{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,12]],"date-time":"2026-08-12T18:32:00Z","timestamp":1786559520999,"version":"build-2736575974"},"reference-count":21,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2026,5,18]],"date-time":"2026-05-18T00:00:00Z","timestamp":1779062400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,5,18]],"date-time":"2026-05-18T00:00:00Z","timestamp":1779062400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82003177"],"award-info":[{"award-number":["82003177"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Lasers Surg Med"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>\n                      This study aims to compare the therapeutic effects of hematoporphyrin monomethyl ether (HMME) and\n                      <jats:italic>meta<\/jats:italic>\n                      \u2010tetrahydroxyphenylchlorin (\n                      <jats:italic>m<\/jats:italic>\n                      \u2010THPC) under single\u2010wavelength and dual\u2010wavelength light irradiation in photodynamic therapy (PDT), and aims to optimize light source matching strategies to enhance targeted treatment of vascular lesions.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      This study employed the ABDA probe assay to evaluate the in vitro reactive oxygen species (ROS) generation efficiency of two photosensitizers (HMME and\n                      <jats:italic>m<\/jats:italic>\n                      \u2010THPC) under 410, 532, 653\u2009nm, and dual\u2010wavelength (410\u2009+\u2009653\u2009nm) irradiation. Using Leghorn chicken comb as a vascular malformation model, we further assessed in vivo vascular damage effects, thermal responses, tissue\u2010level ROS production, and apoptosis rates.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      This study demonstrated that HMME exhibited optimal photodynamic efficacy at 410\u2009nm, while\n                      <jats:italic>m<\/jats:italic>\n                      \u2010THPC maintained substantial photodynamic efficacy at both 410 and 653\u2009nm, with the dual\u2010wavelength combination (410\u2009+\u2009653\u2009nm) showing complementary activation for\n                      <jats:italic>m<\/jats:italic>\n                      \u2010THPC but reduced efficacy for HMME. The 532\u2009nm wavelength, though effective for vascular damage, caused significant thermal injury and non\u2010specific apoptosis of non\u2010target cells, highlighting its clinical limitations. Histopathological analysis confirmed that 410 and 653\u2009nm irradiation selectively targeted vasculature with minimal epidermal damage, whereas 532\u2009nm led to widespread tissue necrosis. ROS generation and apoptotic assays revealed that HMME's cytotoxicity was strongest at 410\u2009nm, while\n                      <jats:italic>m<\/jats:italic>\n                      \u2010THPC performed well across multiple wavelengths, and particularly benefited from dual\u2010wavelength activation.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      Photosensitizer activation efficiency is highly wavelength\u2010dependent, with HMME performing best at shorter wavelengths (410\u2009nm) while\n                      <jats:italic>m<\/jats:italic>\n                      \u2010THPC maintains considerable efficacy at longer wavelengths (653\u2009nm). The dual\u2010wavelength combination (410\u2009+\u2009653\u2009nm) can partially overcome limitations of single wavelengths, providing references for clinical light source optimization.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/lsm.70150","type":"journal-article","created":{"date-parts":[[2026,5,18]],"date-time":"2026-05-18T18:38:52Z","timestamp":1779129532000},"page":"550-563","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Light Source Matching Strategies for Optimal Treatment Efficacy of HMME and\n                    <i>m<\/i>\n                    \u2010THPC in Photodynamic Therapy of Vascular Lesions"],"prefix":"10.1002","volume":"58","author":[{"given":"Zhewen","family":"Song","sequence":"first","affiliation":[{"name":"Department of Oral and Maxillofacial\u2010Head Neck Oncology, Department of Laser and Aesthetic Medicine, Shanghai Ninth People's Hospital, College of Stomatology Shanghai Jiao Tong University School of Medicine Shanghai China"},{"name":"National Clinical Research Center for Oral Diseases Shanghai China"},{"name":"Shanghai Key Laboratory of Stomatology &amp; Shanghai Research Institute of Stomatology Shanghai China"},{"name":"Department of General Dentistry Pudong New District Ophthalmology and Odontology Dispensary Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuiting","family":"Fu","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial\u2010Head Neck Oncology, Department of Laser and Aesthetic Medicine, Shanghai Ninth People's Hospital, College of Stomatology Shanghai Jiao Tong University School of Medicine Shanghai China"},{"name":"National Clinical Research Center for Oral Diseases Shanghai China"},{"name":"Shanghai Key Laboratory of Stomatology &amp; Shanghai Research Institute of Stomatology Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Health 940 Hospital of PLA Joint Logistic Support Lanzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guoyu","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial\u2010Head Neck Oncology, Department of Laser and Aesthetic Medicine, Shanghai Ninth People's Hospital, College of Stomatology Shanghai Jiao Tong University School of Medicine Shanghai China"},{"name":"National Clinical Research Center for Oral Diseases Shanghai China"},{"name":"Shanghai Key Laboratory of Stomatology &amp; Shanghai Research Institute of Stomatology Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-7413-7212","authenticated-orcid":false,"given":"Ting","family":"Zhu","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial\u2010Head Neck Oncology, Department of Laser and Aesthetic Medicine, Shanghai Ninth People's Hospital, College of Stomatology Shanghai Jiao Tong University School of Medicine Shanghai China"},{"name":"National Clinical Research Center for Oral Diseases Shanghai China"},{"name":"Shanghai Key Laboratory of Stomatology &amp; Shanghai Research Institute of Stomatology Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,5,18]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaad.2025.02.037"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12094-008-0172-2"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1089\/pho.2023.0188"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.5946\/ce.2013.46.1.24"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.3390\/pharmaceutics13091332"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcis.2024.01.157"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1039\/d5bm00544b"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.3390\/biomedicines11010224"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pdpdt.2025.104564"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pdpdt.2020.101649"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pdpdt.2023.103837"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/jdv.16596"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pdpdt.2023.103933"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pdpdt.2023.103545"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms242316890"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms22136658"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.37349\/ebmx.2025.101337"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1039\/d3tb02423g"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00403-024-03531-x"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.34172\/jlms.2023.28"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms22052437"}],"container-title":["Lasers in Surgery and Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/lsm.70150","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/lsm.70150","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/lsm.70150","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,12]],"date-time":"2026-08-12T18:09:04Z","timestamp":1786558144000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/lsm.70150"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,5,18]]},"references-count":21,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2026,9]]}},"alternative-id":["10.1002\/lsm.70150"],"URL":"https:\/\/doi.org\/10.1002\/lsm.70150","archive":["Portico"],"relation":{},"ISSN":["0196-8092","1096-9101"],"issn-type":[{"value":"0196-8092","type":"print"},{"value":"1096-9101","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,5,18]]},"assertion":[{"value":"2026-02-14","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-05-11","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-05-18","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}