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Since commonly used heat and ionizing energy\u2010based sterilization methods have been shown to alter the material properties of protein\u2010based scaffolds, the effects of ethanol and ethylene oxide (EtO) sterilization on the cellular compatibility and the structural, chemical, and mechanical properties of uncrosslinked, UV crosslinked, or 1\u2010ethyl\u20103\u2010(3\u2010dimethylaminopropyl)carbodiimide (EDC) crosslinked fibrin microthreads in neutral (EDCn) or acidic (EDCa) buffers are evaluated. EtO sterilization significantly reduces the tensile strength of uncrosslinked microthreads. Surface chemistry analyses show that EtO sterilization induces alkylation of EDCa microthreads leading to a significant reduction in myoblast attachment. The material properties of EDCn microthreads do not appear to be affected by the sterilization method. These results significantly enhance the understanding of how sterilization or crosslinking techniques affect the material properties of protein scaffolds.\n<jats:boxed-text content-type=\"graphic\" position=\"anchor\"><jats:graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" mimetype=\"image\/png\" position=\"anchor\" specific-use=\"enlarged-web-image\" xlink:href=\"graphic\/mabi201500410-abs-0001-m.png\"><jats:alt-text>image<\/jats:alt-text><\/jats:graphic><\/jats:boxed-text><\/jats:p>","DOI":"10.1002\/mabi.201500410","type":"journal-article","created":{"date-parts":[[2016,2,5]],"date-time":"2016-02-05T04:51:37Z","timestamp":1454647897000},"page":"836-846","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["The Effect of Sterilization Methods on the Structural and Chemical Properties of Fibrin Microthread Scaffolds"],"prefix":"10.1002","volume":"16","author":[{"given":"Jonathan M.","family":"Grasman","sequence":"first","affiliation":[{"name":"Department of Biomedical Engineering Worcester Polytechnic Institute  Worcester MA 01609 USA"},{"name":"Bioengineering Institute Worcester Polytechnic Institute  Worcester MA 01609 USA"},{"name":"Department of Biomedical Engineering Tufts University  Medford MA 02155 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Megan P.","family":"O'Brien","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering Worcester Polytechnic Institute  Worcester MA 01609 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kevin","family":"Ackerman","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering Worcester Polytechnic Institute  Worcester MA 01609 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keith A.","family":"Gagnon","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering Worcester Polytechnic Institute  Worcester MA 01609 USA"},{"name":"Boston University School of Medicine  Boston MA 02119 USA"},{"name":"Department of Biomedical Engineering Boston University  Boston MA 02215 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gregory M.","family":"Wong","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering Worcester Polytechnic Institute  Worcester MA 01609 USA"},{"name":"Department of Geosciences Pennsylvania State University  University Park PA 16802 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"George D.","family":"Pins","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering Worcester Polytechnic Institute  Worcester MA 01609 USA"},{"name":"Bioengineering Institute Worcester Polytechnic Institute  Worcester MA 01609 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,2,4]]},"reference":[{"key":"e_1_2_6_1_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.b.33070"},{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.b.30885"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.b.32928"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.b.33069"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1586\/17434440.2014.900436"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajic.2006.10.014"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0142-9612(00)00136-8"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/0196-6553(94)90004-3"},{"key":"e_1_2_6_9_1","volume-title":"Biomaterials Science: An Introduction to Materials in Medicine","author":"Ratner B. 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