{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T18:59:47Z","timestamp":1772132387063,"version":"3.50.1"},"reference-count":50,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2022,3,1]],"date-time":"2022-03-01T00:00:00Z","timestamp":1646092800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Lasers Surg Med"],"published-print":{"date-parts":[[2022,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background and Objectives<\/jats:title><jats:p>Single\u2010use of artesunate (ART) or 595\u2010nm pulsed\u2010dye laser (PDL) has proven\u00a0clinical efficacy in the treatment of hypertrophic scars (HSs), yet little research has been done on the combined use of ART and PDL. Bone morphogenetic protein\u20107 (BMP\u20107) and Fas are recognized to be two important proteins in reducing scar formation. This study was designed to observe the effect of ART combined with 595\u2010nm PDL in the treatment of HS in rabbit models, and investigate the effect of such protocol on the expression of BMP\u20107 and Fas in rabbit models.<\/jats:p><\/jats:sec><jats:sec><jats:title>Study Design\/Materials and Methods<\/jats:title><jats:p>Twenty\u2010four New Zealand white rabbits were randomly divided into the control group, ART group, PDL group, and combined treatment (ART\u2009+\u2009PDL) group. ART was respectively applied to the ART group and combined treatment group. Treatment was once every 2\u2010week for a total of three sessions for both groups. Animals in the PDL group were simply treated with 595\u2010nm PDL. Then, hematoxylin &amp; eosin and Van Gieson straining, immunohistochemical study, enzyme\u2010linked immunosorbent assay (ELISA), Cell counting kit\u20108 test, western blot assay, and real\u2010time polymerase chain reaction (RT\u2010PCR) were carried out to observe the development of HS samples and expression of BMP\u20107 and Fas proteins in the sample tissues.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>After treatment, the scar samples grew lower and flatter, which was particularly evident in the combined treatment group, with notably inhibited fibroblast and collagen compared to other groups (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.001). Western blot assay and RT\u2010PCR demonstrated that the expression of BMP\u20107 was most increased in scar samples treated by ART\u2009+\u2009PDL. BMP\u20107 level was correspondingly and notably upregulated in treatment groups, especially in the ART\u2009+\u2009PDL group. In addition, relevant expression of Fas was also higher after treatment, especially in the ART\u2009+\u2009PDL group compared to either ART or 595\u2010nm PDL group. The difference was significant among groups (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.001).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Combined use of ART and 595\u2010nm PDL can inhibit HSs in rabbit models via inhibiting extra fibroblast and collagens. The potential mechanism may be involved in enhanced BMP\u20107 and Fas expression. Our observations may create an alternative therapeutic strategy for HSs in the clinic.<\/jats:p><\/jats:sec>","DOI":"10.1002\/lsm.23518","type":"journal-article","created":{"date-parts":[[2022,3,2]],"date-time":"2022-03-02T07:37:55Z","timestamp":1646206675000},"page":"716-724","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Verifying the outcomes of artesunate plus 595\u2010nm PDL in hypertrophic scars via determining BMP\u20107 and Fas level in model rabbits"],"prefix":"10.1002","volume":"54","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8528-9602","authenticated-orcid":false,"given":"Jinxia","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Dermatology The Seventh Medical Center of PLA General Hospital Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wanting","family":"Luo","sequence":"additional","affiliation":[{"name":"Department of Dermatology The 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