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To correlate their chemical structures with cell activities, in this study, poly[(ethyl alanato)<jats:sub>0.3<\/jats:sub>(ethyl glycinato)<jats:sub>0.7<\/jats:sub>phosphazene] (PAGP) and poly[(ethyl phenylalanato)<jats:sub>0.3<\/jats:sub>(ethyl glycinato)<jats:sub>0.7<\/jats:sub>phosphazene] (PPGP) are synthesized to carry out studies on cell osteogenic differentiation. In the non\u2010contact culture manner, bone mesenchymal stromal cells (BMSCs) are cultured in transwell chambers containing PAGP or PPGP films, while the cells and the materials do not contact. In the contact culture manner, BMSCs are cultured on the PAGP or PPGP films. In the meantime, solutions containing PAGP or PPGP degradation products (i.e., phosphate, ammonium, and corresponding amino acids) are applied for cell culture using inorganic phosphate (Pi) ion as control. Thus, the influences from substrate surface and degradation products can be identified separately. The results reveal that both the phosphorus\u2010containing surface of PAGP and PPGP films and their degradation products play significant roles in regulating cell behaviors. In comparison with PAGP, PPGP seems able to provide relatively stable phosphorus\u2010containing surface to strengthen the cell\u2010scaffold interaction because of its slower degradation rate and higher Young's modulus, leading to greater promotion in osteogenic differentiation via contact effect.<\/jats:p>","DOI":"10.1002\/mabi.201800464","type":"journal-article","created":{"date-parts":[[2019,5,3]],"date-time":"2019-05-03T12:43:57Z","timestamp":1556887437000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Molecular Mechanism Study on Effect of Biodegradable Amino Acid Ester\u2013Substituted Polyphosphazenes in Stimulating Osteogenic Differentiation"],"prefix":"10.1002","volume":"19","author":[{"given":"Zhaohui","family":"Huang","sequence":"first","affiliation":[{"name":"State Key Laboratory of Organic\u2013Inorganic Composites Beijing Laboratory of Biomedical Materials Beijing University of Chemical Technology  Beijing 100029 P. 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