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In this study, a polymeric contrast agent for the superfine imaging of the cerebrovasculature is presented. Eight\u2010arm polyethylene glycol with a molecular weight of \u224817 000 Da conjugated with a Gd chelate and fluorescein (F\u20108\u2010arm PEG\u2010Gd) is used. The relaxivity rate is 9.3 \u00d7 10<jats:sup>\u22123<\/jats:sup><jats:sc>m<\/jats:sc><jats:sup>\u22121<\/jats:sup> s<jats:sup>\u22121<\/jats:sup>, which is threefold higher than that of free Gd chelate. Light scattering analysis reveals that F\u20108\u2010arm PEG\u2010Gd is formed by self\u2010assembly. When the F\u20108\u2010arm PEG\u2010Gd is intravenously injected, cerebrovasculature as small as 100 \u00b5m in diameter is clearly visualized. However, signals are not enhanced when Gd chelate and Gd chelate\u2010conjugated 8\u2010arm PEG are injected. Furthermore, small vasculature around infarct region in rat stroke model can be visualized. These results suggest that F\u20108\u2010arm PEG\u2010Gd enhances the MR imaging of cerebrovasculature.<\/jats:p>","DOI":"10.1002\/mabi.201700391","type":"journal-article","created":{"date-parts":[[2018,4,17]],"date-time":"2018-04-17T17:49:19Z","timestamp":1523987359000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Superfine Magnetic Resonance Imaging of the Cerebrovasculature Using Self\u2010Assembled Branched Polyethylene Glycol\u2013Gd Contrast Agent"],"prefix":"10.1002","volume":"18","author":[{"given":"Atsushi","family":"Mahara","sequence":"first","affiliation":[{"name":"Department of Biomedical Engineering National Cerebral and Cardiovascular Center Research Institute  Fujishiro\u2010dai Suita Osaka 565\u20108565 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jun\u2010ichiro","family":"Enmi","sequence":"additional","affiliation":[{"name":"Department of Investigative Radiology National Cerebral and Cardiovascular Center Research Institute  Fujishiro\u2010dai Suita Osaka 565\u20108565 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yu\u2010I","family":"Hsu","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering National Cerebral and Cardiovascular Center Research Institute  Fujishiro\u2010dai Suita Osaka 565\u20108565 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Naoki","family":"Kobayashi","sequence":"additional","affiliation":[{"name":"Faculty of Chemistry Materials and Bioengineering Kansai University  3\u20103\u201035 Yamatecho Suita Osaka 565\u20108680 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshiaki","family":"Hirano","sequence":"additional","affiliation":[{"name":"Faculty of Chemistry Materials and Bioengineering Kansai University  3\u20103\u201035 Yamatecho Suita Osaka 565\u20108680 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hidehiro","family":"Iida","sequence":"additional","affiliation":[{"name":"Department of Investigative Radiology National Cerebral and Cardiovascular Center Research Institute  Fujishiro\u2010dai Suita Osaka 565\u20108565 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0225-2407","authenticated-orcid":false,"given":"Tetsuji","family":"Yamaoka","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering National Cerebral and Cardiovascular Center Research Institute  Fujishiro\u2010dai Suita Osaka 565\u20108565 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,4,17]]},"reference":[{"key":"e_1_2_7_1_1","doi-asserted-by":"publisher","DOI":"10.16977\/cbfm.26.2_1"},{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1552-6569.2011.00583.x"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.2214\/AJR.05.0523"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0034496"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep10178"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1118\/1.2757088"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/nphoton.2014.166"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/brain\/awh191"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1212\/01.wnl.0000219767.49705.9c"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1159\/000362719"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/nbm.1396"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroimage.2011.12.055"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0121051"},{"key":"e_1_2_7_14_1","first-page":"30019","volume":"1053","author":"Park C. 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