{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,7]],"date-time":"2025-10-07T04:52:19Z","timestamp":1759812739673,"version":"3.41.2"},"reference-count":58,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2016,9,28]],"date-time":"2016-09-28T00:00:00Z","timestamp":1475020800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2016,9,28]],"date-time":"2016-09-28T00:00:00Z","timestamp":1475020800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1"},{"start":{"date-parts":[[2016,9,28]],"date-time":"2016-09-28T00:00:00Z","timestamp":1475020800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions"}],"funder":[{"DOI":"10.13039\/501100001868","name":"National Science Council","doi-asserted-by":"publisher","award":["103\u20102221\u2010E\u2010010\u2010006"],"award-info":[{"award-number":["103\u20102221\u2010E\u2010010\u2010006"]}],"id":[{"id":"10.13039\/501100001868","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001868","name":"National Science Council","doi-asserted-by":"publisher","award":["103-2221-E-010-006"],"award-info":[{"award-number":["103-2221-E-010-006"]}],"id":[{"id":"10.13039\/501100001868","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["wiley.com"],"crossmark-restriction":true},"short-container-title":["Macromolecular Bioscience"],"published-print":{"date-parts":[[2017,3]]},"abstract":"<jats:p>Bone marrow\u2010derived mesenchymal stem cell microtissues (BMSCMT) enhanced cardiomyogenesis in vitro and cardiac repairs of myocardial infarcted hearts in vivo are documented. Producing human BMSCMT onto patches in vitro for cardiac tissue engineering has not been reported. For possibly producing human bone marrow\u2013derived mesenchymal stem cell microtissues (hBMSCMT) on an elastic silk fibroin (SF)\u2010poly(\u03b5\u2010caprolactone) (PCL) based patches is hereby designed. After an elastic SF\u2010PCL (SP) patch is fabricated, hyaluronic acid (HA)\/SF\u2010PCL(HSP) and HA\u2010GRGD\/SF\u2010PCL(HGSP) patches are fabricated by photochemically grafting HA and HA\u2010GRGD onto SP surfaces. The results show that the proliferations of hBMSC on HGSP patches significantly exceed those on the other patches, as determined by 3\u2010(4,5\u2010dimethylthiazol\u20102\u2010yl)\u20105\u2010(3\u2010carboxymethoxyphenyl)\u20102\u2010(4\u2010sulfophenyl)\u20102H\u2010tetrazolium assay. Notably, the formation of 5\u2010aza inducing cardiomyogenic differentiations of hBMSCMT\/HGSP patches is observed with typical sizes of \u2248317 \u03bcm wide and 26 \u03bcm high. The cardiomyogenesis of hBMSCMT\/HGSP patches including the expressions of cardiac\u2010specific genes (e.g., Gata4) and proteins (e.g., connexin43 (CX43)) significantly exceeds those of hBMSC monolayer on the HSP and SP patches. Promoting in vitro cardiomyogenesis of hBMSC with forming cardiomyogenic differentiation of hBMSCMT\/HGSP hybrid patch is possibly mediated by the synergistic functions of HA\u2010GRGD on enhancing the activity of F\u2010actin. The hBMSCMT\/HGSP cardiac patch may be further employed to cardiac tissue engineering.<jats:boxed-text content-type=\"graphic\" position=\"anchor\"><jats:graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" mimetype=\"image\/png\" position=\"anchor\" specific-use=\"enlarged-web-image\" xlink:href=\"graphic\/mabi201600173-abs-0001-m.png\"><jats:alt-text>image<\/jats:alt-text><\/jats:graphic><\/jats:boxed-text>\n<\/jats:p>","DOI":"10.1002\/mabi.201600173","type":"journal-article","created":{"date-parts":[[2016,9,28]],"date-time":"2016-09-28T09:28:22Z","timestamp":1475054902000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Promoting Cardiomyogenesis of hBMSC with a Forming Self\u2010Assembly hBMSC Microtissues\/HA\u2010GRGD\/SF\u2010PCL Cardiac Patch Is Mediated by the Synergistic Functions of HA\u2010GRGD"],"prefix":"10.1002","volume":"17","author":[{"given":"Tze\u2010Wen","family":"Chung","sequence":"first","affiliation":[{"name":"Department of Biomedical Engineering National Yang\u2010Ming University  Taipei 11221 Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hsin\u2010Yu","family":"Lo","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering National Yang\u2010Ming University  Taipei 11221 Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tzung\u2010Han","family":"Chou","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering National Yunlin University of Science and Technology  Yunlin 64402 Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jan\u2010Hou","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering National Yunlin University of Science and Technology  Yunlin 64402 Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shoei\u2010Shen","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of SurgeryNational Taiwan University Hospital National Taiwan University College of Medicine  Taipei 110 Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2016,9,28]]},"reference":[{"doi-asserted-by":"publisher","key":"e_1_2_6_1_1","DOI":"10.1002\/mabi.201500396"},{"doi-asserted-by":"publisher","key":"e_1_2_6_2_1","DOI":"10.1161\/CIRCRESAHA.108.175158"},{"doi-asserted-by":"publisher","key":"e_1_2_6_3_1","DOI":"10.1634\/stemcells.2007-0826"},{"doi-asserted-by":"publisher","key":"e_1_2_6_4_1","DOI":"10.1532\/IJH97.07041"},{"doi-asserted-by":"publisher","key":"e_1_2_6_5_1","DOI":"10.1016\/j.biomaterials.2012.12.021"},{"doi-asserted-by":"publisher","key":"e_1_2_6_6_1","DOI":"10.1016\/j.biomaterials.2013.04.034"},{"doi-asserted-by":"publisher","key":"e_1_2_6_7_1","DOI":"10.1089\/ten.teb.2013.0537"},{"doi-asserted-by":"publisher","key":"e_1_2_6_8_1","DOI":"10.1073\/pnas.1008117107"},{"doi-asserted-by":"publisher","key":"e_1_2_6_9_1","DOI":"10.1016\/j.biomaterials.2010.12.035"},{"doi-asserted-by":"publisher","key":"e_1_2_6_10_1","DOI":"10.1002\/stem.1191"},{"doi-asserted-by":"publisher","key":"e_1_2_6_11_1","DOI":"10.1016\/j.actbio.2015.11.006"},{"doi-asserted-by":"publisher","key":"e_1_2_6_12_1","DOI":"10.1016\/j.biomaterials.2006.09.009"},{"doi-asserted-by":"publisher","key":"e_1_2_6_13_1","DOI":"10.1016\/j.biomaterials.2012.04.030"},{"doi-asserted-by":"publisher","key":"e_1_2_6_14_1","DOI":"10.1002\/mame.201500028"},{"doi-asserted-by":"publisher","key":"e_1_2_6_15_1","DOI":"10.18063\/IJB.2016.01.006"},{"doi-asserted-by":"publisher","key":"e_1_2_6_16_1","DOI":"10.1016\/j.actbio.2009.12.033"},{"doi-asserted-by":"publisher","key":"e_1_2_6_17_1","DOI":"10.18063\/IJB.2016.01.005"},{"doi-asserted-by":"publisher","key":"e_1_2_6_18_1","DOI":"10.1093\/eurjhf\/hfn017"},{"doi-asserted-by":"publisher","key":"e_1_2_6_19_1","DOI":"10.1002\/jbm.a.30225"},{"doi-asserted-by":"publisher","key":"e_1_2_6_20_1","DOI":"10.1016\/j.biomaterials.2010.09.023"},{"doi-asserted-by":"publisher","key":"e_1_2_6_21_1","DOI":"10.1002\/adhm.201500154"},{"doi-asserted-by":"publisher","key":"e_1_2_6_22_1","DOI":"10.1016\/j.biomaterials.2004.02.047"},{"doi-asserted-by":"publisher","key":"e_1_2_6_23_1","DOI":"10.1002\/mabi.200700030"},{"doi-asserted-by":"publisher","key":"e_1_2_6_24_1","DOI":"10.1097\/01.blo.0000205879.50834.fe"},{"doi-asserted-by":"publisher","key":"e_1_2_6_25_1","DOI":"10.1016\/j.biomaterials.2009.03.057"},{"doi-asserted-by":"publisher","key":"e_1_2_6_26_1","DOI":"10.1016\/j.progpolymsci.2013.09.002"},{"doi-asserted-by":"publisher","key":"e_1_2_6_27_1","DOI":"10.1371\/journal.pone.0121155"},{"doi-asserted-by":"publisher","key":"e_1_2_6_28_1","DOI":"10.1074\/jbc.R100037200"},{"doi-asserted-by":"publisher","key":"e_1_2_6_29_1","DOI":"10.1111\/j.1743-6109.2006.00119.x"},{"doi-asserted-by":"publisher","key":"e_1_2_6_30_1","DOI":"10.1016\/j.carbpol.2012.04.057"},{"doi-asserted-by":"publisher","key":"e_1_2_6_31_1","DOI":"10.1016\/j.jss.2009.09.052"},{"doi-asserted-by":"publisher","key":"e_1_2_6_32_1","DOI":"10.1016\/j.biomaterials.2009.09.096"},{"doi-asserted-by":"publisher","key":"e_1_2_6_33_1","DOI":"10.1016\/j.biomaterials.2010.08.017"},{"doi-asserted-by":"publisher","key":"e_1_2_6_34_1","DOI":"10.1016\/S0142-9612(02)00231-4"},{"doi-asserted-by":"publisher","key":"e_1_2_6_35_1","DOI":"10.1016\/S0142-9612(03)00361-2"},{"doi-asserted-by":"publisher","key":"e_1_2_6_36_1","DOI":"10.1371\/journal.pone.0034960"},{"doi-asserted-by":"publisher","key":"e_1_2_6_37_1","DOI":"10.1016\/j.biomaterials.2012.08.069"},{"doi-asserted-by":"publisher","key":"e_1_2_6_38_1","DOI":"10.1111\/j.1525-1594.2006.00178.x"},{"doi-asserted-by":"publisher","key":"e_1_2_6_39_1","DOI":"10.1007\/s10856-012-4764-6"},{"doi-asserted-by":"publisher","key":"e_1_2_6_40_1","DOI":"10.1046\/j.1525-1594.2001.025008617.x"},{"doi-asserted-by":"publisher","key":"e_1_2_6_41_1","DOI":"10.1016\/j.colsurfb.2008.06.014"},{"doi-asserted-by":"publisher","key":"e_1_2_6_42_1","DOI":"10.1016\/j.memsci.2007.09.068"},{"key":"e_1_2_6_43_1","first-page":"225","volume-title":"Principle of Tissue Engineering","author":"Lanza R. P.","year":"1997"},{"doi-asserted-by":"publisher","key":"e_1_2_6_44_1","DOI":"10.1016\/j.eurpolymj.2015.08.025"},{"doi-asserted-by":"publisher","key":"e_1_2_6_45_1","DOI":"10.1039\/b927168f"},{"doi-asserted-by":"publisher","key":"e_1_2_6_46_1","DOI":"10.1016\/j.biomaterials.2007.10.048"},{"doi-asserted-by":"publisher","key":"e_1_2_6_47_1","DOI":"10.1089\/ten.2006.12.2729"},{"doi-asserted-by":"publisher","key":"e_1_2_6_48_1","DOI":"10.1074\/jbc.M411082200"},{"doi-asserted-by":"publisher","key":"e_1_2_6_49_1","DOI":"10.1089\/scd.2008.0292"},{"key":"e_1_2_6_50_1","first-page":"242","volume":"19","author":"Xing Y.","year":"2003","journal-title":"Fayixue Zazhi"},{"doi-asserted-by":"publisher","key":"e_1_2_6_51_1","DOI":"10.1016\/j.tibtech.2012.12.003"},{"doi-asserted-by":"publisher","key":"e_1_2_6_52_1","DOI":"10.1016\/j.biomaterials.2009.01.030"},{"doi-asserted-by":"publisher","key":"e_1_2_6_53_1","DOI":"10.1038\/nrm2593"},{"doi-asserted-by":"publisher","key":"e_1_2_6_54_1","DOI":"10.1634\/stemcells.2005-0186"},{"doi-asserted-by":"publisher","key":"e_1_2_6_55_1","DOI":"10.1083\/jcb.200310024"},{"doi-asserted-by":"publisher","key":"e_1_2_6_56_1","DOI":"10.1155\/2012\/346972"},{"doi-asserted-by":"publisher","key":"e_1_2_6_57_1","DOI":"10.1016\/j.biomaterials.2013.01.091"},{"doi-asserted-by":"publisher","key":"e_1_2_6_58_1","DOI":"10.1083\/jcb.153.7.1427"}],"container-title":["Macromolecular Bioscience"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fmabi.201600173","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fmabi.201600173","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/mabi.201600173","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,6]],"date-time":"2023-10-06T14:53:26Z","timestamp":1696604006000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/mabi.201600173"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,9,28]]},"references-count":58,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2017,3]]}},"alternative-id":["10.1002\/mabi.201600173"],"URL":"https:\/\/doi.org\/10.1002\/mabi.201600173","archive":["Portico"],"relation":{},"ISSN":["1616-5187","1616-5195"],"issn-type":[{"type":"print","value":"1616-5187"},{"type":"electronic","value":"1616-5195"}],"subject":[],"published":{"date-parts":[[2016,9,28]]},"article-number":"1600173"}}