{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,12]],"date-time":"2026-02-12T08:43:32Z","timestamp":1770885812125,"version":"3.50.1"},"reference-count":36,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"10","license":[{"start":{"date-parts":[[2019,9,10]],"date-time":"2019-09-10T00:00:00Z","timestamp":1568073600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2019,9,10]],"date-time":"2019-09-10T00:00:00Z","timestamp":1568073600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Liver Transpl"],"published-print":{"date-parts":[[2019,10]]},"abstract":"<jats:p>The human liver\u2019s capacity to rapidly regenerate to a full\u2010sized functional organ after resection has allowed successful outcomes for living donor liver transplantation (LDLT) procedures. However, the ability to detect and track physiological changes occurring during liver regeneration after resection and throughout the restoration process is still lacking. We performed a comprehensive whole\u2010transcriptome RNA sequencing analysis of liver and circulating blood tissue from 12 healthy LDLT donors to define biomarker signatures for monitoring physiological activities during liver regeneration at 14 time points for up to a 1\u2010year procedural follow\u2010up. LDLT donor liver tissue differentially expressed 1238 coding and noncoding genes after resection, and an additional 1260 genes were selectively regulated after LDLT. A total of 15,011 RNA transcript species were identified in the blood in response to liver resection. The transcripts most highly regulated were sequentially expressed within 3 distinct peaks that correlated with sets of functional genes involved in the induction of liver resection\u2013specific innate immune response (peak 1), activation of the complement system (peak 2), and platelet activation and erythropoiesis (peak 3). Each peak corresponded with progressive phases of extracellular matrix degradation, remodeling, and organization during liver restoration. These processes could be tracked by distinct molecular signatures of up\u2010regulated and down\u2010regulated gene profiles in the blood during phases of liver repair and regeneration. In conclusion, the results establish temporal and dynamic transcriptional patterns of gene expression following surgical liver resection that can be detected in the blood and potentially used as biomarker signatures for monitoring phases of liver regeneration.<\/jats:p>","DOI":"10.1002\/lt.25608","type":"journal-article","created":{"date-parts":[[2019,7,24]],"date-time":"2019-07-24T20:18:51Z","timestamp":1563999531000},"page":"1541-1560","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Profiling Gene Programs in the Blood During Liver Regeneration in Living Liver Donors"],"prefix":"10.1097","volume":"25","author":[{"given":"Michael C.","family":"Lawrence","sequence":"first","affiliation":[{"name":"Baylor Scott & White Research Institute,Dallas,TX"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carly M.","family":"Darden","sequence":"additional","affiliation":[{"name":"Institute of Biomedical Studies,Baylor University,Waco,TX"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Srividya","family":"Vasu","sequence":"additional","affiliation":[{"name":"Baylor Scott & White Research Institute,Dallas,TX"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kenjiro","family":"Kumano","sequence":"additional","affiliation":[{"name":"Baylor Scott & White Research Institute,Dallas,TX"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jinghua","family":"Gu","sequence":"additional","affiliation":[{"name":"Baylor Scott & White Research Institute,Dallas,TX"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xuan","family":"Wang","sequence":"additional","affiliation":[{"name":"Baylor Scott & White Research Institute,Dallas,TX"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jinyan","family":"Chan","sequence":"additional","affiliation":[{"name":"Baylor Scott & White Research Institute,Dallas,TX"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhaohui","family":"Xu","sequence":"additional","affiliation":[{"name":"Baylor Scott & White Research Institute,Dallas,TX"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Benjamin F.","family":"Lemoine","sequence":"additional","affiliation":[{"name":"Baylor Scott & White Research Institute,Dallas,TX"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Phuong","family":"Nguyen","sequence":"additional","affiliation":[{"name":"Baylor Scott & White Research Institute,Dallas,TX"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cynthia","family":"Smitherman","sequence":"additional","affiliation":[{"name":"Baylor Scott & White Research Institute,Dallas,TX"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bashoo","family":"Naziruddin","sequence":"additional","affiliation":[{"name":"Annette C. and Harold C. 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