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A controlled drug release at the target site can improve the efficacy and toxicity of antileishmanial drugs in clinical use. Amastigotes live and grow inside the parasitophorous vacuole of host resident macrophages. Therefore, antileishmanial drugs should accumulate in this compartment to kill the parasite and do not produce toxicity to the cell host. PEGylated dendritic polyglycerol conjugates (PG\u2010PEG) can ensure a controlled drug release and the immune activation efficiency of the host. A dendritic PG conjugate with doxorubicin (DOX) attached through a pH\u2010cleavable hydrazone linker (PG\u2010DOX(pH)\u2010PEG), is tested on murine macrophage cell lines and on ex vivo infected BALB\/c splenocytes. As a control, a dendritic PG conjugate attached via a non\u2010cleavable linker (PG\u2010DOX(non)\u2010PEG) is used. DOX fluorescence is useful to monitor the fate of the drug inside the infected cells by flow cytometry and confocal microscopy. 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