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High molecular weight fractions of these polymers are collected by ultrafiltration and characterized by chemical analysis,<jats:sup>1<\/jats:sup>H and<jats:sup>13<\/jats:sup>C\u2010NMR spectroscopy, size\u2010exclusion chromatography with triple detection, and potentiometric titration, and are tested for DNA delivery in vitro. It is shown that PHPI\u2010CA are highly branched polymers containing disulfide linkages. Transfection efficiency of PHPI\u2010CA for DNA gives similar results to that of PHPI with GFP<jats:sup>+<\/jats:sup>cell percent reaching 80\u201390%. Cytotoxicity levels for PHPI\u2010CA are lower than that of PHPI. Novel polymers containing different amounts of disulfide linkages are able to disintegrate and release DNA following the treatment with reducing agent 1,4\u2010dithiothreitol. 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