{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T19:10:15Z","timestamp":1792782615360,"version":"4.5.0"},"reference-count":39,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"2","license":[{"start":{"date-parts":[[2019,1,31]],"date-time":"2019-01-31T00:00:00Z","timestamp":1548892800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2019,1,31]],"date-time":"2019-01-31T00:00:00Z","timestamp":1548892800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"name":"Berlin Institute for Health and the Charit\u00e9 - Universit\u00e4tsmedizin Berlin","id":[{"id":"https:\/\/ror.org\/001w7jn25","id-type":"ROR","asserted-by":"crossref"}]},{"name":"Charit\u00e9 - Universit\u00e4tsmedizin Berlin Clinician Scientist Program","id":[{"id":"https:\/\/ror.org\/001w7jn25","id-type":"ROR","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100002839","name":"Charit\u00e9 - Universit\u00e4tsmedizin Berlin","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100002839","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Einstein Center for Regenerative Therapies","id":[{"id":"https:\/\/ror.org\/02tmb7y21","id-type":"ROR","asserted-by":"crossref"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Liver Transpl"],"published-print":{"date-parts":[[2019,2]]},"abstract":"<jats:p>Normothermic ex vivo liver machine perfusion might be a superior preservation strategy for liver grafts from extended criteria donors. However, standardized small animal models are not available for basic research on machine perfusion of liver grafts. A laboratory\u2010scaled perfusion system was developed consisting of a custom\u2010made perfusion chamber, a pressure\u2010controlled roller pump, and an oxygenator. Male Wistar rat livers were perfused via the portal vein for 6 hours using oxygenated culture medium supplemented with rat erythrocytes. A separate circuit was connected via a dialysis membrane to the main circuit for plasma volume expansion. Glycine was added to the flush solution, the perfusate, and the perfusion circuit. Portal pressure and transaminase release were stable over the perfusion period. Dialysis significantly decreased the potassium concentration of the perfusate and led to significantly higher bile and total urea production. Hematoxylin\u2010eosin staining and immunostaining for single\u2010stranded DNA and activated caspase 3 showed less sinusoidal dilatation and tissue damage in livers treated with dialysis and glycine. Although Kupffer cells were preserved, tumor necrosis factor \u03b1 messenger RNA levels were significantly decreased by both treatments. For proof of concept, the optimized perfusion protocol was tested with donation after circulatory death (DCD) grafts, resulting in significantly lower transaminase release into the perfusate and preserved liver architecture compared with baseline perfusion. In conclusion, our laboratory\u2010scaled normothermic portovenous ex vivo liver perfusion system enables rat liver preservation for 6 hours. Both dialysis and glycine treatment were shown to be synergistic for preservation of the integrity of normal and DCD liver grafts.<\/jats:p>","DOI":"10.1002\/lt.25360","type":"journal-article","created":{"date-parts":[[2018,10,20]],"date-time":"2018-10-20T09:48:06Z","timestamp":1540028886000},"page":"275-287","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":28,"title":["Improvement of Normothermic Ex Vivo Machine Perfusion of Rat Liver Grafts by Dialysis and Kupffer Cell Inhibition With Glycine"],"prefix":"10.1097","volume":"25","author":[{"given":"Joseph M. G. V.","family":"Gassner","sequence":"first","affiliation":[{"name":"Experimental Surgery, Department of Surgery,Campus Charit\u00e9 Mitte | Campus Virchow\u2010Klinikum"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maximilian","family":"N\u00f6sser","sequence":"additional","affiliation":[{"name":"Experimental Surgery, Department of Surgery,Campus Charit\u00e9 Mitte | Campus Virchow\u2010Klinikum"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1879-4788","authenticated-orcid":false,"given":"Simon","family":"Moosburner","sequence":"additional","affiliation":[{"name":"Experimental Surgery, Department of Surgery,Campus Charit\u00e9 Mitte | Campus Virchow\u2010Klinikum"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rosa","family":"Horner","sequence":"additional","affiliation":[{"name":"Experimental Surgery, Department of Surgery,Campus Charit\u00e9 Mitte | Campus Virchow\u2010Klinikum"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Tang","sequence":"additional","affiliation":[{"name":"Experimental Surgery, Department of Surgery,Campus Charit\u00e9 Mitte | Campus Virchow\u2010Klinikum"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lara","family":"Wegener","sequence":"additional","affiliation":[{"name":"Experimental Surgery, Department of Surgery,Campus Charit\u00e9 Mitte | Campus Virchow\u2010Klinikum"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Wyrwal","sequence":"additional","affiliation":[{"name":"Experimental Surgery, Department of Surgery,Campus Charit\u00e9 Mitte | Campus Virchow\u2010Klinikum"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Felix","family":"Claussen","sequence":"additional","affiliation":[{"name":"Experimental Surgery, Department of Surgery,Campus Charit\u00e9 Mitte | Campus Virchow\u2010Klinikum"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruza","family":"Arsenic","sequence":"additional","affiliation":[{"name":"Institute of Pathology,Charit\u00e9 \u2013 Universit\u00e4tsmedizin Berlin,Berlin,Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Johann","family":"Pratschke","sequence":"additional","affiliation":[{"name":"Experimental Surgery, Department of Surgery,Campus Charit\u00e9 Mitte | Campus Virchow\u2010Klinikum"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Igor M.","family":"Sauer","sequence":"additional","affiliation":[{"name":"Experimental Surgery, Department of Surgery,Campus Charit\u00e9 Mitte | Campus Virchow\u2010Klinikum"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nathanael","family":"Raschzok","sequence":"additional","affiliation":[{"name":"Experimental Surgery, Department of Surgery,Campus Charit\u00e9 Mitte | Campus Virchow\u2010Klinikum"},{"name":"Charit\u00e9 Clinician Scientist Program, Berlin Institute of Health,Berlin,Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","published-online":{"date-parts":[[2019,1,31]]},"reference":[{"key":"lt25360-bib-0001-20240115","doi-asserted-by":"crossref","first-page":"1245","DOI":"10.1016\/j.jhep.2016.07.033","article-title":"NAFLD and liver transplantation: current burden and expected challenges","volume":"65","year":"2016","journal-title":"J Hepatol"},{"key":"lt25360-bib-0002-20240115","doi-asserted-by":"crossref","first-page":"1136","DOI":"10.1002\/lt.24461","article-title":"Liver transplantation in Germany","volume":"22","year":"2016","journal-title":"Liver Transpl"},{"key":"lt25360-bib-0004-20240115","doi-asserted-by":"crossref","first-page":"4966","DOI":"10.3748\/wjg.v22.i21.4966","article-title":"How important is donor age in liver transplantation?","volume":"22","year":"2016","journal-title":"World J 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