{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,19]],"date-time":"2026-02-19T01:02:27Z","timestamp":1771462947241,"version":"3.50.1"},"reference-count":15,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2021,7,23]],"date-time":"2021-07-23T00:00:00Z","timestamp":1626998400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100002642","name":"Korea University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002642","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Lasers Surg Med"],"published-print":{"date-parts":[[2022,2]]},"abstract":"Abstract<\/jats:title>Background and Objectives<\/jats:title>Lasers are known to be the most effective treatment modality for pigmentary skin diseases. However, melanocytes and melanin pigment often recur or leave post\u2010inflammatory hyperpigmentation after the laser procedure. Studies have reported on the role of progenitor cells in pigment cell regeneration, which can be constantly replenished through mitosis. However, the response of unpigmented melanocyte progenitor cells to laser treatment is poorly understood. In this study, we used adult zebrafish skin as the melanocyte regenerative system and examined the response of melanocyte progenitor cells to laser photothermolysis.<\/jats:p><\/jats:sec>Materials and Methods<\/jats:title>The two groups of adult zebrafish were irradiated with 1064\u2009nm wavelength laser system of Q\u2010switched neodymium:yttrium\u2013aluminum\u2013garnet (Nd:YAG) laser with 0.3 or 0.7\u2009J\u00b7cm\u22122<\/jats:sup>. We compared the regeneration of pigment at different energy levels by measuring new melanocyte counts and pigment area. We traced and quantitatively compared the melanocyte lineage cells by immunohistochemical staining using specific markers such as sox10, mitfa, and dct during the regeneration process. Three repetitive laser ablations were also held to test the postinflammatory hyperpigmentation.<\/jats:p><\/jats:sec>Results<\/jats:title>After the laser ablation of melanocytes, most of the new melanocytes appeared between Days 5 and 10. In high\u2010energy irradiation of 0.7\u2009J\u00b7cm\u22122<\/jats:sup>, the unpigmented mitfa\u2010expressing cells showed significant decrease (p<\/jats:italic>\u2009<\u20090.05) and showed delay in the differentiation process of melanocyte lineage cells. After repeated laser irradiation, hyperpigmentation did not appear and the final recovery ratio of the pigmented area was 87.5% and 75.3% at the 0.3 and 0.7\u2009J\u00b7cm\u22122<\/jats:sup> energy levels, respectively.<\/jats:p><\/jats:sec>Conclusion<\/jats:title>We suggest that laser treatment overcoming the recurrence should be planned based on the adequate energy level targeting the melanocyte progenitor cells. High\u2010energy irradiation may induce apoptosis of progenitor cells and delay their process of differentiation. Short\u2010term repetitive sessions of laser therapy can reduce the pigmentation in the long\u2010term observation.<\/jats:p><\/jats:sec>","DOI":"10.1002\/lsm.23458","type":"journal-article","created":{"date-parts":[[2021,7,23]],"date-time":"2021-07-23T21:37:12Z","timestamp":1627076232000},"page":"281-288","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Identification of the fate and regenerative mechanism of zebrafish melanocyte progenitor cells and melanocytes after laser\u2010induced pigment ablation"],"prefix":"10.1002","volume":"54","author":[{"given":"Ji Hyun","family":"Park","sequence":"first","affiliation":[{"name":"Department of Dermatology, College of Medicine Korea University Seoul Republic of Korea"},{"name":"Doctors Dermatologic Clinic Seoul Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Soonil","family":"Koun","sequence":"additional","affiliation":[{"name":"Incheon Technopark Bioindustry Center Incheon Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4141-204X","authenticated-orcid":false,"given":"Ko Eun","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Dermatology, College of Medicine Korea University Seoul Republic of Korea"},{"name":"Department of Dermatology Korea University Ansan Hospital Ansan\u2010si Gyeonggi\u2010do Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Il\u2010Hwan","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Dermatology, College of Medicine Korea University Seoul Republic of Korea"},{"name":"Department of Dermatology Korea University Ansan Hospital Ansan\u2010si Gyeonggi\u2010do Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,7,23]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.90.5.1790"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1242\/dmm.010371"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0749.1992.tb00562.x"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2015.04.025"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/jmicro\/dfq068"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.3109\/14764172.2012.738908"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/jid.2010.129"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.127.17.3715"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.126.17.3757"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1002265"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2016.06.020"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.00461"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0749.2004.00154.x"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.127.3.515"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/21.11.2703"}],"container-title":["Lasers in Surgery and Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/lsm.23458","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/lsm.23458","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/lsm.23458","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,25]],"date-time":"2023-08-25T19:46:55Z","timestamp":1692992815000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/lsm.23458"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,7,23]]},"references-count":15,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2022,2]]}},"alternative-id":["10.1002\/lsm.23458"],"URL":"https:\/\/doi.org\/10.1002\/lsm.23458","archive":["Portico"],"relation":{},"ISSN":["0196-8092","1096-9101"],"issn-type":[{"value":"0196-8092","type":"print"},{"value":"1096-9101","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,7,23]]},"assertion":[{"value":"2021-01-14","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-07-11","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-07-23","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}