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By combining relatively low\u2010intensity ultrasound and nanosecond\u2010pulse\u2010duration laser, we developed a novel treatment method, namely photo\u2010mediated ultrasound therapy (PUT), which holds a potential to remove RNV with minimal or no damage to the adjacent tissues.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>RNV was created in both albino and pigmented rabbits (<jats:italic>n<\/jats:italic>\u2009=\u200910) through a single intravitreal injection with DL\u2010\u03b1\u2010aminoadipic acid. RNV was treated with PUT 8 weeks postinjection. After PUT treatment, animals were evaluated longitudinally for up to 6 weeks. Treatment outcomes were evaluated through fundus photography, red\u2010free fundus photography, fluorescein angiography (FA), and histopathology.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>In both albino and pigmented rabbits, there were no leakage in the treatment area immediately after PUT treatment as demonstrated by FA, indicating the cessation of blood perfusion of the RNV in the treated area. The fluorescence leakage did not recover in albino rabbits during the 6\u2010week posttreatment monitoring period, and only 9.9\u2009\u00b1\u20099.8% of the neovascularization remained at the end of 6 weeks. In the pigmented rabbits, the fluorescence leakage partially returned, but the level of leakage decreased over time during the 6\u2010week posttreatment monitoring period, and only 10.8\u2009\u00b1\u20099.8% of the neovascularization remained at the end of 6 weeks. Histology demonstrated removal of vasculature without damage to the surrounding neurosensory retina.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>These results demonstrate that PUT could precisely remove RNV without damage to the surrounding neurosensory retina in both rabbit strains.<\/jats:p><\/jats:sec>","DOI":"10.1002\/lsm.23539","type":"journal-article","created":{"date-parts":[[2022,3,23]],"date-time":"2022-03-23T18:23:49Z","timestamp":1648059829000},"page":"747-757","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Photo\u2010mediated ultrasound therapy for the treatment of retinal neovascularization in rabbit eyes"],"prefix":"10.1002","volume":"54","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4196-9291","authenticated-orcid":false,"given":"Yu","family":"Qin","sequence":"first","affiliation":[{"name":"Department of Biomedical Engineering University of Michigan Ann Arbor Michigan USA"},{"name":"Institute of Acoustics, School of Physics Science and Engineering Tongji University Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yixin","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology and Visual Sciences University of Michigan Ann Arbor Michigan USA"},{"name":"Eye Center of Xiangya Hospital Central South University Changsha Hunan Province China"},{"name":"Hunan Key Laboratory of Ophthalmology Changsha Hunan Province China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Julia","family":"Fu","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology and Visual Sciences University of Michigan Ann Arbor Michigan USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4076-1643","authenticated-orcid":false,"given":"Mingyang","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering University of Michigan Ann Arbor Michigan USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xinmai","family":"Yang","sequence":"additional","affiliation":[{"name":"Institute for Bioengineering Research and Department of Mechanical Engineering University of Kansas Lawrence Kansas USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xueding","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering University of Michigan Ann Arbor Michigan USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0615-628X","authenticated-orcid":false,"given":"Yannis M.","family":"Paulus","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering University of Michigan Ann Arbor Michigan USA"},{"name":"Department of Ophthalmology and Visual Sciences University of Michigan Ann Arbor Michigan USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,3,23]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00109-013-0993-5"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmjopen-2018-022188"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12886-019-1292-x"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.2353\/ajpath.2007.061237"},{"key":"e_1_2_7_6_1","doi-asserted-by":"crossref","unstructured":"FossA RotsosT EmpeslidisT ChongV.The development of macular atrophy in patients with wet age\u2010related macular degeneration receiving anti\u2010VEGF treatment.Ophthalmologica. 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