{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,8]],"date-time":"2025-10-08T16:22:10Z","timestamp":1759940530938,"version":"3.41.2"},"reference-count":38,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2019,6,21]],"date-time":"2019-06-21T00:00:00Z","timestamp":1561075200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100010583","name":"Universit\u00e4t Konstanz","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100010583","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Macromolecular Bioscience"],"published-print":{"date-parts":[[2020,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Atherosclerosis is a widespread and hazardous disease characterized by the formation of arterial plaques mostly composed of fat, cholesterol, and calcium ions. The direct solubilization of cholesterol represents a promising, atheroprotective strategy to subside lipid blood levels and reverse atherosclerosis. This study deals with the in\u2010depth analysis of polymer\u2010mediated cholesterol dissolution inside living human cells. To this end, a recently described multifunctional block\u2010polymer is labeled with Rhodamine B (RhoB) to investigate its interaction with cells via fluorescence microscopy. This gives insight into the cellular internalization process of the polymer, which appears to be clathrin\u2010 and caveolae\/raft\u2010dependent endocytosis. In cell single particle tracking reveals an active transport of RhoB polymer including structures. F\u00f6rster resonance energy transfer (FRET) measurements of cells treated with a fluorophore\u2010tagged cholesterol derivative and the RhoB polymer indicates the uptake of cholesterol by the polymeric particles. Hence, these results present a first step toward possible applications of cholesterol\u2010absorbing polymers for treating atherosclerosis.<\/jats:p>","DOI":"10.1002\/mabi.201900081","type":"journal-article","created":{"date-parts":[[2019,6,21]],"date-time":"2019-06-21T08:31:19Z","timestamp":1561105879000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Visualizing Cholesterol Uptake by Self\u2010Assembling Rhodamine B\u2010Labeled Polymer Inside Living Cells via FLIM\u2010FRET Microscopy"],"prefix":"10.1002","volume":"20","author":[{"given":"Franziska","family":"Doll","sequence":"first","affiliation":[{"name":"Physical Chemistry Department of Chemistry University of Konstanz  Universit\u00e4tsstra\u00dfe 10 78457 Konstanz Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Philipp","family":"Keckeis","sequence":"additional","affiliation":[{"name":"Physical Chemistry Department of Chemistry University of Konstanz  Universit\u00e4tsstra\u00dfe 10 78457 Konstanz Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patricia","family":"Scheel","sequence":"additional","affiliation":[{"name":"Physical Chemistry Department of Chemistry University of Konstanz  Universit\u00e4tsstra\u00dfe 10 78457 Konstanz Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1148-0308","authenticated-orcid":false,"given":"Helmut","family":"C\u00f6lfen","sequence":"additional","affiliation":[{"name":"Physical Chemistry Department of Chemistry University of Konstanz  Universit\u00e4tsstra\u00dfe 10 78457 Konstanz Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,6,21]]},"reference":[{"key":"e_1_2_7_1_1","doi-asserted-by":"publisher","DOI":"10.1093\/eurheartj\/ehw334"},{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1039\/c3an01050c"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmat3627"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.2174\/1876506801205010013"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molmed.2013.12.001"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/adfm.201808331"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1021\/jo052029x"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0854.2008.00801.x"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chemphyslip.2011.01.004"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201503183"},{"key":"e_1_2_7_11_1","first-page":"283","volume-title":"Methods in Enzymology","author":"Doll F.","year":"2018"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.a.20013"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1562\/0031-8655(2002)0750327FQYATR2.0.CO2"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2011.364"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00396-004-1131-2"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10895-014-1368-1"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2016.01.034"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1088\/0957-4484\/21\/35\/355102"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/mt.2009.281"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1021\/bm301281k"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1039\/C4BM00224E"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.biochem.78.081307.110540"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.4161\/cl.23967"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200908086"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.123.5.1107"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/mtna.2015.59"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/mt.2011.102"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.emboj.7601720"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bpj.2009.02.045"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1104298108"},{"key":"e_1_2_7_31_1","unstructured":"C.Rosazza PhD thesis\/dissertation Internalization and intracellular trafficking of plasmid DNA delivered by electroporation in vitro University of Konstanz (Konstanz) 2014."},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2005.10.039"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bpj.2010.08.025"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1088\/1478-3975\/9\/4\/046003"},{"volume-title":"Assay Guidance Manual","year":"2004","author":"Riss T. L.","key":"e_1_2_7_35_1"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1002\/bies.201100086"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1039\/b412924p"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2010.01.065"}],"container-title":["Macromolecular Bioscience"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fmabi.201900081","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/mabi.201900081","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/mabi.201900081","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/mabi.201900081","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,7]],"date-time":"2023-09-07T17:03:55Z","timestamp":1694106235000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/mabi.201900081"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,6,21]]},"references-count":38,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2020,1]]}},"alternative-id":["10.1002\/mabi.201900081"],"URL":"https:\/\/doi.org\/10.1002\/mabi.201900081","archive":["Portico"],"relation":{},"ISSN":["1616-5187","1616-5195"],"issn-type":[{"type":"print","value":"1616-5187"},{"type":"electronic","value":"1616-5195"}],"subject":[],"published":{"date-parts":[[2019,6,21]]},"assertion":[{"value":"2019-03-12","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-06-21","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"1900081"}}