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Four different activated polymers (mPEG\u2010aldehyde, mPEG\u2010NHS, maltodextrin\u2010aldehyde, carboxymethyl cellulose aldehyde) are conjugated to the surface of protease, \u03b1\u2010amylase, and lipase using two different strategies (reductive amination and alkylation with NHS\u2010activated acid). Although the chemical modification of the enzymes is accompanied by losses in enzyme activity (maximum loss 40%), the covalent attachment of polymers increases the thermal stability and the stability in a standard detergent formulation compared to the unmodified enzymes. The enzyme\u2013polymer conjugates are characterized by asymmetrical\u2010flow field\u2010flow fractionation and differential scanning microcalorimetry. Furthermore, it is demonstrated that conjugated enzymes still show performance in a real washing process. Enzyme\u2013polymer conjugates show a potential as a stabilizing system for enzymes in detergents.<\/jats:p>","DOI":"10.1002\/mabi.201800095","type":"journal-article","created":{"date-parts":[[2018,6,5]],"date-time":"2018-06-05T18:42:41Z","timestamp":1528224161000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Enzyme\u2013Polymer Conjugates to Enhance Enzyme Shelf Life in a Liquid Detergent Formulation"],"prefix":"10.1002","volume":"18","author":[{"given":"Sonja","family":"K\u00fcbelbeck","sequence":"first","affiliation":[{"name":"BASF SE  Carl\u2010Bosch\u2010Stra\u00dfe 38 67056 Ludwigshafen Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jules","family":"Mikhael","sequence":"additional","affiliation":[{"name":"BASF SE  Carl\u2010Bosch\u2010Stra\u00dfe 38 67056 Ludwigshafen Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Harald","family":"Keller","sequence":"additional","affiliation":[{"name":"BASF SE  Carl\u2010Bosch\u2010Stra\u00dfe 38 67056 Ludwigshafen Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rupert","family":"Konradi","sequence":"additional","affiliation":[{"name":"BASF SE  Carl\u2010Bosch\u2010Stra\u00dfe 38 67056 Ludwigshafen Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Annette","family":"Andrieu\u2010Brunsen","sequence":"additional","affiliation":[{"name":"Ernst\u2010Berl\u2010Institut f\u00fcr Technische und Makromolekulare Chemie Technische Universit\u00e4t Darmstadt  Alarich\u2010Weiss\u2010Stra\u00dfe 64287 Darmstadt Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Grit","family":"Baier","sequence":"additional","affiliation":[{"name":"BASF SE  Carl\u2010Bosch\u2010Stra\u00dfe 38 67056 Ludwigshafen Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,6,5]]},"reference":[{"key":"e_1_2_6_1_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrd2399"},{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-014-5799-z"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0169-409X(03)00108-X"},{"key":"e_1_2_6_4_1","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1007\/978-1-60761-609-2_11","volume-title":"Cancer Nanotechnology: Methods and Protocols","author":"Chan J. 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