<jats:bold>Conclusion<\/jats:bold>: Thus, the identification of transcriptional and post\u2010transcriptional changes induced by PBMT may be used to interfere in the molecular dynamics of cardiac remodeling post\u2010MI.<\/jats:p>","DOI":"10.1002\/lsm.23407","type":"journal-article","created":{"date-parts":[[2021,4,13]],"date-time":"2021-04-13T07:16:24Z","timestamp":1618298184000},"page":"1247-1257","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Photobiomodulation Therapy on Myocardial Infarction in Rats: Transcriptional and Posttranscriptional Implications to Cardiac Remodeling"],"prefix":"10.1002","volume":"53","author":[{"given":"Regiane dos Santos","family":"Feliciano","sequence":"first","affiliation":[{"name":"Universidade Nove de Julho  Rua Vergueiro 249, Liberdade S\u00e3o Paulo, SP 01504\u2010001 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Allan Lu\u00eds Barboza","family":"Atum","sequence":"additional","affiliation":[{"name":"Universidade Nove de Julho  Rua Vergueiro 249, Liberdade S\u00e3o Paulo, SP 01504\u2010001 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"\u00c9rico Gustavo da Silva","family":"Ruiz","sequence":"additional","affiliation":[{"name":"Universidade Nove de Julho  Rua Vergueiro 249, Liberdade S\u00e3o Paulo, SP 01504\u2010001 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrey Jorge","family":"Serra","sequence":"additional","affiliation":[{"name":"Universidade Federal de S\u00e3o Paulo  Rua Pedro de Toledo 709, Vila Clementino S\u00e3o Paulo, SP 04039\u2010001 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ednei Luiz","family":"Ant\u00f4nio","sequence":"additional","affiliation":[{"name":"Universidade Federal de S\u00e3o Paulo  Rua Pedro de Toledo 709, Vila Clementino S\u00e3o Paulo, SP 04039\u2010001 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martha Trindade","family":"Manchini","sequence":"additional","affiliation":[{"name":"Universidade Nove de Julho  Rua Vergueiro 249, Liberdade S\u00e3o Paulo, SP 01504\u2010001 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jairo Montemor Augusto","family":"Silva","sequence":"additional","affiliation":[{"name":"Universidade Federal de S\u00e3o Paulo  Rua Pedro de Toledo 709, Vila Clementino S\u00e3o Paulo, SP 04039\u2010001 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paulo Jos\u00e9 Ferreira","family":"Tucci","sequence":"additional","affiliation":[{"name":"Universidade Federal de S\u00e3o Paulo  Rua Pedro de Toledo 709, Vila Clementino S\u00e3o Paulo, SP 04039\u2010001 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lubov","family":"Nathanson","sequence":"additional","affiliation":[{"name":"Nova Southeastern University  3301 College Avenue Fort Lauderdale Florida 33314"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariana","family":"Morris","sequence":"additional","affiliation":[{"name":"Nova Southeastern University  3301 College Avenue Fort Lauderdale Florida 33314"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Cristina","family":"Chavantes","sequence":"additional","affiliation":[{"name":"Universidade Nove de Julho  Rua Vergueiro 249, Liberdade S\u00e3o Paulo, SP 01504\u2010001 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 Ant\u00f4nio","family":"Silva J\u00fanior","sequence":"additional","affiliation":[{"name":"Universidade Nove de Julho  Rua Vergueiro 249, Liberdade S\u00e3o Paulo, SP 01504\u2010001 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,4,13]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1406656"},{"issue":"1","key":"e_1_2_7_3_1","first-page":"57","article-title":"Experimental myocardial infarction in the rat: Qualitative and quantitative changes during pathologic evolution","volume":"90","author":"Fishbein MC","year":"1978","journal-title":"Am J Pathol"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.44.4.503"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.2001.281.5.R1734"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.athoracsur.2005.05.103"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0008-6363(01)00434-5"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/cvr\/27.2.145"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1067\/mlc.2000.105971"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0008-6363(98)00139-4"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.1999.79.1.215"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2016.00565"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1177\/1074248415604463"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature09783"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCGENETICS.109.857425"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1196\/annals.1420.004"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0805038105"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0608791103"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1196\/annals.1302.029"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1093\/eurheartj\/ehl562"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/lsm.20377"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep42386"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0101270"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1590\/S0100-879X2006000500016"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijcard.2011.12.097"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cardfail.2008.10.017"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cardfail.2005.09.007"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0091017"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/bts188"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.0000130526.20854.fa"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.4070\/kcj.2009.39.10.393"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.01318.2005"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1002\/lsm.20896"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jphotobiol.2013.06.004"},{"issue":"3","key":"e_1_2_7_35_1","first-page":"387","article-title":"Phototherapy with low\u2010level laser influences the proliferation of endothelial cells and vascular endothelial growth factor and transforming growth factor\u2010beta secretion","volume":"64","author":"Szymanska J","year":"2013","journal-title":"J Physiol Pharmacol"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.2203\/dose-response.09-027.Hamblin"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10103-014-1646-9"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1002\/emmm.201202055"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.HYP.23.2.258"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tips.2008.05.003"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-0115(00)00105-1"},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.81.4.1161"},{"key":"e_1_2_7_43_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199108013250501"},{"key":"e_1_2_7_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/1043-6618(94)80100-2"},{"key":"e_1_2_7_45_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.89.5.2273"},{"key":"e_1_2_7_46_1","doi-asserted-by":"publisher","DOI":"10.1006\/phrs.2000.0758"},{"key":"e_1_2_7_47_1","doi-asserted-by":"publisher","DOI":"10.1006\/jmcc.2001.1467"},{"key":"e_1_2_7_48_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCHEARTFAILURE.108.812099"},{"key":"e_1_2_7_49_1","doi-asserted-by":"publisher","DOI":"10.1093\/eurheartj\/ehx177"},{"issue":"10","key":"e_1_2_7_50_1","first-page":"RA209","article-title":"Oxidative stress and ischemic myocardial syndromes","volume":"15","author":"Misra MK","year":"2009","journal-title":"Med Sci Monit"},{"key":"e_1_2_7_51_1","doi-asserted-by":"publisher","DOI":"10.1097\/00004872-200018060-00002"},{"key":"e_1_2_7_52_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0735-1097(02)01804-1"},{"issue":"4","key":"e_1_2_7_53_1","first-page":"315","article-title":"Metalloproteinases\u20102 and \u20109 predict left ventricular remodeling after myocardial infarction","volume":"100","author":"Cogni AL","year":"2013","journal-title":"Arq Bras Cardiol"},{"key":"e_1_2_7_54_1","doi-asserted-by":"publisher","DOI":"10.2147\/IJN.S129324"},{"key":"e_1_2_7_55_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejcts.2006.07.006"},{"key":"e_1_2_7_56_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cardiores.2005.10.002"},{"key":"e_1_2_7_57_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vascn.2010.02.011"},{"key":"e_1_2_7_58_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijcard.2010.04.053"},{"key":"e_1_2_7_59_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0008-6363(00)00003-1"},{"key":"e_1_2_7_60_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.112.4.435"},{"key":"e_1_2_7_61_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.5.4.439"},{"key":"e_1_2_7_62_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10103-018-2466-0"},{"key":"e_1_2_7_63_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10103-017-2410-8"},{"issue":"3","key":"e_1_2_7_64_1","first-page":"1291","article-title":"Modulation of tumor cell metabolism by laser photochemotherapy with cisplatin or zoledronic acid in vitro","volume":"38","author":"Heymann PGB","year":"2018","journal-title":"Anticancer Res"},{"key":"e_1_2_7_65_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10103-017-2176-z"},{"key":"e_1_2_7_66_1","doi-asserted-by":"publisher","DOI":"10.1155\/2013\/594906"},{"key":"e_1_2_7_67_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1011-1344(98)00219-X"},{"key":"e_1_2_7_68_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2017.00056"},{"key":"e_1_2_7_69_1","doi-asserted-by":"publisher","DOI":"10.1038\/cdd.2014.187"},{"key":"e_1_2_7_70_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.24075"},{"key":"e_1_2_7_71_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.heliyon.2018.e00639"},{"key":"e_1_2_7_72_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.08-128579"},{"key":"e_1_2_7_73_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrc2232"},{"key":"e_1_2_7_74_1","doi-asserted-by":"publisher","DOI":"10.2337\/diabetes.50.10.2363"},{"key":"e_1_2_7_75_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCEP.114.001973"},{"key":"e_1_2_7_76_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cellsig.2018.03.005"},{"key":"e_1_2_7_77_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2019.04.138"}],"container-title":["Lasers in Surgery and Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/lsm.23407","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/lsm.23407","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/lsm.23407","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,29]],"date-time":"2023-08-29T21:46:15Z","timestamp":1693345575000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/lsm.23407"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,4,13]]},"references-count":76,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2021,11]]}},"alternative-id":["10.1002\/lsm.23407"],"URL":"https:\/\/doi.org\/10.1002\/lsm.23407","archive":["Portico"],"relation":{},"ISSN":["0196-8092","1096-9101"],"issn-type":[{"value":"0196-8092","type":"print"},{"value":"1096-9101","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,4,13]]},"assertion":[{"value":"2020-10-23","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-03-22","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-04-13","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}