{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T21:13:49Z","timestamp":1777497229371,"version":"3.51.4"},"reference-count":43,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"8","license":[{"start":{"date-parts":[[2014,8,1]],"date-time":"2014-08-01T00:00:00Z","timestamp":1406851200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"},{"start":{"date-parts":[[2014,7,2]],"date-time":"2014-07-02T00:00:00Z","timestamp":1404259200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Ohio Solid Organ Transplantation Consortium","award":["OSOTC1204CQ"],"award-info":[{"award-number":["OSOTC1204CQ"]}]}],"content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2014,8]]},"abstract":"<jats:sec>\n            <jats:title\/>\n            <jats:p>The effects of normothermic machine perfusion (NMP) on the postreperfusion hemodynamics and extrahepatic biliary duct histology of donation after cardiac death (DCD) livers after transplantation have not been addressed thoroughly and represent the objective of this study. Ten livers (5 per group) with 60 minutes of warm ischemia were preserved via cold storage (CS) or sanguineous NMP for 10 hours, and then they were reperfused for 24 hours with whole blood in an isolated perfusion system to simulate transplantation. In our experiment, the arterial and portal vein flows were stable in the NMP group during the entire reperfusion simulation, whereas they decreased dramatically in the CS group after 16 hours of reperfusion (<jats:italic toggle=\"yes\">P<\/jats:italic> &lt; 0.05); these findings were consistent with severe parenchymal injury. Similarly, significant differences existed between the CS and NMP groups with respect to the release of hepatocellular enzymes, the volume of bile produced, and the levels of enzymes released into bile (<jats:italic toggle=\"yes\">P<\/jats:italic> &lt; 0.05). According to histology, CS livers presented with diffuse hepatocyte congestion, necrosis, intraparenchymal hemorrhaging, denudated biliary epithelium, and submucosal bile duct necrosis, whereas NMP livers showed very mild injury to the liver parenchyma and biliary architecture. Most importantly, Ki-67 staining in extrahepatic bile ducts showed biliary epithelial regeneration. In conclusion, our findings advance the knowledge of the postreperfusion events that characterize DCD livers and suggest NMP as a beneficial preservation modality that is able to improve biliary regeneration after a major ischemic event and may prevent the development of ischemic cholangiopathy in the setting of clinical transplantation. <jats:italic toggle=\"yes\">Liver Transpl 20:987\u2013999, 2014<\/jats:italic>. \u00a9 2014 AASLD.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1002\/lt.23906","type":"journal-article","created":{"date-parts":[[2014,5,8]],"date-time":"2014-05-08T09:47:10Z","timestamp":1399542430000},"page":"987-999","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":99,"title":["Sanguineous normothermic machine perfusion improves hemodynamics and biliary epithelial regeneration in donation after cardiac death porcine livers"],"prefix":"10.1097","volume":"20","author":[{"given":"Qiang","family":"Liu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ahmed","family":"Nassar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kevin","family":"Farias","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laura","family":"Buccini","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"William","family":"Baldwin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martin","family":"Mangino","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana","family":"Bennett","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Colin","family":"O'Rourke","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Toshiro","family":"Okamoto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Teresa Diago","family":"Uso","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John","family":"Fung","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kareem","family":"Abu-Elmagd","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Charles","family":"Miller","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cristiano","family":"Quintini","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"276","reference":[{"issue":"pt 2","key":"R1-17-20250124","doi-asserted-by":"crossref","first-page":"961","DOI":"10.1111\/j.1600-6143.2010.03021.x","article-title":"Trends in organ donation and transplantation in the United States, 1999-2008.","volume":"10","author":"Wolfe","year":"2010","journal-title":"Am J Transplant"},{"key":"R2-17-20250124","doi-asserted-by":"crossref","first-page":"1694","DOI":"10.1002\/lt.21668","article-title":"Report of the Paris consensus meeting on expanded criteria donors in liver transplantation.","volume":"14","author":"Durand","year":"2008","journal-title":"Liver Transpl"},{"key":"R3-17-20250124","doi-asserted-by":"crossref","first-page":"1223","DOI":"10.1002\/lt.20268","article-title":"Liver transplantation from nonheartbeating donors: current status and future prospects.","volume":"10","author":"Reddy","year":"2004","journal-title":"Liver Transpl"},{"key":"R4-17-20250124","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1111\/j.1432-2277.2012.01550.x","article-title":"Is there an advantage of living over deceased donation in liver transplantation?","volume":"26","author":"Quintini","year":"2013","journal-title":"Transpl Int"},{"key":"R6-17-20250124","doi-asserted-by":"crossref","first-page":"817","DOI":"10.1097\/SLA.0b013e3182104784","article-title":"Biliary complications after liver transplantation from donation after cardiac death donors: an analysis of risk factors and longterm outcomes from a single center.","volume":"253","author":"Foley","year":"2011","journal-title":"Ann Surg"},{"key":"R7-17-20250124","doi-asserted-by":"crossref","first-page":"164329","DOI":"10.1155\/2012\/164329","article-title":"Ischemiareperfusion injury and ischemictype biliary lesions following liver transplantation.","volume":"2012","author":"Cursio","year":"2012","journal-title":"J Transplant"},{"key":"R8-17-20250124","doi-asserted-by":"crossref","first-page":"1065","DOI":"10.1016\/j.jhep.2013.02.023","article-title":"The origin of biliary strictures after liver transplantation: is it the amount of epithelial injury or insufficient regeneration that counts?","volume":"58","author":"Karimian","year":"2013","journal-title":"J Hepatol"},{"key":"R9-17-20250124","doi-asserted-by":"crossref","first-page":"114","DOI":"10.1097\/00000658-200101000-00017","article-title":"Liver transplantation after organ preservation with normothermic extracorporeal perfusion.","volume":"233","author":"Schon","year":"2001","journal-title":"Ann Surg"},{"key":"R10-17-20250124","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1097\/SLA.0b013e3181a63c10","article-title":"Normothermic perfusion: a new paradigm for organ preservation.","volume":"250","author":"Brockmann","year":"2009","journal-title":"Ann Surg"},{"key":"R11-17-20250124","doi-asserted-by":"crossref","first-page":"1000","DOI":"10.1097\/SLA.0b013e31822b8b2f","article-title":"Superior preservation of DCD livers with continuous normothermic perfusion.","volume":"254","author":"Fondevila","year":"2011","journal-title":"Ann Surg"},{"key":"R12-17-20250124","doi-asserted-by":"crossref","first-page":"701","DOI":"10.1097\/00007890-200203150-00008","article-title":"Advantages of normothermic perfusion over cold storage in liver preservation.","volume":"73","author":"Imber","year":"2002","journal-title":"Transplantation"},{"key":"R13-17-20250124","doi-asserted-by":"crossref","first-page":"e83","DOI":"10.1016\/j.jss.2011.09.057","article-title":"Excorporeal normothermic machine perfusion resuscitates pig DCD livers with extended warm ischemia.","volume":"173","author":"Xu","year":"2012","journal-title":"J Surg Res"},{"key":"R14-17-20250124","first-page":"173","article-title":"Normothermic extracorporeal perfusion of isolated porcine liver after warm ischaemia: a preliminary report.","volume":"14","author":"Bellomo","year":"2012","journal-title":"Crit Care Resusc"},{"key":"R15-17-20250124","doi-asserted-by":"crossref","first-page":"170","DOI":"10.1097\/TP.0b013e318192df6b","article-title":"Recovery of warm ischemic rat liver grafts by normothermic extracorporeal perfusion.","volume":"87","author":"Tolboom","year":"2009","journal-title":"Transplantation"},{"key":"R16-17-20250124","doi-asserted-by":"crossref","first-page":"1441","DOI":"10.1111\/ajt.12224","article-title":"Normothermic acellular ex vivo liver perfusion reduces liver and bile duct injury of pig livers retrieved after cardiac death.","volume":"13","author":"Boehnert","year":"2013","journal-title":"Am J Transplant"},{"key":"R17-17-20250124","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1159\/000100800","article-title":"Flow and pressure during liver preservation under ex situ and in situ perfusion with University of Wisconsin solution and histidinetryptophanketoglutarate solution.","volume":"39","author":"Moench","year":"2007","journal-title":"Eur Surg Res"},{"key":"R18-17-20250124","doi-asserted-by":"crossref","first-page":"365","DOI":"10.1002\/lt.21372","article-title":"Comparison of histidinetryptophanketoglutarate solution and University of Wisconsin solution in extended criteria liver donors.","volume":"14","author":"Mangus","year":"2008","journal-title":"Liver Transpl"},{"key":"R19-17-20250124","doi-asserted-by":"crossref","first-page":"1125","DOI":"10.1002\/lt.21208","article-title":"Histidinetryptophanketoglutarate solution vs. University of Wisconsin solution for liver transplantation: a systematic review.","volume":"13","author":"Feng","year":"2007","journal-title":"Liver Transpl"},{"key":"R20-17-20250124","doi-asserted-by":"crossref","first-page":"1212","DOI":"10.1097\/00007890-200204270-00005","article-title":"Successful extracorporeal porcine liver perfusion for 72 hr.","volume":"73","author":"Butler","year":"2002","journal-title":"Transplantation"},{"key":"R21-17-20250124","doi-asserted-by":"crossref","first-page":"1327","DOI":"10.1111\/ajt.12187","article-title":"Ex vivo normothermic machine perfusion and viability testing of discarded human donor livers.","volume":"13","author":"op den Dries","year":"2013","journal-title":"Am J Transplant"},{"key":"R22-17-20250124","doi-asserted-by":"crossref","first-page":"6046","DOI":"10.3748\/wjg.v16.i48.6046","article-title":"Regulation of hepatic blood flow: the hepatic arterial buffer response revisited.","volume":"16","author":"Eipel","year":"2010","journal-title":"World J Gastroenterol"},{"key":"R23-17-20250124","doi-asserted-by":"crossref","first-page":"278","DOI":"10.1016\/j.jhep.2012.10.004","article-title":"Protective mechanisms of endischemic cold machine perfusion in DCD liver grafts.","volume":"58","author":"Schlegel","year":"2013","journal-title":"J Hepatol"},{"key":"R24-17-20250124","doi-asserted-by":"crossref","first-page":"606","DOI":"10.1046\/j.1365-2168.2002.02052.x","article-title":"Extended preservation of nonheartbeating donor livers with normothermic machine perfusion.","volume":"89","author":"St Peter","year":"2002","journal-title":"Br J Surg"},{"key":"R25-17-20250124","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1006\/cryo.2000.2276","article-title":"Bile analysis as a tool for assessing integrity of biliary epithelial cells after cold ischemiareperfusion of rat livers.","volume":"41","author":"Vajdova","year":"2000","journal-title":"Cryobiology"},{"key":"R26-17-20250124","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1007\/s00428-012-1245-8","article-title":"Histological examination and evaluation of donor bile ducts received during orthotopic liver transplantationa morphological clue to ischemictype biliary lesion?","volume":"461","author":"Hansen","year":"2012","journal-title":"Virchows Arch"},{"key":"R28-17-20250124","doi-asserted-by":"crossref","first-page":"554","DOI":"10.1111\/j.1478-3231.2011.02721.x","article-title":"Regeneration of human extrahepatic biliary epithelium: the peribiliary glands as progenitor cell compartment.","volume":"32","author":"Sutton","year":"2012","journal-title":"Liver Int"},{"key":"R29-17-20250124","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1055\/s-0031-1300952","article-title":"New insights into von Willebrand disease and platelet function.","volume":"38","author":"Szanto","year":"2012","journal-title":"Semin Thromb Hemost"},{"key":"R30-17-20250124","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1097\/00007691-200104000-00001","article-title":"The MEGX test: a tool for the realtime assessment of hepatic function.","volume":"23","author":"Oellerich","year":"2001","journal-title":"Ther Drug Monit"},{"key":"R31-17-20250124","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1097\/MOT.0b013e328337349d","article-title":"Exvivo normothermic liver perfusion: an update.","volume":"15","author":"Vogel","year":"2010","journal-title":"Curr Opin Organ Transplant"},{"key":"R32-17-20250124","doi-asserted-by":"crossref","first-page":"2463","DOI":"10.1016\/j.transproceed.2010.04.037","article-title":"Diluted blood reperfusion as a model for transplantation of ischemic rat livers: alanine aminotransferase is a direct indicator of viability.","volume":"42","author":"Uygun","year":"2010","journal-title":"Transplant Proc"},{"key":"R33-17-20250124","doi-asserted-by":"crossref","first-page":"708","DOI":"10.1002\/lt.21166","article-title":"Nonanastomotic biliary strictures after liver transplantation, part 1: radiological features and risk factors for early vs late presentation.","volume":"13","author":"Buis","year":"2007","journal-title":"Liver Transpl"},{"key":"R34-17-20250124","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1016\/j.jss.2007.06.015","article-title":"Factors in the pathophysiology of the liver ischemiareperfusion injury.","volume":"147","author":"MontalvoJave","year":"2008","journal-title":"J Surg Res"},{"key":"R35-17-20250124","doi-asserted-by":"crossref","first-page":"1448","DOI":"10.1213\/00000539-200206000-00012","article-title":"New insights into the regulation of hepatic blood flow after ischemia and reperfusion.","volume":"94","author":"Pannen","year":"2002","journal-title":"Anesth Analg"},{"key":"R36-17-20250124","doi-asserted-by":"crossref","first-page":"G1219","DOI":"10.1152\/ajpgi.00527.2007","article-title":"Differential mechanisms of hepatic vascular dysregulation with mild vs. moderate ischemiareperfusion.","volume":"294","author":"Lee","year":"2008","journal-title":"Am J Physiol Gastrointest Liver Physiol"},{"key":"R37-17-20250124","doi-asserted-by":"crossref","first-page":"6308","DOI":"10.3748\/wjg.v18.i43.6308","article-title":"Effects of warm ischemia time on biliary injury in rat liver transplantation.","volume":"18","author":"Zhu","year":"2012","journal-title":"World J Gastroenterol"},{"key":"R38-17-20250124","doi-asserted-by":"crossref","first-page":"3392","DOI":"10.1016\/j.transproceed.2010.07.099","article-title":"Biliary complications after orthotopic liver transplantation from donors after cardiac death: broad spectrum of disease.","volume":"42","author":"Abou Abbass","year":"2010","journal-title":"Transplant Proc"},{"key":"R39-17-20250124","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1111\/j.1440-1746.1994.tb01220.x","article-title":"Intrahepatic peribiliary glands of humans. I. Anatomy, development and presumed functions.","volume":"9","author":"Nakanuma","year":"1994","journal-title":"J Gastroenterol Hepatol"},{"key":"R40-17-20250124","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1038\/nrgastro.2012.23","article-title":"The biliary treea reservoir of multipotent stem cells.","volume":"9","author":"Cardinale","year":"2012","journal-title":"Nat Rev Gastroenterol Hepatol"},{"key":"R41-17-20250124","doi-asserted-by":"crossref","first-page":"803","DOI":"10.1002\/hep.1840180409","article-title":"Extrahepatic peribiliary glands express alphaamylase isozymes, trypsin and pancreatic lipase: an immunohistochemical analysis.","volume":"18","author":"Terada","year":"1993","journal-title":"Hepatology"},{"key":"R42-17-20250124","doi-asserted-by":"crossref","first-page":"1236","DOI":"10.1016\/0016-5085(95)90225-2","article-title":"Expression of pancreatic enzymes (alphaamylase, trypsinogen, and lipase) during human liver development and maturation.","volume":"108","author":"Terada","year":"1995","journal-title":"Gastroenterology"},{"key":"R43-17-20250124","doi-asserted-by":"crossref","first-page":"G469","DOI":"10.1152\/ajpgi.00407.2012","article-title":"Hepatic sinusoidal endothelium avidly binds platelets in an integrindependent manner, leading to platelet and endothelial activation and leukocyte recruitment.","volume":"304","author":"Lalor","year":"2013","journal-title":"Am J Physiol Gastrointest Liver Physiol"},{"key":"R44-17-20250124","doi-asserted-by":"crossref","first-page":"1328","DOI":"10.1097\/01.TP.0000119206.63326.56","article-title":"Preservation of porcine nonheartbeating donor livers by sequential cold storage and warm perfusion.","volume":"77","author":"Reddy","year":"2004","journal-title":"Transplantation"},{"key":"R45-17-20250124","first-page":"177","article-title":"Comparison of histidinetryptophanketoglutarate (HTK) solution versus University of Wisconsin (UW) solution for organ preservation in human liver transplantation. A prospective, randomized study.","volume":"7","author":"Erhard","year":"1994","journal-title":"Transpl Int"}],"container-title":["Liver Transplantation"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Flt.23906","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Flt.23906","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.lww.com\/01445473-201408000-00017","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,1,24]],"date-time":"2025-01-24T17:30:07Z","timestamp":1737739807000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.lww.com\/01445473-201408000-00017"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,8]]},"references-count":43,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2014]]}},"URL":"https:\/\/doi.org\/10.1002\/lt.23906","relation":{},"ISSN":["1527-6465"],"issn-type":[{"value":"1527-6465","type":"print"}],"subject":[],"published":{"date-parts":[[2014,8]]}}}