{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,3,29]],"date-time":"2025-03-29T16:56:47Z","timestamp":1743267407341,"version":"3.33.0"},"reference-count":51,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"4","license":[{"start":{"date-parts":[[2015,9,1]],"date-time":"2015-09-01T00:00:00Z","timestamp":1441065600000},"content-version":"tdm","delay-in-days":3440,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2006,4]]},"abstract":"<jats:sec>\n            <jats:title\/>\n            <jats:p>Transplantation of reduced-size livers may lead to a hypermetabolic state and increased production of oxygen radicals. Since oxygen radicals may cause liver injury and impair liver regeneration, we tested the hypothesis that overexpression of superoxide dismutase (SOD) in reduced-size livers (RSL) would accelerate regeneration and reduce injury in a rat model of transplantation of RSL. Donor rats were infected with adenoviruses either expressing SOD1 (Ad.SOD1) or beta-galactosidase (Ad.<jats:italic toggle=\"yes\">lacZ<\/jats:italic>). Livers were harvested 72 hours later, reduced to 45% of weight, and transplanted. After transplantation, hepatic SOD activity, graft survival, histopathology, AST\/ALT release, and bilirubin were examined. Regeneration was evaluated by BrdU-staining, graft weight, and expression of cyclin D1 and p21. In Ad.SOD1-treated livergrafts, SOD activity increased three-fold compared to controls. Survival was dramatically increased in recipients of Ad.SOD1-RSL (100% vs. 20% in Ad.<jats:italic toggle=\"yes\">lacZ<\/jats:italic>-RSL), and peak levels of AST\/ALT and bilirubin levels were reduced by 75% and 87.5%, respectively (<jats:italic toggle=\"yes\">P<\/jats:italic> &lt; 0.001). In histological sections, hepatocyte necrosis decreased from 24% after Ad.<jats:italic toggle=\"yes\">lacZ<\/jats:italic>\u2013treatment to 6% after Ad.SOD1-treatment (<jats:italic toggle=\"yes\">P<\/jats:italic> &lt;0.001). Regeneration was also accelerated after Ad.SOD1-treatment as demonstrated by an increase of BrdU-stained cells 24 hours after reperfusion and increased liver weight after 1 week. In conclusion, overexpression of SOD1 in RSL prevents primary non-function of reduced-size liver grafts and accelerates liver regeneration. <jats:bold>\n                                    Liver Transpl 12:550\u2013559, 2006.\n                                 <\/jats:bold>\n                                 <jats:bold>\u00a9 2006 AASLD.<\/jats:bold>\n                              <\/jats:p>\n          <\/jats:sec>","DOI":"10.1002\/lt.20632","type":"journal-article","created":{"date-parts":[[2006,3,23]],"date-time":"2006-03-23T00:17:14Z","timestamp":1143073034000},"page":"550-559","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":14,"title":["Minimizing oxidative stress by gene delivery of superoxide dismutase accelerates regeneration after transplantation of reduced-size livers in the rat"],"prefix":"10.1097","volume":"12","author":[{"given":"Thorsten G.","family":"Lehmann","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tom","family":"Luedde","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert F.","family":"Schwabe","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hartwig","family":"Bunzendahl","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jude R.","family":"Samulski","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John J.","family":"Lemasters","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David A.","family":"Brenner","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"276","reference":[{"key":"R1-10-20250124","doi-asserted-by":"crossref","first-page":"989","DOI":"10.1136\/bmj.312.7037.989","article-title":"Shortage of organs for transplantation editorial.","volume":"312","author":"Wight","year":"1996","journal-title":"Br Med J"},{"key":"R2-10-20250124","doi-asserted-by":"crossref","first-page":"519","DOI":"10.1097\/00007890-198803000-00003","article-title":"Liver transplantation with reducedsize donor organs.","volume":"45","author":"Broelsch","year":"1988","journal-title":"Transplantation"},{"key":"R3-10-20250124","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1097\/00000658-199007000-00003","article-title":"Transplantation of two patients with one liver. 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