{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,11]],"date-time":"2026-04-11T15:57:22Z","timestamp":1775923042763,"version":"3.50.1"},"reference-count":17,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"8","license":[{"start":{"date-parts":[[2009,8,1]],"date-time":"2009-08-01T00:00:00Z","timestamp":1249084800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"},{"start":{"date-parts":[[2009,7,29]],"date-time":"2009-07-29T00:00:00Z","timestamp":1248825600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Children's Hospital of Pittsburg Research Foundation","award":["5RO1AI073895-03"],"award-info":[{"award-number":["5RO1AI073895-03"]}]},{"name":"Children's Hospital of Pittsburg Research Foundation","award":["5RO1AI49156-05"],"award-info":[{"award-number":["5RO1AI49156-05"]}]},{"name":"Hillman Foundation of Pittsburg"}],"content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2009,8]]},"abstract":"<jats:sec>\n            <jats:title\/>\n            <jats:p>Donor-induced and third-party\u2013induced proliferation of T-helper and T-cytotoxic (Tc) cells and their na\u00efve and memory subsets was evaluated simultaneously in single blood samples from 77 children who received steroid-free liver transplantation (LTx) after induction with rabbit anti-human thymocyte globulin. Proliferation was measured by dilution of the intravital dye carboxyfluorescein succinimidyl ester (CFSE) in a 3- to 4-day mixed lymphocyte response coculture. The ratio of donor\/third-party\u2013induced proliferated (CFSE<jats:sup>low<\/jats:sup>) T-cells was reported as the immunoreactivity index (IR) for each subset. Rejectors were defined as those who experienced biopsy-proven acute cellular rejection within 60 days of the assay. IR &gt; 1 signified increased risk of rejection, and IR &lt; 1 implied decreased risk. Demographics for 32 rejectors and 45 nonrejectors were similar. Proliferated CFSE<jats:sup>low<\/jats:sup> T-cells and subsets were significantly higher among rejectors compared with nonrejectors. In 33 of 77 randomly selected children, logistic regression, leave-one-out cross-validation, and receiver operating characteristic analyses showed that the IR of Tc cells was best associated with biopsy-proven rejection (sensitivity &gt; 75%, specificity &gt; 88%). Sensitivity and specificity were replicated in the remaining 44 children who composed the validation cohort. IR of CFSE<jats:sup>low<\/jats:sup> Tc cells correlated significantly with IR of proinflammatory, allospecific CD154+ Tc cells (<jats:italic toggle=\"yes\">r<\/jats:italic> = 0.664, <jats:italic toggle=\"yes\">P<\/jats:italic> = 0.0005) and inversely with IR of allospecific, anti-inflammatory, cytotoxic T lymphocyte antigen 4\u2013positive Tc cells (<jats:italic toggle=\"yes\">r<\/jats:italic> = \u22120.630, <jats:italic toggle=\"yes\">P<\/jats:italic> = 0.007). In conclusion, proliferative alloresponses of Tc cells can identify rejection-prone children receiving LTx. Liver Transpl 15:978\u2013985, 2009. \u00a9 2009 AASLD.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1002\/lt.21775","type":"journal-article","created":{"date-parts":[[2009,7,29]],"date-time":"2009-07-29T15:41:50Z","timestamp":1248882110000},"page":"978-985","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":8,"title":["Proliferative alloresponse of T-cytotoxic cells identifies rejection-prone children with steroid-free liver transplantation"],"prefix":"10.1097","volume":"15","author":[{"given":"Chethan","family":"Ashokkumar","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qing","family":"Sun","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ankit","family":"Gupta","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Brandon 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