{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T09:17:30Z","timestamp":1777454250649,"version":"3.51.4"},"reference-count":32,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2006,9,11]],"date-time":"2006-09-11T00:00:00Z","timestamp":1157932800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Macromolecular Bioscience"],"published-print":{"date-parts":[[2006,9,15]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:bold>Summary:<\/jats:bold> A series of terpolymers made of DMA, NAS and MAPS were synthesized by free radical copolymerization and used as functional coatings for the fabrication of glass slide DNA microarrays. The surface properties of coated glass slides were investigated through contact angle measurements, ellipsometry and atomic force microscopy. The terpolymer molecular weight showed a moderate effect on surface tension (<jats:italic>\u03b3<\/jats:italic><jats:sub>s<\/jats:sub>\u2009=\u200956\u201362 mN\u2009\u00b7\u2009m<jats:sup>\u22121<\/jats:sup>), but no clear effect on polymeric layer thickness (5\u20138 nm) and roughness. Hybridization experiments with amine\u2010functionalized oligonucleotides gave the best fluorescence intensity results for microarrays coated with intermediate\u2010molecular\u2010weight terpolymers. Finally, an accelerated ageing test of the microarray in a humidity chamber showed a nice relationship between decay curves of contact angle against water and fluorescence intensity.<\/jats:p><jats:p><jats:boxed-text content-type=\"graphic\" position=\"anchor\"><jats:caption><jats:p>Details of fluorescence microscope images after hybridization of the probe H<jats:sub>2<\/jats:sub>N\u2010oligonucleotide spotted on the polymeric coatings.<\/jats:p><\/jats:caption><jats:graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" mimetype=\"image\/jpeg\" position=\"anchor\" specific-use=\"enlarged-web-image\" xlink:href=\"graphic\/mgra001.jpg\"><jats:alt-text>magnified image<\/jats:alt-text><jats:caption><jats:p>Details of fluorescence microscope images after hybridization of the probe H<jats:sub>2<\/jats:sub>N\u2010oligonucleotide spotted on the polymeric coatings.<\/jats:p><\/jats:caption><\/jats:graphic><\/jats:boxed-text>\n<\/jats:p>","DOI":"10.1002\/mabi.200600088","type":"journal-article","created":{"date-parts":[[2006,9,11]],"date-time":"2006-09-11T18:34:33Z","timestamp":1157999673000},"page":"719-729","source":"Crossref","is-referenced-by-count":17,"title":["Surface Behavior and Molecular Recognition in DNA Microarrays from <i>N<\/i>,<i>N<\/i>\u2010Dimethylacrylamide Terpolymers with Activated Esters as Linking Groups"],"prefix":"10.1002","volume":"6","author":[{"given":"Raffaella","family":"Suriano","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marinella","family":"Levi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Giovanna","family":"Pirri","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Francesco","family":"Damin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marcella","family":"Chiari","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stefano","family":"Turri","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,9,28]]},"reference":[{"key":"e_1_2_6_1_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.270.5235.467"},{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jviromet.2004.02.018"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jviromet.2004.06.016"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/ng1296-457"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.94.6.2150"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.91.11.5022"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/86730"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1002\/1521-3773(20020415)41:8<1276::AID-ANIE1276>3.0.CO;2-2"},{"key":"e_1_2_6_9_2","first-page":"76","author":"Luhman W.","year":"2005","journal-title":"NNIN REU Research Accomplishments"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-9936(04)00104-9"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1006\/abio.1997.2017"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/27.9.1970"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gng088"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1021\/la046951y"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/24.16.3142"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1006\/abio.1998.2620"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/27.2.649"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1186\/1472-6750-3-6"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1021\/bc960046a"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1021\/la00022a042"},{"key":"e_1_2_6_21_2","doi-asserted-by":"publisher","DOI":"10.1021\/bc049791a"},{"key":"e_1_2_6_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/0966-7822(96)00001-9"},{"key":"e_1_2_6_23_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac0352629"},{"key":"e_1_2_6_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ab.2004.05.041"},{"key":"e_1_2_6_25_2","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200401216"},{"key":"e_1_2_6_26_2","first-page":"39","volume-title":"Polymer Surface Modification and Characterization","author":"Chan C. 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