{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T23:27:44Z","timestamp":1778628464654,"version":"3.51.4"},"reference-count":37,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"2","license":[{"start":{"date-parts":[[2012,2,1]],"date-time":"2012-02-01T00:00:00Z","timestamp":1328054400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"},{"start":{"date-parts":[[2012,1,25]],"date-time":"2012-01-25T00:00:00Z","timestamp":1327449600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2012,2]]},"abstract":"<jats:sec>\n            <jats:title\/>\n            <jats:p>The increasing shortage of donor organs has led to the increasing use of organs from non\u2013heart-beating donors. We aimed to assess the impact of venous systemic oxygen persufflation (VSOP) supplemented with nitric oxide (NO) gas during the cold storage (CS) of warm ischemia (WI)\u2013damaged experimental liver grafts. Rat livers (n = 5 per group) were retrieved after 30 minutes of WI induced by cardiac arrest (the WI group) and were thereafter preserved for 24 hours by CS in histidine tryptophan ketoglutarate solution. During CS, gaseous oxygen was insufflated via the caval vein with 40 ppm NO (the VSOP-NO group) or without NO (the VSOP group). Cold-stored livers without WI served as controls. Liver viability was assessed after the preservation period by normothermic isolated reperfusion for 45 minutes with oxygenated Krebs-Henseleit buffer. After 45 minutes of reperfusion, the VSOP-NO\u2013treated livers showed significantly lower alanine aminotransferase values than the WI-damaged livers (10.2 \u00b1 0.2 versus 78.2 \u00b1 14.6 IU\/L), whereas the control livers showed no differences from the VSOP-NO\u2013treated livers. The mitochondrial enzyme release was lower in the VSOP-NO group (4.0 \u00b1 0.7 IU\/L) versus the WI group (18.2 \u00b1 4.9 IU\/L). An increased portal vein pressure was observed throughout reperfusion (45 minutes) in the WI group (21.7 \u00b1 0.2 mm Hg) versus the VSOP-NO group (12.2 \u00b1 0.8 mm Hg) and the control group (19.9 \u00b1 0.4 mm Hg). Furthermore, the NO concentration in the perfusate after 5 minutes of reperfusion was highest in the VSOP-NO group. The release of malondialdehyde into the perfusate was significantly reduced in the VSOP-NO group (0.9 \u00b1 0.1 nmol\/mL) versus the WI group (31.3 \u00b1 5.3 nmol\/mL). In conclusion, the resuscitation of livers after 30 minutes of WI to a level comparable to that of nonischemically damaged livers is possible with VSOP supplemented with NO gas. Moreover, the application of VSOP with NO minimizes the extent of injuries caused by oxygen free radicals during preservation. Liver Transpl 18:219\u2013225, 2012. \u00a9 2011 AASLD.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1002\/lt.22442","type":"journal-article","created":{"date-parts":[[2011,10,10]],"date-time":"2011-10-10T19:15:25Z","timestamp":1318274125000},"page":"219-225","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":24,"title":["Impact of venous systemic oxygen persufflation supplemented with nitric oxide gas on cold-stored, warm ischemia\u2013damaged experimental liver grafts"],"prefix":"10.1097","volume":"18","author":[{"given":"Pramod Kadaba","family":"Srinivasan","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shintaro","family":"Yagi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Benedict","family":"Doorschodt","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kazuyuki","family":"Nagai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mamdouh","family":"Afify","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shinji","family":"Uemoto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rene","family":"Tolba","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"276","reference":[{"key":"R1-11-20250124","doi-asserted-by":"crossref","first-page":"1223","DOI":"10.1002\/lt.20268","article-title":"Liver transplantation from nonheartbeating donors: current status and future prospects.","volume":"10","author":"Reddy","year":"2004","journal-title":"Liver Transpl"},{"key":"R2-11-20250124","doi-asserted-by":"crossref","first-page":"844","DOI":"10.1093\/oxfordjournals.bmb.a011652","article-title":"The non heartbeating donor.","volume":"53","author":"Kootstra","year":"1997","journal-title":"Br Med Bull"},{"key":"R3-11-20250124","first-page":"710","article-title":"Liver and kidney transplantation from nonheart beating donors: the Pittsburgh experience.","volume":"27","author":"Casavilla","year":"1995","journal-title":"Transplant Proc"},{"key":"R4-11-20250124","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1080\/08941930600778198","article-title":"Reduced liver apoptosis after venous systemic oxygen persufflation in nonheartbeating donors.","volume":"19","author":"Tolba","year":"2006","journal-title":"J Invest Surg"},{"key":"R5-11-20250124","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1097\/01.sla.0000103063.82181.2c","article-title":"Survival following liver transplantation from nonheartbeating donors.","volume":"239","author":"Abt","year":"2004","journal-title":"Ann Surg"},{"key":"R6-11-20250124","doi-asserted-by":"crossref","first-page":"1659","DOI":"10.1097\/01.TP.0000062574.18648.7C","article-title":"Liver transplantation from controlled nonheartbeating donors: an increased incidence of biliary complications.","volume":"75","author":"Abt","year":"2003","journal-title":"Transplantation"},{"key":"R7-11-20250124","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1016\/j.pathophys.2006.05.008","article-title":"Divergent roles of superoxide and nitric oxide in reducedsize liver ischemia and reperfusion injury: implications for partial liver transplantation.","volume":"13","author":"Urakami","year":"2006","journal-title":"Pathophysiology"},{"key":"R8-11-20250124","doi-asserted-by":"crossref","first-page":"1240","DOI":"10.1172\/JCI113441","article-title":"Reactive oxygen species during ischemiareflow injury in isolated perfused rat liver.","volume":"81","author":"Jaeschke","year":"1988","journal-title":"J Clin Invest"},{"key":"R9-11-20250124","doi-asserted-by":"crossref","first-page":"1223","DOI":"10.1002\/hep.510300513","article-title":"Ischemic preconditioning protects the mouse liver by inhibition of apoptosis through a caspasedependent pathway.","volume":"30","author":"Yadav","year":"1999","journal-title":"Hepatology"},{"key":"R10-11-20250124","doi-asserted-by":"crossref","first-page":"168","DOI":"10.1016\/S0016-5085(99)70241-6","article-title":"Calpain mediates ischemic injury of the liver through modulation of apoptosis and necrosis.","volume":"116","author":"Kohli","year":"1999","journal-title":"Gastroenterology"},{"key":"R11-11-20250124","doi-asserted-by":"crossref","first-page":"9354","DOI":"10.1073\/pnas.94.17.9354","article-title":"Calpain is a mediator of preservationreperfusion injury in rat liver transplantation.","volume":"94","author":"Kohli","year":"1997","journal-title":"Proc Natl Acad Sci U S A"},{"key":"R12-11-20250124","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1038\/sj.bjp.0707224","article-title":"Recent developments in nitric oxide donor drugs.","volume":"151","author":"Miller","year":"2007","journal-title":"Br J Pharmacol"},{"key":"R13-11-20250124","doi-asserted-by":"crossref","first-page":"148","DOI":"10.1097\/MOT.0b013e3282f63930","article-title":"Liver preservation: is there anything new yet?","volume":"13","author":"Guarrera","year":"2008","journal-title":"Curr Opin Organ Transplant"},{"key":"R14-11-20250124","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1080\/08941930802709470","article-title":"Nitric oxide mechanism of protection in ischemia and reperfusion injury.","volume":"22","author":"Phillips","year":"2009","journal-title":"J Invest Surg"},{"key":"R15-11-20250124","doi-asserted-by":"crossref","first-page":"688","DOI":"10.1002\/hep.20089","article-title":"Ischemic preconditioning affects interleukin release in fatty livers of rats undergoing ischemiareperfusion.","volume":"39","author":"Serafin","year":"2004","journal-title":"Hepatology"},{"key":"R16-11-20250124","doi-asserted-by":"crossref","first-page":"587","DOI":"10.1016\/S0002-9440(10)64214-9","article-title":"Ischemic preconditioning increases the tolerance of fatty liver to hepatic ischemiareperfusion injury in the rat.","volume":"161","author":"Serafin","year":"2002","journal-title":"Am J Pathol"},{"key":"R17-11-20250124","doi-asserted-by":"crossref","first-page":"2583","DOI":"10.1172\/JCI31892","article-title":"Inhaled NO accelerates restoration of liver function in adults following orthotopic liver transplantation.","volume":"117","author":"Lang","year":"2007","journal-title":"J Clin Invest"},{"issue":"pt 1","key":"R18-11-20250124","doi-asserted-by":"crossref","first-page":"454","DOI":"10.1164\/ajrccm.156.2.9608007","article-title":"Effects of various timings and concentrations of inhaled nitric oxide in lung ischemiareperfusion. The ParisSud University Lung Transplantation Group.","volume":"156","author":"Murakami","year":"1997","journal-title":"Am J Respir Crit Care Med"},{"key":"R19-11-20250124","doi-asserted-by":"crossref","first-page":"2707","DOI":"10.1111\/j.1600-6143.2009.02840.x","article-title":"Nitric oxide ventilation of rat lungs from nonheartbeating donors improves posttransplant function.","volume":"9","author":"Dong","year":"2009","journal-title":"Am J Transplant"},{"key":"R20-11-20250124","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1016\/S0168-8278(00)80196-8","article-title":"Cold preservation of fatty liver grafts: prevention of functional and ultrastructural impairments by venous oxygen persufflation.","volume":"32","author":"Minor","year":"2000","journal-title":"J Hepatol"},{"key":"R21-11-20250124","doi-asserted-by":"crossref","first-page":"333","DOI":"10.1159\/000129225","article-title":"Evaluation of antioxidant treatment with superoxide dismutase in rat liver transplantation after warm ischemia.","volume":"24","author":"Minor","year":"1992","journal-title":"Eur Surg Res"},{"key":"R22-11-20250124","doi-asserted-by":"crossref","first-page":"313","DOI":"10.3109\/10520296009114754","article-title":"Embedding in epoxy resins for ultrathin sectioning in electron microscopy.","volume":"35","author":"Richardson","year":"1960","journal-title":"Stain Technol"},{"key":"R23-11-20250124","doi-asserted-by":"crossref","first-page":"917","DOI":"10.1097\/00007890-199704150-00001","article-title":"The asystolic, or nonheartbeating, donor.","volume":"63","author":"Kootstra","year":"1997","journal-title":"Transplantation"},{"key":"R24-11-20250124","first-page":"2913","article-title":"Time is of the essence: the pressing need for comprehensive nonheartbeating cadaveric donation policies.","volume":"27","author":"Arnold","year":"1995","journal-title":"Transplant Proc"},{"key":"R25-11-20250124","doi-asserted-by":"crossref","first-page":"1068","DOI":"10.1097\/01.TP.0000085043.78445.53","article-title":"Liver transplantation from Maastricht category 2 nonheartbeating donors.","volume":"76","author":"Otero","year":"2003","journal-title":"Transplantation"},{"key":"R26-11-20250124","doi-asserted-by":"crossref","first-page":"506","DOI":"10.1097\/00007890-198810000-00007","article-title":"Reliable indices for the determination of viability of grafted liver immediately after orthotopic transplantation. Bile flow rate and cellular adenosine triphosphate level.","volume":"46","author":"Sumimoto","year":"1988","journal-title":"Transplantation"},{"key":"R27-11-20250124","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1007\/BF00587833","article-title":"Metabolism of canine kidneys in anaerobic ischemia and in aerobic ischemia by persufflation with gaseous oxygen.","volume":"337","author":"Isselhard","year":"1972","journal-title":"Pflugers Arch"},{"key":"R28-11-20250124","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1007\/BF01851149","article-title":"Renal function after hypothermic kidney ischemia with orthograde and retrograde O 2 persulflation in situ in German.","volume":"157","author":"Isselhard","year":"1972","journal-title":"Res Exp Med (Berl)"},{"key":"R29-11-20250124","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1097\/00007890-199407000-00027","article-title":"Venous oxygen insufflation to prevent reoxygenation injury after ischemia of a solid organ.","volume":"58","author":"Minor","year":"1994","journal-title":"Transplantation"},{"key":"R30-11-20250124","doi-asserted-by":"crossref","first-page":"728","DOI":"10.1007\/BF00311713","article-title":"The effects of allopurinol and SOD on lipid peroxidation and energy metabolism in the liver after ischemia in an aerobicanaerobic persufflation.","volume":"23","author":"Minor","year":"1993","journal-title":"Surg Today"},{"key":"R31-11-20250124","doi-asserted-by":"crossref","first-page":"182","DOI":"10.1006\/cryo.2000.2231","article-title":"Gaseous oxygen for hypothermic preservation of predamaged liver grafts: fuel to cellular homeostasis or radical tissue alteration?","volume":"40","author":"Minor","year":"2000","journal-title":"Cryobiology"},{"key":"R32-11-20250124","first-page":"258","article-title":"Short time kidney preservation by hypothermic oxygen persufflation.","volume":"31","author":"Sachweh","year":"1972","journal-title":"Bull Soc Int Chir"},{"key":"R33-11-20250124","doi-asserted-by":"crossref","first-page":"1843","DOI":"10.1097\/00007890-199706270-00025","article-title":"Biophysical aspects of liver aeration by vascular persufflation with gaseous oxygen.","volume":"63","author":"Minor","year":"1997","journal-title":"Transplantation"},{"key":"RU34-11-20250124","doi-asserted-by":"crossref","first-page":"361","DOI":"10.1055\/s-0037-1613489","article-title":"Fibrinolysis in organ procurement for transplantation after cardiocirculatory compromise.","volume":"90","author":"Minor","year":"2003","journal-title":"Thromb Haemost"},{"key":"R35-11-20250124","first-page":"45","article-title":"Microcirculatory disturbances and leucocyte adherence in transplanted livers after cold storage in EuroCollins, UW and HTK solutions.","volume":"4","author":"Marzi","year":"1991","journal-title":"Transpl Int"},{"key":"R36-11-20250124","doi-asserted-by":"crossref","first-page":"579","DOI":"10.1097\/00007890-199809150-00005","article-title":"Beneficial effects of FK409, a novel nitric oxide donor, on reperfusion injury of rat liver.","volume":"66","author":"Ohmori","year":"1998","journal-title":"Transplantation"},{"key":"R37-11-20250124","doi-asserted-by":"crossref","first-page":"1681","DOI":"10.1097\/01.TP.0000093832.15249.AD","article-title":"Lcarnitine ameliorates abnormal vulnerability of steatotic rat livers to cold ischemic preservation.","volume":"76","author":"Tolba","year":"2003","journal-title":"Transplantation"}],"container-title":["Liver Transplantation"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Flt.22442","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Flt.22442","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.lww.com\/01445473-201202000-00011","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,1,24]],"date-time":"2025-01-24T17:12:55Z","timestamp":1737738775000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.lww.com\/01445473-201202000-00011"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,2]]},"references-count":37,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2012]]}},"URL":"https:\/\/doi.org\/10.1002\/lt.22442","relation":{},"ISSN":["1527-6465"],"issn-type":[{"value":"1527-6465","type":"print"}],"subject":[],"published":{"date-parts":[[2012,2]]}}}