{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,24]],"date-time":"2026-06-24T15:48:04Z","timestamp":1782316084238,"version":"3.54.5"},"reference-count":30,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"7","license":[{"start":{"date-parts":[[2015,9,1]],"date-time":"2015-09-01T00:00:00Z","timestamp":1441065600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Liver Transpl"],"published-print":{"date-parts":[[2016,7]]},"abstract":"<jats:p>The purpose of this study is to explore whether normothermic machine perfusion (NMP) preservation is superior to cold preservation during reduced\u2010size liver transplantation (RSLT) in pigs. Twenty\u2010four healthy Ba\u2010Ma mini pigs were used (aged &gt;13 months; weight 25\u201035 kg; regardless of sex). The animals were randomized into 2 groups. In group A (NMP), donor livers were harvested without warm ischemia time and heartbeats and then were connected to the NMP system to reduce the livers' size under the normothermic condition. In group B (University of Wisconsin [UW] solution), donor livers were harvested without warm ischemia time and heartbeats after being perfused by UW solution and were then preserved in 0\u00b0C\u20104\u00b0C UW solution to reduce the livers' size under cold conditions. After that, liver transplantation without venovenous bypass was performed. General RSLT information of the pigs from the 2 groups was recorded; the serological indices were measured; and routine pathological examination of liver tissue was observed. A significant difference was observed in the intraoperative bleeding between the 2 groups (<jats:italic toggle=\"yes\">P<\/jats:italic> &lt; 0.05), whereas no significant difference was found in the other indices (all <jats:italic toggle=\"yes\">P<\/jats:italic> &gt; 0.05). Significant differences of alanine aminotransferase levels, aspartate aminotransferase levels, and lactate dehydrogenase levels between the 2 groups were observed between postoperative days 3 and 5 (<jats:italic toggle=\"yes\">P<\/jats:italic> &lt; 0.05). Significant differences of lactic acid levels between the 2 groups were observed between postoperative days 2 and 5 (<jats:italic toggle=\"yes\">P<\/jats:italic> &lt; 0.05). Compared with the cold preservation group, the liver tissues of the NMP preservation group only rarely experienced liver cell necrosis and maintained integrities in the hepatic sinusoid spaces and endothelial cells. In conclusion, NMP preservation is superior to cold preservation during RSLT in pigs. <jats:italic toggle=\"yes\">Liver Transplantation 22 968\u2013978 2016 AASLD<\/jats:italic>\n                        <\/jats:p>","DOI":"10.1002\/lt.24453","type":"journal-article","created":{"date-parts":[[2016,4,2]],"date-time":"2016-04-02T16:36:01Z","timestamp":1459614961000},"page":"968-978","source":"Crossref","is-referenced-by-count":17,"title":["Protective role of normothermic machine perfusion during reduced\u2010size liver transplantation in pigs"],"prefix":"10.1097","volume":"22","author":[{"given":"Zhi\u2010Bin","family":"Zhang","sequence":"first","affiliation":[{"name":"Departments of General Surgery,Tianjin First Central Hospital,Tianjin,China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Gao","sequence":"additional","affiliation":[{"name":"Transplantation Surgery,Tianjin First Central Hospital,Tianjin,China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuan","family":"Shi","sequence":"additional","affiliation":[{"name":"Hepatopancreatobiliary Surgery,Tianjin First Central Hospital,Tianjin,China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lei","family":"Liu","sequence":"additional","affiliation":[{"name":"Transplantation Surgery,Tianjin First Central Hospital,Tianjin,China"},{"name":"Tianjin Key Laboratory of Organ Transplantation,Tianjin,China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ning","family":"Ma","sequence":"additional","affiliation":[{"name":"Transplantation Surgery,Tianjin First Central Hospital,Tianjin,China"},{"name":"Tianjin Key Laboratory of Organ Transplantation,Tianjin,China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Chen","sequence":"additional","affiliation":[{"name":"Tianjin Key Laboratory of Organ Transplantation,Tianjin,China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhi\u2010Jun","family":"Zhu","sequence":"additional","affiliation":[{"name":"Department of Transplantation Surgery,Beijing Friendship Hospital,Beijing,China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","published-online":{"date-parts":[[2016,6,24]]},"reference":[{"key":"lt24453-bib-0001-20240115","doi-asserted-by":"crossref","first-page":"417","DOI":"10.1016\/j.cld.2007.04.004","article-title":"Expanding donor options: marginal, living, and split donors","volume":"11","year":"2007","journal-title":"Clin liver Dis"},{"key":"lt24453-bib-0002-20240115","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1055\/s-2006-947292","article-title":"Extended\u2010donor criteria liver allografts","volume":"26","year":"2006","journal-title":"Semin Liver Dis"},{"key":"lt24453-bib-0003-20240115","doi-asserted-by":"crossref","first-page":"1575","DOI":"10.1007\/s00268-009-0139-7","article-title":"Russell Strong and the history of reduced\u2010size liver transplantation","volume":"33","year":"2009","journal-title":"World J Surg"},{"key":"lt24453-bib-0004-20240115","first-page":"1953","article-title":"Reduced\u2010size liver transplantation in children and adults","volume":"23","year":"1991","journal-title":"Transplant Proc"},{"key":"lt24453-bib-0005-20240115","doi-asserted-by":"crossref","first-page":"805","DOI":"10.1016\/S0140-6736(02)09920-8","article-title":"Development of modern liver surgery","volume":"360","year":"2002","journal-title":"Lancet"},{"key":"lt24453-bib-0006-20240115","first-page":"269","article-title":"The case for and technique of heterotopic liver grafting","volume":"11","year":"1979","journal-title":"Transplant Proc"},{"key":"lt24453-bib-0007-20240115","first-page":"367","article-title":"Reduced\u2010sized orthotopic liver graft in hepatic transplantation in children","volume":"95","year":"1984","journal-title":"Surgery"},{"key":"lt24453-bib-0008-20240115","doi-asserted-by":"crossref","first-page":"387","DOI":"10.1097\/00007890-199202010-00023","article-title":"The results of reduced\u2010size liver transplantation, including split livers, in patients with end\u2010stage liver disease","volume":"53","year":"1992","journal-title":"Transplantation"},{"key":"lt24453-bib-0009-20240115","doi-asserted-by":"crossref","first-page":"1130","DOI":"10.1097\/00007890-199311000-00016","article-title":"Impact of innovative techniques on the waiting list and results in pediatric liver transplantation","volume":"56","year":"1993","journal-title":"Transplantation"},{"key":"lt24453-bib-0010-20240115","first-page":"110","article-title":"Graft conditioning of liver in non\u2010heart\u2010beating donors by an artificial heart and lung machine in situ","volume":"28","year":"1996","journal-title":"Transplant Proc"},{"key":"lt24453-bib-0011-20240115","doi-asserted-by":"crossref","first-page":"609","DOI":"10.1046\/j.1365-2168.2002.02052.x","article-title":"Extended preservation of non\u2010heart\u2010beating donor livers with normothermic machine perfusion","volume":"89","year":"2002","journal-title":"Br J Surg"},{"key":"lt24453-bib-0012-20240115","doi-asserted-by":"crossref","first-page":"114","DOI":"10.1097\/00000658-200101000-00017","article-title":"Liver transplantation after organ preservation with normothermic extracorporeal perfusion","volume":"233","year":"2001","journal-title":"Ann Surg"},{"key":"lt24453-bib-0013-20240115","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1097\/SLA.0b013e3181a63c10","article-title":"Normothermic perfusion: a new paradigm for organ preservation","volume":"250","year":"2009","journal-title":"Ann Surg"},{"key":"lt24453-bib-0014-20240115","doi-asserted-by":"crossref","first-page":"946","DOI":"10.1016\/j.transproceed.2012.01.078","article-title":"Functional recovery of donation after cardiac death liver graft by continuous machine perfusion preservation in pigs","volume":"44","year":"2012","journal-title":"Transplant Proc"},{"key":"lt24453-bib-0015-20240115","doi-asserted-by":"crossref","first-page":"1327","DOI":"10.1111\/ajt.12187","article-title":"Ex vivo normothermic machine perfusion and viability testing of discarded human donor livers","volume":"13","year":"2013","journal-title":"Am J Transplant"},{"key":"lt24453-bib-0016-20240115","doi-asserted-by":"crossref","first-page":"1000","DOI":"10.1097\/SLA.0b013e31822b8b2f","article-title":"Superior preservation of DCD livers with continuous normothermic perfusion","volume":"254","year":"2011","journal-title":"Ann Surg"},{"key":"lt24453-bib-0017-20240115","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1016\/S0168-1591(02)00288-5","article-title":"Guidelines for the ethical use of animals in applied ethology studies","volume":"81","year":"2003","journal-title":"Appl Anim Behav Sci"},{"key":"lt24453-bib-0018-20240115","doi-asserted-by":"crossref","first-page":"287","DOI":"10.1016\/S0003-3472(12)00550-7","article-title":"Guidelines for the treatment of animals in behavioural research and teaching","volume":"85","year":"2013","journal-title":"Anim Behav"},{"key":"lt24453-bib-0019-20240115","doi-asserted-by":"crossref","first-page":"364","DOI":"10.1002\/lt.21989","article-title":"Portal hyperperfusion mechanism of injury and stimulus for regeneration in porcine small\u2010for\u2010size transplantation","volume":"16","year":"2010","journal-title":"Liver Transpl"},{"key":"lt24453-bib-0020-20240115","first-page":"659","article-title":"Homotransplantation of the liver in humans","volume":"117","year":"1963","journal-title":"Surg Gynecol Obstet"},{"key":"lt24453-bib-0021-20240115","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1016\/S0039-6060(99)70236-9","article-title":"Innovative techniques for and results of portal vein reconstruction in living\u2010related liver transplantation","volume":"125","year":"1999","journal-title":"Surgery"},{"key":"lt24453-bib-0022-20240115","first-page":"97","article-title":"New techniques for liver transplantation: reduced\u2010size, split\u2010liver, living\u2010related and auxiliary liver transplantation","volume":"208","year":"1995","journal-title":"Scand J Gastroenterol Suppl"},{"key":"lt24453-bib-0023-20240115","doi-asserted-by":"crossref","first-page":"1736","DOI":"10.1016\/j.transproceed.2010.11.031","article-title":"Split liver transplantation","volume":"43","year":"2011","journal-title":"Transplant Proc"},{"key":"lt24453-bib-0024-20240115","doi-asserted-by":"crossref","first-page":"884","DOI":"10.1016\/j.transproceed.2011.02.036","article-title":"Split liver transplantation: an overview","volume":"43","year":"2011","journal-title":"Transplant Proc"},{"key":"lt24453-bib-0025-20240115","doi-asserted-by":"crossref","first-page":"701","DOI":"10.1097\/00007890-200203150-00008","article-title":"Advantages of normothermic perfusion over cold storage in liver preservation","volume":"73","year":"2002","journal-title":"Transplantation"},{"key":"lt24453-bib-0026-20240115","doi-asserted-by":"crossref","first-page":"1212","DOI":"10.1097\/00007890-200204270-00005","article-title":"Successful extracorporeal porcine liver perfusion for 72 hr","volume":"73","year":"2002","journal-title":"Transplantation"},{"key":"lt24453-bib-0027-20240115","doi-asserted-by":"crossref","first-page":"1441","DOI":"10.1111\/ajt.12224","article-title":"Normothermic acellular ex vivo liver perfusion reduces liver and bile duct injury of pig livers retrieved after cardiac death","volume":"13","year":"2013","journal-title":"Am J Transplant"},{"key":"lt24453-bib-0028-20240115","doi-asserted-by":"crossref","first-page":"442","DOI":"10.1111\/j.1445-2197.1997.tb02011.x","article-title":"Hypothermic ischaemia of the liver: a re\u2010perfusion phenomenon","volume":"67","year":"1997","journal-title":"Aust N Z J Surg"},{"key":"lt24453-bib-0029-20240115","doi-asserted-by":"crossref","first-page":"136","DOI":"10.1097\/00007890-197702000-00005","article-title":"Maintenance of the functional state of isolated rat liver by hypothermic perfusion with an erythrocyte\u2010free medium","volume":"23","year":"1977","journal-title":"Transplantation"},{"key":"lt24453-bib-0030-20240115","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1097\/00007890-198501000-00005","article-title":"Correlation between cellular ATP level and bile excretion in the rat liver","volume":"39","year":"1985","journal-title":"Transplantation"}],"container-title":["Liver Transplantation"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Flt.24453","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.lww.com\/01445473-201607000-00014","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,1,15]],"date-time":"2024-01-15T01:08:18Z","timestamp":1705280898000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.lww.com\/01445473-201607000-00014"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,6,24]]},"references-count":30,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2016]]}},"URL":"https:\/\/doi.org\/10.1002\/lt.24453","relation":{},"ISSN":["1527-6465","1527-6473"],"issn-type":[{"value":"1527-6465","type":"print"},{"value":"1527-6473","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,6,24]]}}}