{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T01:45:07Z","timestamp":1790387107172,"version":"4.1.0"},"reference-count":42,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2006,7,6]],"date-time":"2006-07-06T00:00:00Z","timestamp":1152144000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Macromolecular Bioscience"],"published-print":{"date-parts":[[2006,7,14]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:bold>Summary:<\/jats:bold> In order to reveal mechanisms for the electrospinning of proteins, this study focuses on the polymer chain conformation, which is considered to be a critical factor for successful electrospinning. Poly(<jats:italic>\u03b3<\/jats:italic>\u2010benzyl\u2010<jats:sc>L<\/jats:sc>\u2010glutamate) (PBLG) is employed and the relationships between the chain conformations of the pre\u2010spun PBLG molecules and the morphologies of the post\u2010spun PBLG fibers are investigated. By combining viscosity measurements, and circular dichroism and FT\u2010IR spectroscopies, chain conformations of the pre\u2010 and post\u2010spun PBLG are characterized. The chain conformations of the pre\u2010spun PBLG changes from an <jats:italic>\u03b1<\/jats:italic>\u2010helix to a random coil upon changing the solvent ratios of dichloromethane (CH<jats:sub>2<\/jats:sub>Cl<jats:sub>2<\/jats:sub>) and trifluoroacetic acid (CF<jats:sub>3<\/jats:sub>COOH) from 100:0 to 0:100. In an <jats:italic>\u03b1<\/jats:italic>\u2010helix conformation, the morphology of PBLG fibers is relatively thick, while that of the random coil is thin and homogenous. The mean fiber diameters decrease when the chain conformations change from an <jats:italic>\u03b1<\/jats:italic>\u2010helix to a random coil. FT\u2010IR spectroscopy and wide\u2010angle X\u2010ray diffraction measurements reveal that electrospinning predominantly induces an <jats:italic>\u03b1<\/jats:italic>\u2010helical conformation in post\u2010spun PBLG fibers, and more highly crystallized fibers are generated as the <jats:italic>\u03b1<\/jats:italic>\u2010helical content in the pre\u2010spun solution increases.<\/jats:p><jats:p><jats:boxed-text content-type=\"graphic\" position=\"anchor\"><jats:caption><jats:p>SEM images of the electrospun PBLG fibers (<jats:styled-content>$\\overline {DP}$<jats:inline-graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"graphic\/tex2gif-ueqn-1.gif\" xlink:title=\"equation image\"\/><\/jats:styled-content>\u2009=\u20091\u2009200) at DCM\/TFA ratios of a) 100:0, b) 90:10, c) 80:20, and d) 0:100 (magnification: \u00d71\u2009000, scale bars: 20 \u00b5m).<\/jats:p><\/jats:caption><jats:graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" mimetype=\"image\/jpeg\" position=\"anchor\" specific-use=\"enlarged-web-image\" xlink:href=\"graphic\/mgra001.jpg\"><jats:alt-text>magnified image<\/jats:alt-text><jats:caption><jats:p>SEM images of the electrospun PBLG fibers (<jats:styled-content>$\\overline {DP}$<jats:inline-graphic xlink:href=\"graphic\/tex2gif-ueqn-1.gif\" xlink:title=\"equation image\"\/><\/jats:styled-content>\u2009=\u20091\u2009200) at DCM\/TFA ratios of a) 100:0, b) 90:10, c) 80:20, and d) 0:100 (magnification: \u00d71\u2009000, scale bars: 20 \u00b5m).<\/jats:p><\/jats:caption><\/jats:graphic><\/jats:boxed-text>\n<\/jats:p>","DOI":"10.1002\/mabi.200600066","type":"journal-article","created":{"date-parts":[[2006,7,10]],"date-time":"2006-07-10T18:08:52Z","timestamp":1152554932000},"page":"487-495","source":"Crossref","is-referenced-by-count":30,"title":["Chain Conformations of Poly(<i>\u03b3<\/i>\u2010benzyl\u2010<scp>L<\/scp>\u2010glutamate) Pre and Post an Electrospinning Process"],"prefix":"10.1002","volume":"6","author":[{"given":"Ken\u2010Ichi","family":"Minato","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kousaku","family":"Ohkawa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroyuki","family":"Yamamoto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2006,7,6]]},"reference":[{"key":"e_1_2_6_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/0304-3886(95)00041-8"},{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0266-3538(03)00178-7"},{"key":"e_1_2_6_3_2","first-page":"727","volume":"6","author":"Lyons J.","year":"2004","journal-title":"Encycl. 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