{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T00:43:43Z","timestamp":1790383423924,"version":"4.1.0"},"reference-count":27,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"2","license":[{"start":{"date-parts":[[2017,1,16]],"date-time":"2017-01-16T00:00:00Z","timestamp":1484524800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1"},{"start":{"date-parts":[[2017,1,16]],"date-time":"2017-01-16T00:00:00Z","timestamp":1484524800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Liver Transpl"],"published-print":{"date-parts":[[2017,2]]},"abstract":"<jats:p>Donor organ shortage necessitates use of less than optimal donor allografts for transplantation. The current cold storage preservation technique fails to preserve marginal donor grafts sufficiently. Evidence from large animal experiments suggests superiority of normothermic machine preservation (NMP) of liver allografts. In this study, we analyze discarded human liver grafts that underwent NMP for the extended period of 24 hours. Thirteen human liver grafts which had been discarded for transplantation were entered into this study. Perfusion was performed with an automated device using an oxygenated, sanguineous perfusion solution at normothermia. Automated control was incorporated for temperature\u2010, flow\u2010, and pressure\u2010regulation as well as oxygenation. All livers were perfused for 24 hours; parameters of biochemical and synthetic liver function as well as histological parameters of liver damage were analyzed. Livers were stratified for expected viability according to the donor's medical history, procurement data, and their macroscopic appearance. Normothermic perfusion preservation of human livers for 24 hours was shown to be technically feasible. Human liver grafts, all of which had been discarded for transplantation, showed levels suggesting organ viability with respect to metabolic and synthetic liver function (to varying degrees). There was positive correlation between instantly available perfusion parameters and generally accepted predictors of posttransplant graft survival. In conclusion, NMP is feasible reliably for periods of at least 24 hours, even in highly suboptimal donor organs. Potential benefits include not only viability testing (as suggested in recent clinical implementations), but also removal of the time constraints associated with the utilization of high\u2010risk livers, and recovery of ischemic and other preretrieval injuries (possibly by enabling therapeutic strategies during NMP). <jats:italic toggle=\"yes\">Liver Transplantation 23 207\u2013220 2017 AASLD<\/jats:italic>\n                        <\/jats:p>","DOI":"10.1002\/lt.24672","type":"journal-article","created":{"date-parts":[[2017,1,16]],"date-time":"2017-01-16T08:09:01Z","timestamp":1484554141000},"page":"207-220","source":"Crossref","is-referenced-by-count":95,"title":["The 24\u2010hour normothermic machine perfusion of discarded human liver grafts"],"prefix":"10.1097","volume":"23","author":[{"given":"Thomas","family":"Vogel","sequence":"first","affiliation":[{"name":"Department of General and Visceral Surgery,University Hospital M\u00fcnster,M\u00fcnster,Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jens G.","family":"Brockmann","sequence":"additional","affiliation":[{"name":"King Faisal Specialist Hospital and Research Centre,Riyadh,Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alberto","family":"Quaglia","sequence":"additional","affiliation":[{"name":"Institute of Liver Studies,King's College London,London,UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alireza","family":"Morovat","sequence":"additional","affiliation":[{"name":"Department of Clinical Biochemistry,Oxford University Hospitals National Health Service Trust,Oxford,UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wayel","family":"Jassem","sequence":"additional","affiliation":[{"name":"Institute of Liver Studies,King's College London,London,UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nigel D.","family":"Heaton","sequence":"additional","affiliation":[{"name":"Institute of Liver Studies,King's College London,London,UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Constantin C.","family":"Coussios","sequence":"additional","affiliation":[{"name":"Institute of Biomedical Engineering,University of Oxford,Oxford,UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter J.","family":"Friend","sequence":"additional","affiliation":[{"name":"Nuffield Department of Surgical Sciences,University of Oxford,Oxford,UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","published-online":{"date-parts":[[2017,1,16]]},"reference":[{"key":"lt24672-bib-0001-20240115","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1097\/01.sla.0000103063.82181.2c","article-title":"Survival following liver transplantation from non\u2010heart\u2010beating donors","volume":"239","year":"2004","journal-title":"Ann 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