{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T02:16:40Z","timestamp":1740104200781,"version":"3.37.3"},"reference-count":66,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2022,3,15]],"date-time":"2022-03-15T00:00:00Z","timestamp":1647302400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100005762","name":"National Taiwan University Hospital","doi-asserted-by":"publisher","award":["109S4567","110S5094"],"award-info":[{"award-number":["109S4567","110S5094"]}],"id":[{"id":"10.13039\/501100005762","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Lasers Surg Med"],"published-print":{"date-parts":[[2022,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objectives<\/jats:title><jats:p>Depilatory laser targeting melanin has been widely applied for the treatment of hypertrichosis. Both selective photothermolysis and thermal diffusion have been proposed for its effect, but the exact mechanism of permanent hair reduction remains unclear. In this study, we explore the role of thermal diffusion in depilatory laser\u2010induced permanent hair loss and determine whether nonpigmented cells are injured by thermal diffusion.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>C57BL\/6 mice in anagen and telogen were treated with alexandrite laser (wavelength 755\u2009nm, pulse duration 3 milliseconds, fluence 12\u2009J\/cm<jats:sup>2<\/jats:sup>, spot size 12\u2009mm), respectively. Histological analysis, terminal deoxynucleotidyl transferase dUTP nick\u2010end labeling\u00a0assay, and transmission electron microscopic imaging were employed to evaluate the injury to hair follicle (HF)\u00a0cells. The proliferation status of HF cells was examined by 5\u2010bromo\u20102\u2032\u2010deoxyuridine\u00a0pulse labeling. The number of HF stem cells was quantified by fluorescence\u2010activated cell sorting. The size of the regenerated hair was determined by measuring its length and width.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We found that irradiating C57BL\/6 mice in anagen with alexandrite laser led to hair miniaturization in the next anagen. In addition to thermal disruption of melanin\u2010containing cells in the precortex region, we also detected necrosis of the adjacent nonpigmented dermal papilla cells due to thermal diffusion. Dermal papilla cells decreased by 24% after laser injury, while the number of bulge stem cells remained unchanged. When the laser was delivered to telogen HFs where no melanin was present adjacent to the dermal papilla, thermal necrosis and cell reduction were not detected in the dermal papilla and no hair miniaturization was observed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Our results suggest that depilatory laser miniaturizes hair by inducing thermal necrosis of dermal papilla cells due to secondary thermal diffusion from melanin\u2010containing precortex cells in the anagen hair bulbs.<\/jats:p><\/jats:sec>","DOI":"10.1002\/lsm.23533","type":"journal-article","created":{"date-parts":[[2022,3,15]],"date-time":"2022-03-15T09:13:05Z","timestamp":1647335585000},"page":"916-927","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Depilatory laser miniaturizes hair by inducing bystander dermal papilla cell necrosis through thermal diffusion"],"prefix":"10.1002","volume":"54","author":[{"given":"Hsien\u2010Yi","family":"Chiu","sequence":"first","affiliation":[{"name":"Department of Biomedical Engineering, College of Medicine and College of Engineering National Taiwan University Taipei Taiwan"},{"name":"Department of Dermatology National Taiwan University Hospital Hsin\u2010Chu Branch Hsinchu Taiwan"},{"name":"Department of Dermatology National Taiwan University Hospital Taipei Taiwan"},{"name":"Department of Dermatology, College of Medicine National Taiwan University Taipei Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei\u2010Hung","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering, College of Medicine and College of Engineering National Taiwan University Taipei Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chen\u2010Hsiang","family":"Kuan","sequence":"additional","affiliation":[{"name":"Graduate Institute of Clinical Medicine, College of Medicine National Taiwan University Taipei Taiwan"},{"name":"Division of Plastic Surgery, Department of Surgery National Taiwan University Hospital and College of Medicine Taipei Taiwan"},{"name":"Research Center for Developmental Biology and Regenerative Medicine National Taiwan University Taipei Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1261-7273","authenticated-orcid":false,"given":"Yueh\u2010Feng","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering, College of Medicine and College of Engineering National Taiwan University Taipei Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chung\u2010Jen","family":"Tseng","sequence":"additional","affiliation":[{"name":"Department of Dermatology National Taiwan University Hospital Taipei Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wen\u2010Yen","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering, College of Medicine and College of Engineering National Taiwan University Taipei Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shiou\u2010Han","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Dermatology National Taiwan University Hospital Taipei Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1325-3464","authenticated-orcid":false,"given":"Sung\u2010Jan","family":"Lin","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering, College of Medicine and College of Engineering National Taiwan University Taipei Taiwan"},{"name":"Department of Dermatology National Taiwan University Hospital Taipei Taiwan"},{"name":"Department of Dermatology, College of Medicine National 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