{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,10]],"date-time":"2025-12-10T03:55:26Z","timestamp":1765338926019,"version":"3.46.0"},"reference-count":39,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2025,9,24]],"date-time":"2025-09-24T00:00:00Z","timestamp":1758672000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Lasers Surg Med"],"published-print":{"date-parts":[[2025,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>To assess the efficacy and tolerability of Rose Bengal\u2010induced photochemical crosslinking (RB\u2010PCL) as an adjunct to suturing for corneal perforation repair in rabbits.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>A 4.5\u2009mm corneal wound was created in New Zealand White Rabbits (right eyes), followed by suturing and RB\u2010PCL treatment using 532\u2009nm green light (0.6\u2009W\/cm\u00b2) and 0.01% RB photosensitizer. A control group received sutures alone. Healing was evaluated at Weeks 1, 2, and 4 using slit\u2010lamp biomicroscopy, anterior segment optical coherence tomography (AS\u2010OCT), RT\u2010PCR for \u03b1\u2010SMA gene expression, and immunofluorescence for myofibroblast activation. Histopathological changes were assessed via Hematoxylin and Eosin (H&amp;E) staining, and corneal endothelial cell counts were taken to assess cytotoxicity.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>RB\u2010PCL accelerated suture removal by Day 5, while sutures in the control group required longer retention. At Weeks 1 and 2, RB\u2010PCL demonstrated lower inflammation and reduced corneal opacity. By Weeks 2 and 4, \u03b1\u2010SMA expression was significantly reduced in the RB\u2010PCL group, indicating reduced myofibroblast activation and fibrosis. H&amp;E staining showed complete healing in the RB\u2010PCL group by Week 2, whereas the control group had incomplete repair. By Week 4, the RB\u2010PCL group had better fiber arrangement, while the control group showed persistent fibrosis. Immunofluorescence confirmed reduced \u03b1\u2010SMA expression in the RB\u2010PCL group. No retinal damage was observed, and endothelial cell counts were similar between groups, indicating RB\u2010PCL's tolerability.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>RB\u2010PCL improved corneal wound healing, reduced fibrosis and opacity, and allowed earlier suture removal without phototoxicity. These results suggest RB\u2010PCL is a promising adjunct for corneal perforation repair, enhancing both efficacy and tolerability.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/lsm.70068","type":"journal-article","created":{"date-parts":[[2025,9,25]],"date-time":"2025-09-25T04:35:59Z","timestamp":1758774959000},"page":"809-820","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Photochemical Crosslinking as an Adjunct for Repairing Corneal Perforations in Rabbits"],"prefix":"10.1002","volume":"57","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-8252-8863","authenticated-orcid":false,"given":"Honghu","family":"Jiang","sequence":"first","affiliation":[{"name":"Department of Ophthalmology, Huadong Hospital Fudan University Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lu","family":"Zhu","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Huadong Hospital Fudan University Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lanting","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Huadong Hospital Fudan University Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hongyuan","family":"Ren","sequence":"additional","affiliation":[{"name":"Boston University Chobanian &amp; Avedisian School of Medicine Boston Massachusetts USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yan","family":"Yu","sequence":"additional","affiliation":[{"name":"RA Consulting Chalfont Pennsylvania USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jingyin","family":"Zhu","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Huadong Hospital Fudan University Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,9,24]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.4103\/ijo.IJO_1259_22"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jfo.2015.08.005"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/ceo.12511"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1080\/08164622.2024.2411415"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1177\/112067210801800114"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2007.08.041"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcrs.2006.10.039"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.1097\/00003226-200111000-00008"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.1001\/archopht.1968.00980050707004"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2019.01.011"},{"key":"e_1_2_12_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0142-9612(02)00002-9"},{"key":"e_1_2_12_13_1","doi-asserted-by":"publisher","DOI":"10.2147\/OPTH.S394454"},{"key":"e_1_2_12_14_1","doi-asserted-by":"publisher","DOI":"10.4103\/0301-4738.55079"},{"key":"e_1_2_12_15_1","doi-asserted-by":"publisher","DOI":"10.1089\/jop.1997.13.31"},{"key":"e_1_2_12_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.athoracsur.2004.11.054"},{"key":"e_1_2_12_17_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.joc.8b01545"},{"key":"e_1_2_12_18_1","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.19-26604"},{"key":"e_1_2_12_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2133.2011.10710.x"},{"key":"e_1_2_12_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/wrr.13202"},{"key":"e_1_2_12_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/lary.21797"},{"key":"e_1_2_12_22_1","doi-asserted-by":"publisher","DOI":"10.1155\/2020\/1753152"},{"key":"e_1_2_12_23_1","doi-asserted-by":"publisher","DOI":"10.1177\/1553350618824448"},{"key":"e_1_2_12_24_1","doi-asserted-by":"publisher","DOI":"10.3109\/08990220.2016.1142438"},{"key":"e_1_2_12_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/lsm.20548"},{"key":"e_1_2_12_26_1","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.03-1066"},{"key":"e_1_2_12_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcrs.2004.02.068"},{"key":"e_1_2_12_28_1","doi-asserted-by":"publisher","DOI":"10.1080\/02713683.2017.1344712"},{"key":"e_1_2_12_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcrs.2010.12.037"},{"key":"e_1_2_12_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep32352"},{"key":"e_1_2_12_31_1","first-page":"58","article-title":"Basement Membrane Regeneration and TGF\u2010\u03b21 Expression in Rabbits With Corneal Perforating Injury","volume":"29","author":"Meng N.","year":"2023","journal-title":"Molecular Vision"},{"key":"e_1_2_12_32_1","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.18-26451"},{"key":"e_1_2_12_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exer.2021.108803"},{"key":"e_1_2_12_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0039-6257(97)80029-X"},{"key":"e_1_2_12_35_1","doi-asserted-by":"publisher","DOI":"10.4142\/jvs.2004.5.3.267"},{"key":"e_1_2_12_36_1","doi-asserted-by":"publisher","DOI":"10.1097\/ICO.0000000000003476"},{"key":"e_1_2_12_37_1","doi-asserted-by":"publisher","DOI":"10.3109\/08820539409060012"},{"key":"e_1_2_12_38_1","first-page":"809","article-title":"Expression of Alpha\u2010Smooth Muscle (Alpha\u2010SM) Actin During Corneal Stromal Wound Healing","volume":"36","author":"Jester J. 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