{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T01:45:43Z","timestamp":1790732743888,"version":"4.1.0"},"reference-count":40,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2023,6,27]],"date-time":"2023-06-27T00:00:00Z","timestamp":1687824000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Lasers Surg Med"],"published-print":{"date-parts":[[2023,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Dynamic in vivo changes in melanin in melasma lesions after exposure to ultraviolet (UV) irradiation have not been described.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objectives<\/jats:title><jats:p>To determine whether melasma lesions and nearby perilesions demonstrated different adaptive responses to UV irradiation and whether the tanning responses were different among different locations on face.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We collected sequential images from real\u2010time cellular resolution full\u2010field optical coherence tomography (CRFF\u2010OCT) at melasma lesions and perilesions among 20 Asian patients. Quantitative and layer distribution analyses for melanin were performed using a computer\u2010aided detection (CADe) system that utilizes spatial compounding\u2010based denoising convolutional neural networks.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The detected melanin (D) is melanin with a diameter &gt;0.5\u2009\u00b5m, among which confetti melanin (C) has a diameter of &gt;3.3\u2009\u00b5m and corresponds to a melanosome\u2010rich package. The calculated C\/D ratio is proportional to active melanin transportation. Before UV exposure, melasma lesions had more detected melanin (<jats:italic>p<\/jats:italic>\u2009=\u20090.0271), confetti melanin (<jats:italic>p<\/jats:italic>\u2009=\u20090.0163), and increased C\/D ratio (<jats:italic>p<\/jats:italic>\u2009=\u20090.0152) in the basal layer compared to those of perilesions. After exposure to UV irradiation, perilesions have both increased confetti melanin (<jats:italic>p<\/jats:italic>\u2009=\u20090.0452) and the C\/D ratio (<jats:italic>p<\/jats:italic>\u2009=\u20090.0369) in basal layer, and this effect was most prominent in right cheek (<jats:italic>p<\/jats:italic>\u2009=\u20090.030). There were however no significant differences in the detected, confetti, or granular melanin areas before and after exposure to UV irradiation in melasma lesions in all the skin layers.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Hyperactive melanocytes with a higher baseline C\/D ratio were noted in the melasma lesions. They were \u201cfixed\u201d on the plateau and were not responsive to UV irradiation regardless of the location on face. Perilesions retained adaptability with a dynamic response to UV irradiation, in which more confetti melanin was shed, mainly in the basal layer. Therefore, aggravating effect of UV on melasma was mainly due to UV\u2010responsive perilesions rather than lesions.<\/jats:p><\/jats:sec>","DOI":"10.1002\/lsm.23699","type":"journal-article","created":{"date-parts":[[2023,6,27]],"date-time":"2023-06-27T07:58:46Z","timestamp":1687852726000},"page":"680-689","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Adaptability of melanocytes post ultraviolet stimulation in patients with melasma"],"prefix":"10.1002","volume":"55","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0425-9154","authenticated-orcid":false,"given":"Yen\u2010Jen","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Dermatology MacKay Memorial Hospital Taipei Taiwan"},{"name":"Department of Cosmetic Applications and Management MacKay Junior College of Medicine, Nursing, and Management New Taipei City Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0463-7346","authenticated-orcid":false,"given":"Chang\u2010Cheng","family":"Chang","sequence":"additional","affiliation":[{"name":"Department of Cosmeceutics China Medical University  Taichung Taiwan"},{"name":"Institute of Imaging and Biomedical Photonics National Yang Ming Chiao Tung University Tainan Taiwan"},{"name":"School of Medicine, College of Medicine China Medical University Hospital Taichung Taiwan"},{"name":"Aesthetic Medical Center China Medical University Hospital Taichung Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5579-2197","authenticated-orcid":false,"given":"Yu\u2010Hung","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Dermatology MacKay Memorial Hospital Taipei Taiwan"},{"name":"Department of Medicine Mackay Medical College New Taipei City Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8254-2548","authenticated-orcid":false,"given":"Ling","family":"Huang","sequence":"additional","affiliation":[{"name":"Clinical Development Apollo Medical Optics, Ltd. Taipei Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2568-4512","authenticated-orcid":false,"given":"Jia\u2010Wei","family":"Shen","sequence":"additional","affiliation":[{"name":"Department of Cosmeceutics China Medical University  Taichung Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2803-147X","authenticated-orcid":false,"given":"Meng\u2010En","family":"Lu","sequence":"additional","affiliation":[{"name":"Department of Cosmeceutics China Medical University  Taichung Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1586-910X","authenticated-orcid":false,"given":"Hsiu\u2010Mei","family":"Chiang","sequence":"additional","affiliation":[{"name":"Department of Cosmeceutics China Medical University  Taichung 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