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In this study, we covalently bound fibronectin (FN) onto ammonia plasma\u2010treated PTFE via two crosslinkers, namely glutaric anhydride (GA) and sulfosuccinimidyl\u20104\u2010(<jats:italic>p<\/jats:italic>\u2010maleimidophenyl)butyrate (sulfo\u2010SMPB). With respect to clean PTFE, cell adhesion increased markedly on both FN grafted surfaces, although it was twice higher on PTFE\u2010GA\u2010FN than on PTFE\u2010SMPB\u2010FN. ELISA experiments performed with a polyclonal antibody revealed that the amount of FN is identical on both surfaces while monoclonal antibody specific to the RGD binding site clearly demonstrated a greater availability when FN is surface grafted through GA. These results provide evidence of a variation in protein conformation correlated with the surface conjugation strategy.<\/jats:p><jats:p><jats:boxed-text content-type=\"graphic\" position=\"anchor\"><jats:graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" mimetype=\"image\/jpeg\" position=\"anchor\" specific-use=\"enlarged-web-image\" xlink:href=\"graphic\/mgra001.jpg\"><jats:alt-text>magnified image<\/jats:alt-text><\/jats:graphic><\/jats:boxed-text>\n<\/jats:p>","DOI":"10.1002\/mabi.200600267","type":"journal-article","created":{"date-parts":[[2007,4,25]],"date-time":"2007-04-25T07:47:31Z","timestamp":1177487251000},"page":"738-745","source":"Crossref","is-referenced-by-count":51,"title":["Covalent Grafting of Fibronectin onto Plasma\u2010Treated PTFE: Influence of the Conjugation Strategy on Fibronectin Biological 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