{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T02:31:30Z","timestamp":1789957890225,"version":"4.0.1"},"reference-count":42,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2018,12,13]],"date-time":"2018-12-13T00:00:00Z","timestamp":1544659200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100011199","name":"FP7 Ideas: European Research Council","doi-asserted-by":"publisher","award":["647426"],"award-info":[{"award-number":["647426"]}],"id":[{"id":"10.13039\/100011199","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003246","name":"Nederlandse Organisatie voor Wetenschappelijk Onderzoek","doi-asserted-by":"publisher","award":["022.006.003"],"award-info":[{"award-number":["022.006.003"]}],"id":[{"id":"10.13039\/501100003246","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Macromolecular Bioscience"],"published-print":{"date-parts":[[2019,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    The increasing prevalence of end\u2010stage renal disease and persistent shortage of donor organs call for alternative therapies for kidney patients. Dialysis remains an inferior treatment as clearance of large and protein\u2010bound waste products depends on active tubular secretion. Biofabricated tissues could make a valuable contribution, but kidneys are highly intricate and multifunctional organs. Depending on the therapeutic objective, suitable cell sources and scaffolds must be selected. This study provides a proof\u2010of\u2010concept for stand\u2010alone kidney tubule grafts with suitable mechanical properties for future implantation purposes. Porous tubular nanofiber scaffolds are fabricated by electrospinning 12%, 16%, and 20% poly\u2010\u03b5\u2010caprolactone (PCL) v\/w (chloroform and dimethylformamide, 1:3) around 0.7 mm needle templates. The resulting scaffolds consist of 92%, 69%, and 54% nanofibers compared to microfibers, respectively. After biofunctionalization with L\u20103,4\u2010dihydroxyphenylalanine and collagen IV, 10\u00a0\u00d7\u00a010\n                    <jats:sup>6<\/jats:sup>\n                    proximal tubule cells per mL are injected and cultured until experimental readout. A human\u2010derived cell model can bridge all fiber\u2010to\u2010fiber distances to form a monolayer, whereas small\u2010sized murine cells form monolayers on dense nanofiber meshes only. Fabricated constructs remain viable for at least 3 weeks and maintain functionality as shown by inhibitor\u2010sensitive transport activity, which suggests clearance capacity for both negatively and positively charged solutes.\n                  <\/jats:p>","DOI":"10.1002\/mabi.201800412","type":"journal-article","created":{"date-parts":[[2018,12,13]],"date-time":"2018-12-13T04:10:51Z","timestamp":1544674251000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":35,"title":["Fabrication of Kidney Proximal Tubule Grafts Using Biofunctionalized Electrospun Polymer Scaffolds"],"prefix":"10.1002","volume":"19","author":[{"given":"Katja","family":"Jansen","sequence":"first","affiliation":[{"name":"Division of Pharmacology Utrecht Institute for Pharmaceutical Sciences Utrecht University  Universiteitsweg 99, 3584 CG Utrecht The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4269-5889","authenticated-orcid":false,"given":"Miguel","family":"Castilho","sequence":"additional","affiliation":[{"name":"Department of Orthopaedics University Medical Center Utrecht Utrecht University  P.O. Box 85500, 3508 GA Utrecht The Netherlands"},{"name":"Regenerative Medicine Center Utrecht  Uppsalalaan 8, 3584 CT Utrecht The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sanne","family":"Aarts","sequence":"additional","affiliation":[{"name":"Division of Pharmacology Utrecht Institute for Pharmaceutical Sciences Utrecht University  Universiteitsweg 99, 3584 CG Utrecht The Netherlands"},{"name":"Department of Orthopaedics University Medical Center Utrecht Utrecht University  P.O. Box 85500, 3508 GA Utrecht The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael M.","family":"Kaminski","sequence":"additional","affiliation":[{"name":"University Medical Center Freiburg Zentrale Klinische Forschung  Breisacher Stra\u00dfe 66, 79106 Freiburg im Breisgau Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2963-6865","authenticated-orcid":false,"given":"Soeren S.","family":"Lienkamp","sequence":"additional","affiliation":[{"name":"University Medical Center Freiburg Zentrale Klinische Forschung  Breisacher Stra\u00dfe 66, 79106 Freiburg im Breisgau Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roman","family":"Pichler","sequence":"additional","affiliation":[{"name":"University Medical Center Freiburg Zentrale Klinische Forschung  Breisacher Stra\u00dfe 66, 79106 Freiburg im Breisgau Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9241-7676","authenticated-orcid":false,"given":"Jos","family":"Malda","sequence":"additional","affiliation":[{"name":"Department of Orthopaedics University Medical Center Utrecht Utrecht University  P.O. Box 85500, 3508 GA Utrecht The Netherlands"},{"name":"Regenerative Medicine Center Utrecht  Uppsalalaan 8, 3584 CT Utrecht The Netherlands"},{"name":"Department of Equine Sciences Room G05228 University Medical Center Utrecht Utrecht University  Heidelberglaan 100, 3584 CX Utrecht The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6047-5900","authenticated-orcid":false,"given":"Tina","family":"Vermonden","sequence":"additional","affiliation":[{"name":"Division of Pharmacology Utrecht Institute for Pharmaceutical Sciences Utrecht University  Universiteitsweg 99, 3584 CG Utrecht The Netherlands"},{"name":"Division of Pharmaceutics Utrecht Institute for Pharmaceutical Sciences Utrecht University  Universiteitsweg 99, 3584 CG Utrecht The Netherlands"},{"name":"Regenerative Medicine Center Utrecht  Uppsalalaan 8, 3584 CT Utrecht The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jitske","family":"Jansen","sequence":"additional","affiliation":[{"name":"Department of Pathology and Pediatric Nephrology RIMLS RIHS Radboud University Medical Center  P.O. 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