{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T04:48:48Z","timestamp":1790138928903,"version":"4.0.1"},"reference-count":30,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"1","license":[{"start":{"date-parts":[[2019,12,26]],"date-time":"2019-12-26T00:00:00Z","timestamp":1577318400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2019,12,26]],"date-time":"2019-12-26T00:00:00Z","timestamp":1577318400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100000062","name":"National Institute of Diabetes and Digestive and Kidney Diseases","doi-asserted-by":"publisher","award":["DK106667"],"award-info":[{"award-number":["DK106667"]}],"id":[{"id":"10.13039\/100000062","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000062","name":"National Institute of Diabetes and Digestive and Kidney Diseases","doi-asserted-by":"publisher","award":["DK113339"],"award-info":[{"award-number":["DK113339"]}],"id":[{"id":"10.13039\/100000062","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000062","name":"National Institute of Diabetes and Digestive and Kidney Diseases","doi-asserted-by":"publisher","award":["DK117861"],"award-info":[{"award-number":["DK117861"]}],"id":[{"id":"10.13039\/100000062","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Liver Transpl"],"published-print":{"date-parts":[[2020,1]]},"abstract":"<jats:p>Biliary complications (strictures and leaks) represent major limitations in living donor liver transplantation. Mesenchymal stem cells (MSCs) are a promising modality to prevent biliary complications because of immunosuppressive and angiogenic properties. Our goal was to evaluate the safety of adipose\u2010derived MSC delivery to biliary anastomoses in a porcine model. Secondary objectives were defining the optimal method of delivery (intraluminal versus extraluminal) and to investigate MSC engraftment, angiogenesis, and fibrosis. Pigs were divided into 3 groups. Animals underwent adipose collection, MSC isolation, and expansion. Two weeks later, animals underwent bile duct transection, reanastomosis, and stent insertion. Group 1 received plastic stents wrapped in unseeded Vicryl mesh. Group 2 received stents wrapped in MSC\u2010seeded mesh. Group 3 received unwrapped stents with the anastomosis immersed in an MSC suspension. Animals were killed 1 month after stent insertion when cholangiograms and biliary tissue were obtained. Serum was collected for liver biochemistries. Tissue was used for hematoxylin\u2010eosin and trichrome staining and immunohistochemistry for MSC markers (CD44 and CD34) and for a marker of neoangiogenesis (CD31). There were no intraoperative complications. One pig died on postoperative day 3 due to acute cholangitis. All others recovered without complications. Cholangiography demonstrated no biliary leaks and minimal luminal narrowing. Surviving animals exhibited no symptoms, abnormal liver biochemistries, or clinically significant biliary stricturing. Group 3 showed significantly greater CD44 and CD34 staining, indicating MSC engraftment. Fibrosis was reduced at the anastomotic site in group 3 based on trichrome stain. CD31 staining of group 3 was more pronounced, supporting enhanced neoangiogenesis. In conclusion, adipose\u2010derived MSCs were safely applied to biliary anastomoses. MSCs were locally engrafted within the bile duct and may have beneficial effects in terms of fibrosis and angiogenesis.<\/jats:p>","DOI":"10.1002\/lt.25682","type":"journal-article","created":{"date-parts":[[2019,11,19]],"date-time":"2019-11-19T18:45:38Z","timestamp":1574189138000},"page":"100-112","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Autologous Adipose Tissue\u2013Derived Mesenchymal Stem Cells Introduced by Biliary Stents or Local Immersion in Porcine Bile Duct Anastomoses"],"prefix":"10.1097","volume":"26","author":[{"given":"Yi","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Surgery,Mayo Clinic and Foundation,Rochester,MN"},{"name":"Chongqing University Cancer Hospital,Chongqing,China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ayushman","family":"Sharma","sequence":"additional","affiliation":[{"name":"Department of Surgery,Mayo Clinic and Foundation,Rochester,MN"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dong Jin","family":"Joo","sequence":"additional","affiliation":[{"name":"Department of Surgery,Mayo Clinic and Foundation,Rochester,MN"},{"name":"Department of Surgery,Yonsei University,Seoul,Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erek","family":"Nelson","sequence":"additional","affiliation":[{"name":"Department of Surgery,Mayo Clinic and Foundation,Rochester,MN"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6743-8280","authenticated-orcid":false,"given":"Anan","family":"AbuRmilah","sequence":"additional","affiliation":[{"name":"Department of Surgery,Mayo Clinic and Foundation,Rochester,MN"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bruce P.","family":"Amiot","sequence":"additional","affiliation":[{"name":"Department of Surgery,Mayo Clinic and Foundation,Rochester,MN"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christen J.","family":"Boyer","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cellular Physiology,Louisiana State University Health Sciences Center,Shreveport,LA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jonathan S.","family":"Alexander","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cellular Physiology,Louisiana State University Health Sciences Center,Shreveport,LA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nidhi","family":"Jalan\u2010Sakrikar","sequence":"additional","affiliation":[{"name":"Gastroenterology Research Unit,Mayo Clinic and Foundation,Rochester,MN"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John","family":"Martin","sequence":"additional","affiliation":[{"name":"Division of Gastroenterology and Hepatology,Mayo Clinic and Foundation,Rochester,MN"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roger","family":"Moreira","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine and Pathology,Mayo Clinic and Foundation,Rochester,MN"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shiraj A.","family":"Chowdhury","sequence":"additional","affiliation":[{"name":"Gastroenterology Research Unit,Mayo Clinic and Foundation,Rochester,MN"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michele","family":"Smart","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine and Pathology,Mayo Clinic and Foundation,Rochester,MN"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Allan B.","family":"Dietz","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine and Pathology,Mayo Clinic and Foundation,Rochester,MN"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Scott L.","family":"Nyberg","sequence":"additional","affiliation":[{"name":"Department of Surgery,Mayo Clinic and Foundation,Rochester,MN"},{"name":"William J. von Liebig Center for Transplantation and Clinical Regeneration,Mayo Clinic and Foundation,Rochester,MN"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julie K.","family":"Heimbach","sequence":"additional","affiliation":[{"name":"Department of Surgery,Mayo Clinic and Foundation,Rochester,MN"},{"name":"William J. von Liebig Center for Transplantation and Clinical Regeneration,Mayo Clinic and Foundation,Rochester,MN"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9812-7573","authenticated-orcid":false,"given":"Robert C.","family":"Huebert","sequence":"additional","affiliation":[{"name":"Gastroenterology Research Unit,Mayo Clinic and Foundation,Rochester,MN"},{"name":"Division of Gastroenterology and Hepatology,Mayo Clinic and Foundation,Rochester,MN"},{"name":"William J. von Liebig Center for Transplantation and Clinical Regeneration,Mayo Clinic and Foundation,Rochester,MN"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","published-online":{"date-parts":[[2019,12,26]]},"reference":[{"key":"lt25682-bib-0001-20240115","doi-asserted-by":"crossref","first-page":"1026","DOI":"10.1016\/S0016-5085(73)80017-4","article-title":"Liver transplantation in man: the frequency of rejection, biliary tract complications, and recurrence of malignancy based on an analysis of 26 cases","volume":"64","year":"1973","journal-title":"Gastroenterology"},{"key":"lt25682-bib-0002-20240115","doi-asserted-by":"crossref","first-page":"430","DOI":"10.1097\/00000658-200009000-00015","article-title":"Postoperative bile duct strictures: management and outcome in the 1990s","volume":"232","year":"2000","journal-title":"Ann 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transplantation: feasibility of duct\u2010to\u2010duct reconstruction with a T\u2010tube stent","volume":"41","year":"2009","journal-title":"Transplant Proc"},{"key":"lt25682-bib-0012-20240115","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1111\/ans.13827","article-title":"Systematic review and meta\u2010analysis of studies of biliary reconstruction in adult living donor liver transplantation","volume":"87","year":"2017","journal-title":"ANZ J Surg"},{"key":"lt25682-bib-0013-20240115","doi-asserted-by":"crossref","first-page":"850","DOI":"10.1016\/j.transproceed.2013.11.035","article-title":"Biliary complications in 108 consecutive recipients with duct\u2010to\u2010duct biliary reconstruction in living\u2010donor liver transplantation","volume":"46","year":"2014","journal-title":"Transplant Proc"},{"key":"lt25682-bib-0014-20240115","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1007\/s12328-017-0754-z","article-title":"Endoscopic management of biliary strictures 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VEGF\u2010dependent angiogenesis","volume":"33","year":"2012","journal-title":"Biomaterials"},{"key":"lt25682-bib-0021-20240115","first-page":"1115","article-title":"Mesenchymal stem cells contribute to improvement of renal function in a canine kidney injury model","volume":"31","year":"2017","journal-title":"In Vivo"},{"key":"lt25682-bib-0022-20240115","doi-asserted-by":"crossref","first-page":"1281","DOI":"10.1016\/S0140-6736(16)31203-X","article-title":"Expanded allogeneic adipose\u2010derived mesenchymal stem cells (Cx601) for complex perianal fistulas in Crohn's disease: a phase 3 randomised, double\u2010blind controlled trial","volume":"388","year":"2016","journal-title":"Lancet"},{"key":"lt25682-bib-0023-20240115","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1053\/j.gastro.2017.04.001","article-title":"Autologous mesenchymal stem cells, applied in a bioabsorbable matrix, for treatment of perianal fistulas in patients with Crohn\u2019s 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lesions using a scaffold\u2010free tissue\u2010engineered construct derived from allogenic synovial MSCs in a miniature swine model","volume":"34","year":"2013","journal-title":"Biomaterials"}],"container-title":["Liver 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History"}},{"value":"2019-10-29","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-12-26","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}