{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T02:16:25Z","timestamp":1740104185768,"version":"3.37.3"},"reference-count":21,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2024,6,19]],"date-time":"2024-06-19T00:00:00Z","timestamp":1718755200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Lasers Surg Med"],"published-print":{"date-parts":[[2024,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Objectives<\/jats:title><jats:p>Fractional ablative CO<jats:sub>2<\/jats:sub> lasers are used clinically to treat cutaneous burn scars with reported varying degrees of effectiveness. It was hypothesized that different laser pulse energy settings may lead to differential gene transcription in a porcine model.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Uninjured skin from red Duroc pigs was treated with a fractional ablative CO<jats:sub>2<\/jats:sub> laser set to 70, 100, or 120\u2009mJ across the abdomen (<jats:italic>n<\/jats:italic>\u2009=\u20094 areas per treatment). Punch biopsies of both treated and untreated sites were taken before treatment (baseline), at 30\u2009min, and at each hour for 6\u2009h and stored in All\u2010Protect tissue reagent. The biopsies were then used to isolate RNA, which was subsequently used in qRT\u2010PCR for eight genes associated with wound healing and the extracellular matrix: CCL2, IL6, FGF2, TIMP1, TIMP3, COL1A2, MMP2, and\u00a0DCN. RPL13a was used as a housekeeping gene to normalize the eight\u00a0genes of interest. One\u2010way ANOVA tests were used to assess for differences among laser pulse energies and two\u2010way ANOVA tests were used to assess the differences between treated and untreated areas.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>While six of the eight genes were upregulated after treatment (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05), there were no significant differences in gene expression between the different laser pulse energies for any of the eight genes.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>While laser treatment is correlated with a positive and significant upregulation for six of the eight genes 4\u2009h after intervention, the pulse energy settings of the laser did not lead to a statistically significant difference in gene transcription among the treatment areas. Different laser pulse energies may not be required to induce similar cellular responses in a clinical setting.<\/jats:p><\/jats:sec>","DOI":"10.1002\/lsm.23813","type":"journal-article","created":{"date-parts":[[2024,6,20]],"date-time":"2024-06-20T06:49:41Z","timestamp":1718866181000},"page":"606-612","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Altering CO<sub>2<\/sub> Laser Pulse Energy Settings Did Not Influence Differential Gene Transcription in Normal Skin in a Red Duroc\u00a0Pig Model"],"prefix":"10.1002","volume":"56","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-4612-913X","authenticated-orcid":false,"given":"Lou'ay K.","family":"Hussein","sequence":"first","affiliation":[{"name":"Firefighters' Burn and Surgical Research Laboratory MedStar Health Research Institute Washington DC USA"},{"name":"West Virginia School of Osteopathic Medicine Lewisburg West Virginia USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Taryn E.","family":"Travis","sequence":"additional","affiliation":[{"name":"Firefighters' Burn and Surgical Research Laboratory MedStar Health Research Institute Washington DC USA"},{"name":"Department of Surgery Georgetown University School of Medicine Washington DC USA"},{"name":"Department of Surgery The Burn Center, MedStar Washington Hospital Center Washington DC USA"},{"name":"Department of Plastic and Reconstructive Surgery Georgetown University School of Medicine Washington DC USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lauren T.","family":"Moffatt","sequence":"additional","affiliation":[{"name":"Firefighters' Burn and Surgical Research Laboratory MedStar Health Research Institute Washington DC USA"},{"name":"Department of Surgery Georgetown University School of Medicine Washington DC USA"},{"name":"Department of Plastic and Reconstructive Surgery Georgetown University School of Medicine Washington DC USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jeffrey W.","family":"Shupp","sequence":"additional","affiliation":[{"name":"Firefighters' Burn and Surgical Research Laboratory MedStar Health Research Institute Washington DC USA"},{"name":"Department of Surgery Georgetown University School of Medicine Washington DC USA"},{"name":"Department of Surgery The Burn Center, MedStar Washington Hospital Center Washington DC USA"},{"name":"Department of Plastic and Reconstructive Surgery Georgetown University School of Medicine Washington DC USA"},{"name":"Department of Biochemistry and Molecular &amp; Cellular Biology Georgetown University School of Medicine Washington DC USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2292-4960","authenticated-orcid":false,"given":"Bonnie C.","family":"Carney","sequence":"additional","affiliation":[{"name":"Firefighters' Burn and Surgical Research Laboratory MedStar Health Research Institute Washington DC USA"},{"name":"Department of Surgery Georgetown University School of Medicine Washington DC USA"},{"name":"Department of Plastic and Reconstructive Surgery Georgetown University School of Medicine Washington DC USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,6,19]]},"reference":[{"key":"e_1_2_7_2_1","unstructured":"N.Verma S.Yumeen andB. 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