{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,8]],"date-time":"2026-06-08T05:12:02Z","timestamp":1780895522750,"version":"3.54.1"},"reference-count":31,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"5","license":[{"start":{"date-parts":[[2021,4,30]],"date-time":"2021-04-30T00:00:00Z","timestamp":1619740800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2021,4,30]],"date-time":"2021-04-30T00:00:00Z","timestamp":1619740800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":["Liver Transpl."],"published-print":{"date-parts":[[2021,5]]},"abstract":"<jats:p>Living donor liver transplantation (LDLT) is sometimes associated with impaired regeneration and severe ischemia\/reperfusion injury (IRI) in the graft, resulting in small\u2010for\u2010size syndrome (SFSS). Platelets were previously reported to stimulate liver regeneration in models of hepatectomy, but the evidence in partial liver transplantation (LT) is lacking. In this study, a rat model of partial LT was used, and the impact of thrombopoietin (TPO)\u2010induced perioperative thrombocytosis on graft regeneration, IRI, and survival was investigated. In experiment 1, a 30% partial LT was performed. Under thrombocytosis, SFSS was attenuated, as shown by decreased levels of serum aminotransferases, bilirubin, and ascites. Serum hepatocyte regeneration\u2013related cytokines, including insulin\u2010like growth factor\u20101, hepatocyte growth factor, interleukin 6 (IL6), and tumor necrosis factor \u03b1 (TNF\u2010\u03b1), were elevated. In addition, the proliferative signaling pathways, Ki\u201067\u2010labeling index, proliferating cell nuclear antigen (PCNA)\u2013labeling index, mitotic index, and liver\/body weight ratio were increased under thrombocytosis. The platelet\u2010induced regeneration was independent of TPO because increases in the Ki\u201067\u2010labeling and PCNA\u2010labeling indexes were eliminated after reducing platelet counts by antiplatelet serum in rats administered with TPO. For IRI, thrombocytosis did not aggravate oxidative stress or downstream signaling pathways, necrosis, or apoptosis in the graft. After Kupffer cell (KC) depletion, the platelet\u2010induced attenuation of serum aminotransferases, increased serum levels of IL6 and TNF\u2010\u03b1, and proliferation\u2010related signaling pathways were eliminated. Moreover, platelet accumulation in the graft decreased substantially. In experiment 2, a 20% partial LT was performed, and thrombocytosis improved postoperative survival. In conclusion, our results suggested that thrombocytosis stimulated graft regeneration and prolonged survival without aggregating IRI after partial LT, and KCs vitally contributed to platelet\u2010derived regeneration. Platelet therapies to increase perioperative platelet counts may improve the outcomes after LDLT.<\/jats:p>","DOI":"10.1002\/lt.25962","type":"journal-article","created":{"date-parts":[[2020,12,4]],"date-time":"2020-12-04T22:45:06Z","timestamp":1607121906000},"page":"719-734","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":17,"title":["Platelets Stimulate Liver Regeneration in a Rat Model of Partial Liver Transplantation"],"prefix":"10.1097","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4528-7303","authenticated-orcid":false,"given":"Chen","family":"Liang","sequence":"first","affiliation":[{"name":"Department of Gastrointestinal and Hepatobiliary\u2010Pancreatic Surgery,Graduate School of Medicine,University of Tsukuba,Tsukuba,Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1089-0644","authenticated-orcid":false,"given":"Kazuhiro","family":"Takahashi","sequence":"additional","affiliation":[{"name":"Department of Gastrointestinal and Hepatobiliary\u2010Pancreatic Surgery,Graduate School of Medicine,University of Tsukuba,Tsukuba,Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2630-3072","authenticated-orcid":false,"given":"Kinji","family":"Furuya","sequence":"additional","affiliation":[{"name":"Department of Gastrointestinal and Hepatobiliary\u2010Pancreatic Surgery,Graduate School of Medicine,University of Tsukuba,Tsukuba,Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6115-0158","authenticated-orcid":false,"given":"Tatsuya","family":"Oda","sequence":"additional","affiliation":[{"name":"Department of Gastrointestinal and 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