{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T00:49:15Z","timestamp":1790383755812,"version":"4.1.0"},"reference-count":25,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"4","license":[{"start":{"date-parts":[[2018,3,26]],"date-time":"2018-03-26T00:00:00Z","timestamp":1522022400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Liver Transpl"],"published-print":{"date-parts":[[2018,4]]},"abstract":"<jats:p>Normothermic machine perfusion (NMP) enables viability assessment of donor livers prior to transplantation. NMP is frequently performed by using human blood products including red blood cells (RBCs) and fresh frozen plasma (FFP). Our aim was to examine the efficacy of a novel machine perfusion solution based on polymerized bovine hemoglobin\u2010based oxygen carrier (HBOC)\u2010201. Twenty\u2010four livers declined for transplantation were transported by using static cold storage. Upon arrival, livers underwent NMP for 6 hours using pressure\u2010controlled portal and arterial perfusion. A total of 12 livers were perfused using a solution based on RBCs and FFPs (historical cohort), 6 livers with HBOC\u2010201 and FFPs, and another 6 livers with HBOC\u2010201 and gelofusine, a gelatin\u2010based colloid solution. Compared with RBC + FFP perfused livers, livers perfused with HBOC\u2010201 had significantly higher hepatic adenosine triphosphate content, cumulative bile production, and portal and arterial flows. Biliary secretion of bicarbonate, bilirubin, bile salts, and phospholipids was similar in all 3 groups. The alanine aminotransferase concentration in perfusate was lower in the HBOC\u2010201\u2013perfused groups. In conclusion, NMP of human donor livers can be performed effectively using HBOC\u2010201 and gelofusine, eliminating the need for human blood products. Perfusing livers with HBOC\u2010201 is at least similar to perfusion with RBCs and FFP. Some of the biomarkers of liver function and injury even suggest a possible superiority of an HBOC\u2010201\u2013based perfusion solution and opens a perspective for further optimization of machine perfusion techniques.<jats:italic toggle=\"yes\">Liver Transplantation 24 528\u2013538 2018<\/jats:italic>AASLD.<\/jats:p>","DOI":"10.1002\/lt.25005","type":"journal-article","created":{"date-parts":[[2017,12,27]],"date-time":"2017-12-27T23:02:41Z","timestamp":1514415761000},"page":"528-538","source":"Crossref","is-referenced-by-count":90,"title":["Normothermic machine perfusion of donor livers without the need for human blood products"],"prefix":"10.1097","volume":"24","author":[{"given":"Alix P. M.","family":"Matton","sequence":"first","affiliation":[{"name":"Surgical Research Laboratory,University of Groningen, University Medical Center Groningen,Groningen,the Netherlands"},{"name":"Section of Hepatobiliary Surgery and Liver Transplantation, Departments of Surgery,University of Groningen, University Medical Center Groningen,Groningen,the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura C.","family":"Burlage","sequence":"additional","affiliation":[{"name":"Surgical Research Laboratory,University of Groningen, University Medical Center Groningen,Groningen,the Netherlands"},{"name":"Section of Hepatobiliary Surgery and Liver Transplantation, Departments of Surgery,University of Groningen, University Medical Center Groningen,Groningen,the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rianne","family":"van Rijn","sequence":"additional","affiliation":[{"name":"Surgical Research Laboratory,University of Groningen, University Medical Center Groningen,Groningen,the Netherlands"},{"name":"Section of Hepatobiliary Surgery and Liver Transplantation, Departments of Surgery,University of Groningen, University Medical Center Groningen,Groningen,the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yvonne","family":"de Vries","sequence":"additional","affiliation":[{"name":"Surgical Research Laboratory,University of Groningen, University Medical Center Groningen,Groningen,the Netherlands"},{"name":"Section of Hepatobiliary Surgery and Liver Transplantation, Departments of Surgery,University of Groningen, University Medical Center Groningen,Groningen,the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shanice A.","family":"Karangwa","sequence":"additional","affiliation":[{"name":"Surgical Research Laboratory,University of Groningen, University Medical Center Groningen,Groningen,the Netherlands"},{"name":"Section of Hepatobiliary Surgery and Liver Transplantation, Departments of Surgery,University of Groningen, University Medical Center Groningen,Groningen,the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maarten W.","family":"Nijsten","sequence":"additional","affiliation":[{"name":"Department of Critical Care,University of Groningen, University Medical Center Groningen,Groningen,the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Annette S. H.","family":"Gouw","sequence":"additional","affiliation":[{"name":"Department of Pathology, University of Groningen,University Medical Center Groningen,Groningen,the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Janneke","family":"Wiersema\u2010Buist","sequence":"additional","affiliation":[{"name":"Surgical Research Laboratory,University of Groningen, University Medical Center Groningen,Groningen,the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jelle","family":"Adelmeijer","sequence":"additional","affiliation":[{"name":"Surgical Research Laboratory,University of Groningen, University Medical Center Groningen,Groningen,the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrie C.","family":"Westerkamp","sequence":"additional","affiliation":[{"name":"Surgical Research Laboratory,University of Groningen, University Medical Center Groningen,Groningen,the Netherlands"},{"name":"Section of Hepatobiliary Surgery and Liver Transplantation, Departments of Surgery,University of Groningen, University Medical Center Groningen,Groningen,the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ton","family":"Lisman","sequence":"additional","affiliation":[{"name":"Surgical Research Laboratory,University of Groningen, University Medical Center Groningen,Groningen,the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert J.","family":"Porte","sequence":"additional","affiliation":[{"name":"Section of Hepatobiliary Surgery and Liver Transplantation, Departments of Surgery,University of Groningen, University Medical Center Groningen,Groningen,the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","published-online":{"date-parts":[[2018,3,26]]},"reference":[{"key":"lt25005-bib-0001-20240115","doi-asserted-by":"crossref","first-page":"1265","DOI":"10.1111\/j.1600-6143.2007.01758.x","article-title":"Waitlist mortality decreases with increased use of extended criteria donor liver grafts at adult liver transplant centers","volume":"7","year":"2007","journal-title":"Am J Transplant"},{"issue":"suppl 2","key":"lt25005-bib-0002-20240115","first-page":"S5","article-title":"Opportunities for scientific expansion of the deceased donor pool","volume":"20","year":"2014","journal-title":"Liver Transpl"},{"key":"lt25005-bib-0003-20240115","doi-asserted-by":"crossref","first-page":"1779","DOI":"10.1111\/ajt.13708","article-title":"Liver transplantation after ex vivo normothermic machine preservation: a phase 1 (first\u2010in\u2010man) clinical trial","volume":"16","year":"2016","journal-title":"Am J Transplant"},{"key":"lt25005-bib-0004-20240115","doi-asserted-by":"crossref","first-page":"e110642","DOI":"10.1371\/journal.pone.0110642","article-title":"Criteria for viability assessment of discarded human donor livers during ex vivo normothermic machine perfusion","volume":"9","year":"2014","journal-title":"PLoS One"},{"key":"lt25005-bib-0005-20240115","doi-asserted-by":"crossref","first-page":"353","DOI":"10.1111\/ajt.13448","article-title":"Preimplant normothermic liver perfusion of a suboptimal liver donated after circulatory death","volume":"16","year":"2016","journal-title":"Am J Transplant"},{"key":"lt25005-bib-0006-20240115","doi-asserted-by":"crossref","first-page":"3235","DOI":"10.1111\/ajt.13875","article-title":"Transplantation of declined liver allografts following normothermic ex\u2010situ evaluation","volume":"16","year":"2016","journal-title":"Am J Transplant"},{"key":"lt25005-bib-0007-20240115","doi-asserted-by":"crossref","first-page":"1327","DOI":"10.1111\/ajt.12187","article-title":"Ex vivo normothermic machine perfusion and viability testing of discarded human donor livers","volume":"13","year":"2013","journal-title":"Am J Transplant"},{"key":"lt25005-bib-0008-20240115","doi-asserted-by":"crossref","first-page":"1071","DOI":"10.1111\/ajt.14049","article-title":"Preliminary single\u2010center canadian experience of human normothermic ex vivo liver perfusion: results of a clinical trial","volume":"17","year":"2017","journal-title":"Am J Transplant"},{"key":"lt25005-bib-0009-20240115","doi-asserted-by":"crossref","first-page":"1501","DOI":"10.1002\/lt.24499","article-title":"Normothermic ex vivo liver perfusion using steen solution as perfusate for human liver transplantation: First North American results","volume":"22","year":"2016","journal-title":"Liver Transpl"},{"key":"lt25005-bib-0010-20240115","doi-asserted-by":"crossref","first-page":"2746","DOI":"10.1097\/TP.0000000000001821","article-title":"The use of an acellular oxygen carrier in a human liver model of normothermic machine perfusion","volume":"101","year":"2017","journal-title":"Transplantation"},{"key":"lt25005-bib-0011-20240115","doi-asserted-by":"crossref","first-page":"825","DOI":"10.1097\/TP.0000000000001081","article-title":"Oxygenated hypothermic machine perfusion after static cold storage improves hepatobiliary function of extended criteria donor livers","volume":"100","year":"2016","journal-title":"Transplantation"},{"key":"lt25005-bib-0012-20240115","doi-asserted-by":"crossref","first-page":"161","DOI":"10.4244\/EIJV4I1A26","article-title":"HBOC\u2010201: the multi\u2010purpose oxygen therapeutic","volume":"4","year":"2008","journal-title":"Eurointervention"},{"issue":"suppl 1","key":"lt25005-bib-0013-20240115","first-page":"S76","article-title":"Hemoglobin substitutes","volume":"13","year":"2004","journal-title":"Eur Spine J"},{"key":"lt25005-bib-0014-20240115","doi-asserted-by":"crossref","first-page":"1331","DOI":"10.1093\/clinchem\/46.9.1331","article-title":"Mechanism of interference of a polymerized hemoglobin blood substitute in an alkaline phosphatase method","volume":"46","year":"2000","journal-title":"Clin Chem"},{"key":"lt25005-bib-0015-20240115","first-page":"e52688","article-title":"Ex situ normothermic machine perfusion of donor livers","volume":"99","year":"2015","journal-title":"J Vis Exp"},{"key":"lt25005-bib-0016-20240115","doi-asserted-by":"crossref","first-page":"924","DOI":"10.1016\/S0022-2275(20)40709-6","article-title":"Re\u2010evaluation of the 3 alpha\u2010hydroxysteroid dehydrogenase assay for total bile acids in bile","volume":"19","year":"1978","journal-title":"J Lipid Res"},{"key":"lt25005-bib-0017-20240115","doi-asserted-by":"crossref","first-page":"1265","DOI":"10.1097\/00007890-199306000-00011","article-title":"Neutrophil infiltration as an important factor in liver ischemia and reperfusion injury. Modulating effects of FK506 and cyclosporine","volume":"55","year":"1993","journal-title":"Transplantation"},{"key":"lt25005-bib-0018-20240115","doi-asserted-by":"crossref","first-page":"907","DOI":"10.1002\/bjs.10515","article-title":"Dual hypothermic oxygenated machine perfusion in liver transplants donated after circulatory death","volume":"104","year":"2017","journal-title":"Br J Surg"},{"key":"lt25005-bib-0019-20240115","doi-asserted-by":"crossref","first-page":"765","DOI":"10.1016\/j.jhep.2013.11.023","article-title":"HOPE for human liver grafts obtained from donors after cardiac death","volume":"60","year":"2014","journal-title":"J Hepatol"},{"key":"lt25005-bib-0020-20240115","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1111\/ajt.12958","article-title":"Hypothermic machine preservation facilitates successful transplantation of \u201corphan\u201d extended criteria donor livers","volume":"15","year":"2015","journal-title":"Am J Transplant"},{"key":"lt25005-bib-0021-20240115","doi-asserted-by":"crossref","first-page":"968","DOI":"10.1097\/01.sla.0000247056.85590.6b","article-title":"Novel short\u2010term hypothermic oxygenated perfusion (HOPE) system prevents injury in rat liver graft from nonheart beating donor","volume":"244","year":"2006","journal-title":"Ann Surg"},{"key":"lt25005-bib-0022-20240115","doi-asserted-by":"crossref","first-page":"120","DOI":"10.1002\/lt.24369","article-title":"First human liver transplantation using a marginal allograft resuscitated by normothermic machine perfusion","volume":"22","year":"2016","journal-title":"Liver Transpl"},{"key":"lt25005-bib-0023-20240115","doi-asserted-by":"crossref","first-page":"1035","DOI":"10.1002\/j.2040-4603.2013.tb00519.x","article-title":"Bile formation and secretion","volume":"3","year":"2013","journal-title":"Compr Physiol"},{"key":"lt25005-bib-0024-20240115","doi-asserted-by":"crossref","first-page":"381","DOI":"10.1111\/ajt.12991","article-title":"Liver preservation with machine perfusion and a newly developed cell\u2010free oxygen carrier solution under subnormothermic conditions","volume":"15","year":"2015","journal-title":"Am J Transplant"},{"key":"lt25005-bib-0025-20240115","doi-asserted-by":"crossref","first-page":"2789","DOI":"10.1111\/j.1600-6143.2012.04195.x","article-title":"The Eurotransplant donor risk index in liver transplantation: ET\u2010DRI","volume":"12","year":"2012","journal-title":"Am J Transplant"}],"updated-by":[{"DOI":"10.1002\/lt.25199","type":"erratum","label":"Erratum","source":"publisher","updated":{"date-parts":[[2018,8,24]],"date-time":"2018-08-24T00:00:00Z","timestamp":1535068800000}}],"container-title":["Liver Transplantation"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Flt.25005","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.lww.com\/01445473-201804000-00012","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,6,29]],"date-time":"2025-06-29T08:07:32Z","timestamp":1751184452000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.lww.com\/01445473-201804000-00012"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,3,26]]},"references-count":25,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2018]]}},"URL":"https:\/\/doi.org\/10.1002\/lt.25005","relation":{},"ISSN":["1527-6465","1527-6473"],"issn-type":[{"value":"1527-6465","type":"print"},{"value":"1527-6473","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,3,26]]}}}